Notch-mediated lateral induction is necessary to maintain vestibular prosensory identity during inner ear development

Brown, Rogers M.; Nelson, Joel C.; Zhang, Hongyuan; Kiernan, Amy E.; Groves, Andrew K.

doi:10.1016/j.ydbio.2020.02.015

Cited by 12 publications

(6 citation statements)

References 67 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Therefore, downregulation of JAG-1 was induced in the cells at day 6 and 12 of otic progenitor differentiation (designated as J1-6d and J1-12d infection schemes, respectively). The expression of otic progenitor-specific genes and proteins in the J1-6d infection scheme was significantly lower than that of the control cells, which supported the previous statement "JAG-1 could stabilize prosensory fate" [34]. The GFP-positive cells in the J1-6d and J1-12d infection schemes were sorted by FACS and eventually induced to differentiate into hair cell-like cells.…”

Section: Discussionsupporting

confidence: 85%

“…Moreover, co-activation of cell cycle activator and Notch effectively transdifferentiated mature supporting cells into hair cells [27,28]. The prominent function of JAG-1 in Notch signaling is lateral induction, which is essential for the proliferation and development of OPCs and is necessary for hair cell differentiation [29][30][31][32][33][34]. Macchiarulo et al found that knocking out JAG-1 in mouse ear canal vesicles led to failure of semicircular tube development and reduction of hair cells [35].…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Promotion of In Vitro Hair Cell-like Cell Differentiation from Human Embryonic Stem Cells through the Regulation of Notch Signaling

et al. 2021

View full text Add to dashboard Cite

The Notch signaling pathway plays an important role in otic neurogenesis by regulating the differentiation of inner ear hair cells and supporting cells. Notch-regulated differentiation is required for the regeneration of hair cells in the inner ear. The temporal expression pattern of Notch ligands and receptors during in vitro hair cell-like cell differentiation from human embryonic stem cells (hESCs) was detected by quantitative reverse transcription-polymerase chain reaction (qRT-PCR). Subsequently, pAJ-U6-shRNA-CMV-Puro/GFP recombinant lentiviral vectors encoding short hairpin RNAs were used to silence JAG-1, JAG-2, and DLL-1, according to the temporal expression pattern of Notch ligands. Then, the effect of each ligand on the in vitro differentiation of hair cells was examined by RT-PCR, immunofluorescence, and scanning electron microscopy (SEM). The results showed that the individual deletion of JAG-2 or DLL-1 had no significant effect on the differentiation of hair cell-like cells. However, the simultaneous inhibition of both DLL-1 and JAG-2 increased the number of hair cell-like cells and decreased the number of supporting cells. JAG-2 and DLL-1 may have a synergistic role in in vitro hair cell differentiation.

show abstract

Section: Discussionsupporting

confidence: 85%

Section: Discussionmentioning

confidence: 99%

Promotion of In Vitro Hair Cell-like Cell Differentiation from Human Embryonic Stem Cells through the Regulation of Notch Signaling

et al. 2021

View full text Add to dashboard Cite

show abstract

“…JAG1's role in repressing a Hensen's cellto-Claudius cell-fate switch is reminiscent of its early role in vestibular prosensory cell maintenance. There, JAG1-Notch signaling prevents the conversion of prosensory progenitors into nonsensory cells through maintaining the expression of the transcription factor SOX2 and through antagonizing the transcription factor LMX1A (Mann et al, 2017;Brown et al, 2020). A similar antagonistic relationship between Claudius cell-specific genes and JAG1-SOX2 signaling may be at play, and future investigations into the molecular mechanisms of Claudius cell-fate determination are warranted.…”

Section: Discussionmentioning

confidence: 99%

The Notch Ligand Jagged1 Is Required for the Formation, Maintenance, and Survival of Hensen's Cells in the Mouse Cochlea

Chrysostomou¹,

Zhou²,

Darcy³

et al. 2020

J. Neurosci.

View full text Add to dashboard Cite

During cochlear development, the Notch ligand JAGGED 1 (JAG1) plays an important role in the specification of the prosensory region, which gives rise to sound-sensing hair cells and neighboring supporting cells (SCs). While JAG1's expression is maintained in SCs through adulthood, the function of JAG1 in SC development is unknown. Here, we demonstrate that JAG1 is essential for the formation and maintenance of Hensen's cells, a highly specialized SC subtype located at the edge of the auditory epithelium. Using Sox2 CreERT2/1 ::Jag1 loxP/loxP mice of both genders, we show that Jag1 deletion at the onset of differentiation, at embryonic day 14.5, disrupted Hensen's cell formation. Similar loss of Hensen's cells was observed when Jag1 was deleted after Hensen's cell formation at postnatal day (P) 0/P1 and fate-mapping analysis revealed that in the absence of Jag1, some Hensen's cells die, but others convert into neighboring Claudius cells. In support of a role for JAG1 in cell survival, genes involved in mitochondrial function and protein synthesis were downregulated in the sensory epithelium of P0 cochlea lacking Jag1. Finally, using Fgfr3-iCreER T2 ::Jag1 loxP/loxP mice to delete Jag1 at P0, we observed a similar loss of Hensen's cells and found that adult Jag1 mutant mice have hearing deficits at the low-frequency range.

show abstract

“…3B), perhaps through Wnt signaling activity (Jayasena et al, 2008). All these Sox2-expressing cells are competent to the prosensory fate (Hartman et al, 2010;Pan et al, 2013), but only those that maintain Notch signaling activity are specified into sensory cells (Daudet et al, 2007;Mann et al, 2017;Brown et al, 2020). Therefore, the default state of these cells is the non-sensory fate and they require Notch-dependent lateral induction in order to become prosensory cells.…”

Section: Cell Recruitment Of Prosensory Cells In the Development Of Tmentioning

confidence: 99%

Inducing your neighbors to become like you: cell recruitment in developmental patterning and growth

Muñoz-Nava¹,

Flores-Flores²,

Nahmad³

2021

Int. J. Dev. Biol.

View full text Add to dashboard Cite

Cell differentiation, proliferation, and morphogenesis are generally driven by instructive signals that are sent and interpreted by adjacent tissues, a process known as induction. Cell recruitment is a particular case of induction in which differentiated cells produce a signal that drives adjacent cells to differentiate into the same type as the inducers. Once recruited, these new cells may become inducers to continue the recruitment process, closing a feed-forward loop that propagates the growth of a specific cell-type population. So far, little attention has been given to cell recruitment as a developmental mechanism. Here, we review the components of cell recruitment and discuss its contribution to development in three different examples: the Drosophila wing, the vertebrate inner ear, and the mammalian thyroid gland. Finally, we posit some open questions about the role of cell recruitment in organ patterning and growth.

show abstract

Notch-mediated lateral induction is necessary to maintain vestibular prosensory identity during inner ear development

Cited by 12 publications

References 67 publications

Promotion of In Vitro Hair Cell-like Cell Differentiation from Human Embryonic Stem Cells through the Regulation of Notch Signaling

Promotion of In Vitro Hair Cell-like Cell Differentiation from Human Embryonic Stem Cells through the Regulation of Notch Signaling

The Notch Ligand Jagged1 Is Required for the Formation, Maintenance, and Survival of Hensen's Cells in the Mouse Cochlea

Inducing your neighbors to become like you: cell recruitment in developmental patterning and growth

Contact Info

Product

Resources

About