Notes on the Life Cycle of the Tobacco Hornworm, Manduca sexta (L.) (Lepidoptera; Sphingidae), in Puerto Rico

Casanova, R. Ingles; Gaud, Silverio Medina

doi:10.46429/jaupr.v59i1.10629

Cited by 3 publications

(5 citation statements)

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“…Manduca sexta eggs were obtained from Great Lakes Hornworm (Romeo, MI, USA), and caterpillars were reared in individual containers on a wheat germ-based artificial diet (Table S2) (derived from [25][26][27][28][29][30]) at 28 • C and 40% relative humidity with a 16:8 h light:dark photoperiod. Third-instar caterpillars (head capsule width 3.1 to 3.5 mm [31]) were monitored at the head slip transition stage for molting. Newly eclosed 4th-instar caterpillars were fed for 24 h and then split into "starved" or "fed" 24 h and 48 h groups.…”

Section: Sample Collectionmentioning

confidence: 99%

Starvation-Induced Changes to the Midgut Proteome and Neuropeptides in Manduca sexta

Kaur,

Quilici,

Woolsey

et al. 2024

Insects

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Starvation is a complex physiological state that induces changes in protein expression to ensure survival. The insect midgut is sensitive to changes in dietary content as it is at the forefront of communicating information about incoming nutrients to the body via hormones. Therefore, a DIA proteomics approach was used to examine starvation physiology and, specifically, the role of midgut neuropeptide hormones in a representative lepidopteran, Manduca sexta. Proteomes were generated from midguts of M. sexta fourth-instar caterpillars, starved for 24 h and 48 h, and compared to fed controls. A total of 3047 proteins were identified, and 854 of these were significantly different in abundance. KEGG analysis revealed that metabolism pathways were less abundant in starved caterpillars, but oxidative phosphorylation proteins were more abundant. In addition, six neuropeptides or related signaling cascade proteins were detected. Particularly, neuropeptide F1 (NPF1) was significantly higher in abundance in starved larvae. A change in juvenile hormone-degrading enzymes was also detected during starvation. Overall, our results provide an exploration of the midgut response to starvation in M. sexta and validate DIA proteomics as a useful tool for quantifying insect midgut neuropeptide hormones.

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Section: Sample Collectionmentioning

confidence: 99%

Starvation-Induced Changes to the Midgut Proteome and Neuropeptides in Manduca sexta

Kaur,

Quilici,

Woolsey

et al. 2024

Insects

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“…Rare and/or charismatic species have been subject to more detailed life history studies, as this knowledge is imperative for their conservation (e.g., Homziak & Homziak, 2010; Matthews & Pérez, 2014; Torres‐Bauzá, 1991). Agricultural pests have also been of interest due to their economic significance (e.g., Inglés Casanova & Medina‐Gaud, 1975; Segarra‐Carmona et al., 2010). In addition to life history studies, brief records documenting new findings about host plants and natural enemies for lepidopteran agricultural pests are also published periodically (e.g., Cabrera‐Asencio et al., 2013; Gregory et al., 1991; LaSalle & Peña, 1997; Santiago‐Blay, 1983).…”

Section: Taxonomy and Life Historymentioning

confidence: 99%

“…Parasitism, particularly by insects of the orders Diptera and Hymenoptera, has also been a topic of interest, given its potential use in bioremediation. Wasps in the family Braconidae are especially abundant parasitoids in Puerto Rico and are frequently investigated as biological control species for agricultural applications (e.g., Borkhataria et al., 2012; Gallardo‐Covas, 1988, 2005; Inglés Casanova & Medina‐Gaud, 1975).…”

Section: Ecologymentioning

confidence: 99%

“…Life cycle stages, estimated leaf consumption, and natural enemies were investigated in Puerto Rico (Inglés Casanova & Medina‐Gaud, 1975)…”

Section: Agriculturementioning

confidence: 99%

“…Rico and are frequently investigated as biological control species for agricultural applications (e.g., Borkhataria et al, 2012;Gallardo-Covas, 1988, 2005Inglés Casanova & Medina-Gaud, 1975).…”

Section: Community-level Interactionsmentioning

confidence: 99%

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Lepidoptera research in Puerto Rico: Reconnecting with historical legacies to guide future priorities

Terry,

Alonso‐Rodríguez,

Miller

et al. 2023

Biotropica

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The Caribbean archipelago of Puerto Rico supports a diverse flora and fauna and is located in a region characterized by complex socio‐economic and environmental change. The diversity of entomofauna across Puerto Rico has received considerable attention in wide‐scale research over the last century, with particular emphasis on the order Lepidoptera as the subject of substantial taxonomic and ecological surveys. However, much of this work is incomplete, outdated, or has been obscured in gray literature. Thus, our primary objectives were to contextualize the role of past research in the current understanding of Puerto Rican Lepidoptera and to outline an agenda for future research. Specifically, we provide an overview of taxonomic, ecological, agricultural, and conservation Lepidoptera research in Puerto Rico and highlight key studies and historical datasets. We found that, despite a strong taxonomic legacy, native moth taxonomy remains poorly understood, except for a few major pests. Further, much of the recent Lepidoptera research has focused on short‐term evaluations of agricultural pests, necessitated by immediate economic needs. The current ecological status of Lepidoptera on the islands is unknown. Therefore, prioritizing ecological research could provide timely insight for understanding changing Lepidoptera diversity and distribution and for conserving this biologically and economically significant group. Greater emphasis on long‐term monitoring and digitization of museum collections would be particularly useful for quantifying past and forecasting future impacts of global change.Abstract in Spanish is available with online material.

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Manduca sexta (tobacco hornworm (USA))

2022

CABI Compendium

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Notes on the Life Cycle of the Tobacco Hornworm, Manduca sexta (L.) (Lepidoptera; Sphingidae), in Puerto Rico

Cited by 3 publications

References 1 publication

Starvation-Induced Changes to the Midgut Proteome and Neuropeptides in Manduca sexta

Starvation-Induced Changes to the Midgut Proteome and Neuropeptides in Manduca sexta

Lepidoptera research in Puerto Rico: Reconnecting with historical legacies to guide future priorities

Manduca sexta (tobacco hornworm (USA))

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