Notch4 overexpression in ameloblastoma correlates with the solid/multicystic phenotype

Siar, Chong Huat; Nagatsuka, Hitoshi; Chuah, Kee Seng; Rivera, Rosario Santos; Nakano, Keisuke; Ng, Kok Han; Kawakami, Toshiyuki

doi:10.1016/j.tripleo.2010.03.009

Cited by 12 publications

(16 citation statements)

References 31 publications

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“…This study also revealed that the expression patterns of Notch receptors and their ligands in the CEOT are distinctive from other odontogenic epithelial neoplasm such as the ameloblastoma 25,26) .…”

Section: Discussionmentioning

confidence: 59%

“…Human malignancies demonstrating Notch participation include cancers of the breast 12) , pancreas 13) , ovanes 14) tongue 15) , bone 28) and cartilage 29) . Recently, aberrant Notch signaling has also been implicated in the tumorigenesis of some jaw neoplasms in particular odontogenic neoplasms such as ameloblastoma 22,23,25,26) , ameloblastic fibroma 22) , squamous odontogenic tumor 24) and cemento-ossifying fibroma 35) . However its role in CEOT remains unknown.…”

Section: Discussionmentioning

confidence: 99%

“…Odontogenic neoplasms constitute an important area of research interest because many of these tumors notably ameloblastoma 22,25,26,30,31) , and odontogenic keratocysts [32][33][34] , x100); B, details of intense Jagged1 expression in amyloid-like deposits (antiJagged1, x400); C, Jagged2 is absent (anti-Jagged2, x200); D, Delta1 expression is mild to nonreactive in tumor epithelium and amyloid-like substances (anti-Delta1, x400); and E, Delta1-negative tumor showing nonreactivity throughout the epithelium and amyloid-like globules (anti-Delta1, x400).…”

Section: Discussionmentioning

confidence: 99%

“…Our group has thus far investigated the immunoexpression patterns of Notch receptors and their ligands in ameloblastomas 22,25,26) , ameloblastic fibroma 22) , ameloblastic carcinoma 22) , squamous odontogenic tumor 24) and odontogenic myxoma 27) . A search of the published literature disclosed that Notch activity in CEOT has not been elucidated.…”

Section: Mammalianmentioning

confidence: 99%

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Immunohistochemical Study of Notch Signaling Proteins in the Calcifying Epithelial Odontogenic Tumor (Pindborg Tumor)

Siar

Chuah

Nakano

et al. 2010

J. Hard Tissue Biology.

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Notch signaling pathway mediates diverse biological processes including cell fate decisions during odontogenesis. Dysregulation of Notch has been implicated in the tumorigenesis of some odontogenic neoplasms but its role in the calcifying epithelial odontogenic tumor (CEOT) remains unclarified. The aim here was to investigate Notch expression in CEOT and to speculate on its significance. Receptors Notch1-4 and their ligands (Jagged1, Jagged2 and Delta1) were examined immunohistochemically in six CEOT cases. Expression levels were quantified according to the percentage of positive tumor cells, amyloid-like proteins and calcifications: (-), negative staining; (+), mild and focal positivity <25%; (++), moderate positivity in significant areas 25-50%; (+++), strong positivity in predominant areas >50%. CEOT epithelium demonstrated variable expression levels for Notch1, 3, 4, Jagged1 and Delta1 suggesting upregulation of these molecules at sites of tumor differentiation. Distribution patterns were distinct with some overlap. Their localizations were largely membranous and/or cytoplasmic. Notch2 and Jagged2 were absent. Amyloid-like materials strongly expressed Jagged1 but variably Notch1, 3 and Delta1 implicating that these signaling proteins maybe competitive substrates with CEOT amyloid-like proteins for proteolysis. Notch2, 4 and Jagged2 were absent. Mineralized substances including Liesegang rings were negative for Notch receptors and ligands suggesting that calcification process is associated with downregulation of these molecules. Stromal endothelium and fibroblasts were stained variably positive. Taken together, current data suggest that Notch receptors and their ligands may play differing roles in the acquisition of cell fates in CEOT. Notch accumulations within amyloid-like protein suggest impaired proteolysis.

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Section: Discussionmentioning

confidence: 59%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Mammalianmentioning

confidence: 99%

See 2 more Smart Citations

Immunohistochemical Study of Notch Signaling Proteins in the Calcifying Epithelial Odontogenic Tumor (Pindborg Tumor)

Siar

Chuah

Nakano

et al. 2010

J. Hard Tissue Biology.

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“…Previously, some papers have published examination results of immunohistochemical expression of Notch and the related singals in some odontogenic neoplasms, including ameloblastoma 2,17) , desmoplastic ameloblastoma 3,26) , ameloblastic carcinoma 5,18) , and squamous odontogenic tumor 4) . These results suggest that Notch Figure 6.…”

Section: Discussionmentioning

confidence: 99%

Immunohistochemical Observation of Notch Signaling in a Case of Calcifying Cystic Odontogenic Tumor

Nakano

Siar

Tomida

et al. 2010

J. Hard Tissue Biology.

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Odontogenesis is a complex biological process both with epithelial and mesenchymal tissues, and this process directly reflects the development of odontogenic neoplasms. Therefore, we have examined Notch signaling in a case of calcifying cystic odontogenic tumor, with ameloblastic fibroma and odontogenic myxoma cases as controls. In these specimens, Notch-positive-products were present both in the epithelial and ectomesenchymal components in the calcifying cystic odontogenic tumor and the ameloblastic fibroma case, but negative in the odontogenic myxoma case, having no odontogenic epithelial islands to IHC and ISH examinations. Therefore, the examination results suggest that Notch signaling plays some important roles in cytological differentiation or acquisition of tissue-specific characteristics between odontogenic epithelium and odontogenic ectomesenchymal tissues.

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Expression of midkine in ameloblastomas and its correlation with clinicopathologic parameters

Scheper

Duarte

Intapa

et al. 2012

Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology

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Notch4 overexpression in ameloblastoma correlates with the solid/multicystic phenotype

Abstract: Present findings suggest that Notch signaling molecules may play differing roles in the acquisition of different ameloblastoma phenotypes.

Cited by 12 publications

References 31 publications

Immunohistochemical Study of Notch Signaling Proteins in the Calcifying Epithelial Odontogenic Tumor (Pindborg Tumor)

Immunohistochemical Study of Notch Signaling Proteins in the Calcifying Epithelial Odontogenic Tumor (Pindborg Tumor)

Immunohistochemical Observation of Notch Signaling in a Case of Calcifying Cystic Odontogenic Tumor

Expression of midkine in ameloblastomas and its correlation with clinicopathologic parameters

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