Notes on Metabolic Plasmid Organization and Expression

Gunsalus, I. C.

doi:10.1007/978-1-4613-2447-8_48

Cited by 4 publications

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“…A number of independent plasmids which determine the degradation of toluene, xylene, salicylate, naphthalene, and their various derivatives have been isolated (6-8, 13, 14, 36-38). The best studied pseudomonads of this group harbor a single metabolic plasmid (9,19,33,34). Multiplasmid bacterial strains that carry several metabolic plasmids can be constructed, but they are unstable (2,17,31).…”

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Degradation of phenol and m-toluate in Pseudomonas sp. strain EST1001 and its Pseudomonas putida transconjugants is determined by a multiplasmid system

Kivisaar¹,

Habicht²,

Heinaru³

1989

J Bacteriol

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The utilization of phenol, m-toluate, and salicylate (Phe+, mTol+, and Sal' characters, respectively) in Pseudomonas sp. strain EST1001 is determined by the coordinated expression of genes placed in different plasmids, i.e., by a multiplasmid system. The natural multiplasmid strain EST1001 is phenotypically unstable.In its Phe-, mTol-, and Sal-segregants, the plasmid DNA underwent structural rearrangements without a marked loss of plasmid DNA, and the majority of segregants gave revertants. The genes specifying the degradation of phenol and m-toluate were transferable to P. putida PaW340, and in this strain a new multiplasmid system with definite structural changes was formed. The 17-kilobase transposable element, a part of the TOL plasmid pWWO present in the chromosome of PaW340, was inserted into the plasmid DNA in transconjugants. In addition, transconjugant EST1020 shared pWWO-like structures. Enzyme assays demonstrated that ortho-fission reactions were used by bacteria that grew on phenol, whereas m-toluate was catabolized by a meta-fission reaction. Salicylate was a functional inducer of the enzymes of both pathways. The expression of silent metabolic pathways of phenol or m-toluate degradation has been observed in EST1001 PhemTol+ and Phe+ mTol-transconjugants. The switchover of phenol degradation from the ortho-to the meta-pathway in EST1033 also showed the flexibility of genetic material in EST1001 transconjugants.

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Degradation of phenol and m-toluate in Pseudomonas sp. strain EST1001 and its Pseudomonas putida transconjugants is determined by a multiplasmid system

Kivisaar¹,

Habicht²,

Heinaru³

1989

J Bacteriol

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“…The alginate lyase from Klebsiella pneumoniae strain PGI has an absolute specificity for L-guluronate at the non-reducing side of the bond that is cleaved (Boyd & Turvey, 1977), and this enzyme catalyzes the first reaction of the pathway for alginate catabolism. Extrachromosomal plasmid DNA is known to encode proteins and enzymes associated with many ancilliary reactions in microbial cells, and the involvement of catabolic plasmids in the utilization of a wide range of hydrocarbons is well documented (see Gunsalus, 1985). Plasmids, because of their ability to replicate independently of the chromosome and to transfer from one species to another, are of considerable importance in the generation of genotypic and phenotypic variation in microorganisms (Hardy, 1986).…”

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confidence: 99%