Note on the Variations in Area and in Staining Intensity of Red Blood Cells and on Their Correlation

Abstract

By means of a laborious technique, based on the use of a projection microscope and a sensitive electric photometer, it has been shown that both the areas and transparencies of stained red blood cells may be determined. From the few observations completed, it appears that, statistically, these attributes are positively correlated in normal human blood and their regression line is straight. In pernicious anemia the A/T correlation is negative. Cases of secondary anemia may show either positive or negative A/T correlations. The pathological blood specimens studied all showed non-linear A/T regression lines.