Notch receptor encodes two structurally separable functions in Drosophila: A genetic analysis

Wnt/Notch signalling and information processing during development

“…This sequence of events can account for some of the genetic interactions that have been observed between Notch and Wingless in Drosophila, in particular, the extreme sensitivity of the wing margin to the dosage of Notch and wingless. Since loss of one copy of Notch produces a wing phenotype, it is not surprising that, given their close association during wing development, alterations in wingless gene dosage also have dramatic effects on wing development when they occur in a background of compromised Notch signalling Langdon et al, 2006). In agreement with this, the haploinsufficient phenotype of Notch is a particularly sensitive assay for identifying proteins that interact genetically with Notch (Go and ArtavanisTsakonas, 1998;Hall et al, 2004;Mahoney et al, 2006;Verheyen et al, 1996).…”

“…However, this is not the only way in which Wnt and Notch signalling interact. Genetic analysis of pattern formation in Drosophila has uncovered instances of interactions that cannot be accounted for by the modular transcription network described above (Brennan et al, 1997;Heitzler and Simpson, 1991;Langdon et al, 2006). The most significant of these is associated with the Abruptex (Ax) and Microchaete defective (Mcd) classes of Notch alleles (Brennan et al, 1999c;Heitzler and Simpson, 1993;Martinez Arias, 2002).…”

Notch destabilises maternal β-catenin and restricts dorsal-anterior development inXenopus

“…Other ligands, distinct from the Delta-Serrate family, might participate, and even non-nuclear mechanisms, perhaps involving cytoskeleton interactions, were proposed for Notch-dependent axon guidance in Drosophila (Brennan et al, 1999;Hu et al, 2003;Hori et al, 2004;Langdon et al, 2006;Mizutani et al, 2007;D'Souza et al, 2008;Le Gall et al, 2008;D'Souza et al, 2010).…”

“…The synergistic interactions generally involve the canonical, CSL-dependent transcriptional activation of Notch-target genes (for a review, see Hayward et al, 2008). Remarkably, the CSL-independent pathway is preferentially associated with antagonism of Wingless in Drosophila (Lawrence et al, 2001;Martínez Arias et al, 2002;Langdon et al, 2006;Hayward et al, 2008). In recent years, evidence has been found that in the wing imaginal disc, Notch initially acts through a non-transcriptional pathway by promoting the degradation of Armadillo (Arm, the fly homologue of -catenin) without the intervention of GSK3 (Hayward et al, 2005;Hayward et al, 2008;Sanders et al, 2009).…”

Differentiation of the Drosophila serotonergic lineage depends on the regulation of Zfh-1 by Notch and Eagle

“…The N ICD then translocates to the nucleus and associates with the transcription factor Suppressor of Hairless (Su(H)) and other proteins to form a complex that activates gene transcription (recent reviews Bray, 2006;Le Borgne et al, 2005;Louvi and Artavanis-Tsakonas, 2006;Roegiers and Jan, 2004;Schweisguth, 2004). More recently, Notch signaling that is independent of Su(H) has been described (Fuwa et al, 2006;Hayward et al, 2005;Hori et al, 2004;Langdon et al, 2006;reviewed in Martinez Arias et al, 2002;Wilkin and Baron, 2005). The molecular mechanism of Su(H)-independent Notch signaling is uncertain and it is possible there may be more than one mechanism.…”