Notch signaling relieves the joint-suppressive activity of Defective proventriculus in the Drosophila leg

Shirai, Tetsuya; Yorimitsu, Takeshi; Kiritooshi, Naruto; Matsuzaki, Fumio; Nakagoshi, Hideki

doi:10.1016/j.ydbio.2007.09.003

Cited by 24 publications

(25 citation statements)

References 60 publications

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“…Such regulation of the membrane availability of Notch could equally affect Dlmediated activation, although Ser appears to be the major ligand responsible in the joints (Bishop et al, 1999). Other factors are likely to influence proximal repression of N because ectopic joints are also observed in alterations of the EGFR pathway (Galindo et al, 2005) and mutants of defective proventriculus (Shirai et al, 2007). We note also that the domains of N activation (both normal and ectopic) extend beyond the cells at the interface with Ser.…”

Section: Discussionmentioning

confidence: 72%

Planar cell polarity controls directional Notch signaling in theDrosophilaleg

et al. 2012

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SUMMARYThe generation of functional structures during development requires tight spatial regulation of signaling pathways. Thus, in Drosophila legs, in which Notch pathway activity is required to specify joints, only cells distal to ligand-producing cells are capable of responding. Here, we show that the asymmetric distribution of planar cell polarity (PCP) proteins correlates with this spatial restriction of Notch activation. Frizzled and Dishevelled are enriched at distal sides of each cell and hence localize at the interface with ligand-expressing cells in the non-responding cells. Elimination of PCP gene function in cells proximal to ligand-expressing cells is sufficient to alleviate the repression, resulting in ectopic Notch activity and ectopic joint formation. Mutations that compromise a direct interaction between Dishevelled and Notch reduce the efficacy of repression. Likewise, increased Rab5 levels or dominantnegative Deltex can suppress the ectopic joints. Together, these results suggest that PCP coordinates the spatial activity of the Notch pathway by regulating endocytic trafficking of the receptor.

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Section: Discussionmentioning

confidence: 72%

Planar cell polarity controls directional Notch signaling in theDrosophilaleg

et al. 2012

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“…Among GAL4 drivers inserted near the genes reported to show segmental expression in the tarsus, we found three drivers which were expressed in different but overlapping populations of cells in the joint region: big brain (bib)-GAL4, neuralized (neur)-GAL4 and fringe (fng)-GAL4 (de Celis et al, 1998;Mirth and Akam, 2002;Rauskolb and Irvine, 1999;Shirai et al, 2007). bib and neur were expressed in the deep region of the cavity, flanked by fng expression in cells projecting into the ridge (see Fig.…”

Section: Research Articlementioning

confidence: 99%

Dynamic shape changes of ECM-producing cells drive morphogenesis of ball-and-socket joints in the fly leg

et al. 2010

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SUMMARYAnimal body shape is framed by the skeleton, which is composed of extracellular matrix (ECM). Although how the body plan manifests in skeletal morphology has been studied intensively, cellular mechanisms that directly control skeletal ECM morphology remain elusive. In particular, how dynamic behaviors of ECM-secreting cells, such as shape changes and movements, contribute to ECM morphogenesis is unclear. Strict control of ECM morphology is crucial in the joints, where opposing sides of the skeleton must have precisely reciprocal shapes to fit each other. Here we found that, in the development of ball-and-socket joints in the Drosophila leg, the two sides of ECM form sequentially. We show that distinct cell populations produce the 'ball' and the 'socket', and that these cells undergo extensive shape changes while depositing ECM. We propose that shape changes of ECM-producing cells enable the sequential ECM formation to allow the morphological coupling of adjacent components. Our results highlight the importance of dynamic cell behaviors in precise shaping of skeletal ECM architecture.

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“…Inhibition of dve-A repression by the heat-shock mediated ubiquitous dve-A induction severely impairs the copper absorptive function (Nakagoshi et al, 1998). Furthermore, we have also shown that the temporally regulated dve repression is crucial for wing patterning and leg joint formation Shirai et al, 2007).…”

Section: Spatio-temporal Regulation Of Dve Isoforms Is Important For mentioning

confidence: 55%

Spatial and temporal requirement of Defective proventriculus activity during Drosophila midgut development

Nakagawa

Fujiwara-Fukuta

Yorimitsu

et al. 2011

Mechanisms of Development

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The Drosophila middle midgut cells derived from the endoderm develop into four distinct types of cell. Of these cells, copper cells have invaginated microvillar membranes on their apical surface, and they are involved in two distinct functions, i.e., copper absorption and acid secretion. The homeobox gene defective proventriculus (dve) is expressed in the midgut, and two transcripts, type A (∼4.9kb) and type B (∼3.5kb), have been identified. We isolated the deletion allele dve(E181) that completely removes the first exon for type-A (dve-A) transcript. Dve expression pattern in dve-A mutant background indicates that isoform switching is dynamically regulated in a cell-type specific manner. Using RNAi for dve-A, we examined spatial and temporal requirement of the Dve-A activity. Early Dve-A activity is required to repress isoform switching in copper cells, and for establishment of two gut functions. Late Dve-A activity in copper cells, but not in adjacent interstitial cells, is required for acid secretion, while the activity is redundantly required in both cells for the copper absorptive function. Furthermore, ectopic type-B expression in larval copper cells specifically impaired the copper absorptive function. These results provide insight into molecular mechanisms to establish functional specificity.

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Notch signaling relieves the joint-suppressive activity of Defective proventriculus in the Drosophila leg

Cited by 24 publications

References 60 publications

Planar cell polarity controls directional Notch signaling in theDrosophilaleg

Planar cell polarity controls directional Notch signaling in theDrosophilaleg

Dynamic shape changes of ECM-producing cells drive morphogenesis of ball-and-socket joints in the fly leg

Spatial and temporal requirement of Defective proventriculus activity during Drosophila midgut development

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