It is shown that any finitely generated subring of a global field has a universal first-order definition in its fraction field. This covers Koenigsmann's result for the ring of integers and its subsequent extensions to rings of integers in number fields and rings of S-integers in global function fields of odd characteristic. In this article a proof is presented which is uniform in all global fields, including the characteristic two case, where the result is entirely novel. Furthermore, the proposed method results in universal formulae requiring significantly fewer quantifiers than the formulae that can be derived through the previous approaches.