Notch is required for wingless signaling in the epidermis of Drosophila

Couso, Juan Pablo; Arias, Alfonso Martínez

doi:10.1016/0092-8674(94)90195-3

Cited by 169 publications

(84 citation statements)

References 53 publications

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“…Finally, Wnt-3a encodes a secreted protein and would be expected to act non-cell autonomously. Genetic studies of the Drosophila rela- vestigial tail is a hypomorphic allele of Wnt-3a (van den Heuvel et al 1993;Couso and Arias 1994) in a putative regulatory region. If the vt lesion is a point mutation in an enhancer region, it may be difficult to locate.…”

Section: Discussionmentioning

confidence: 99%

Analysis of the vestigial tail mutation demonstrates that Wnt-3a gene dosage regulates mouse axial development.

Greco¹,

Takada²,

Newhouse³

et al. 1996

Genes Dev.

234

161

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Mice homozygous for the recessive mutation vestigial tail (v-t), which arose spontaneously on Chromosome 11, exhibit vertebral abnormalities, including loss of caudal vertebrae leading to shortening of the tail. Wnt-3a, a member of the wingless family of secreted glycoproteins, maps to the same chromosome. Embryos homozygous for a null mutation in Wnt-3a (Wnt-3a "e°) have a complete absence of tail bud development and are truncated rostral to the hindlimbs. Several lines of evidence reveal that vt is a hypomorphic allele of Wnt-3a. We show that Wnt-3a and vt eosegregate in a high-resolution backcross and fail to complement, suggesting that Wnt-3a "~° and vt are allelic. Embryos heterozygous for both alleles have a phenotype intermediate between that of Wnt-3a n~° and vt homozygotes, lacking a tail, but developing thoracic and a variable number of lumbar vertebrae. Although no gross alteration in the Wnt-3a gene was detected in vt mice and the Wnt-3a coding region was normal, Wnt-3a expression was markedly reduced in vt/rt embryos consistent with a regulatory mutation in Wnt-3a. Furthermore, the analysis of allelic combinations indicates that Wnt-3a is required throughout the period of tail bud development for caudal somitogenesis. Interestingly, increasing levels of Wnt-3a activity appear to be necessary for the formation of more posterior derivatives of the paraxial mesoderm.

show abstract

Section: Discussionmentioning

confidence: 99%

Analysis of the vestigial tail mutation demonstrates that Wnt-3a gene dosage regulates mouse axial development.

Greco¹,

Takada²,

Newhouse³

et al. 1996

Genes Dev.

234

161

View full text Add to dashboard Cite

show abstract

“…This process requires cell-cell communication among groups of cells, all of which can both send and receive signals iHeitzler and Simpson 1991). Strong genetic interactions between N and wg indicate that the two genes function in the same pathway during wing margin formation (Couso and Martinez Arias 1994;Hing et al 1994). Couso and Martinez-Arias (1994) have proposed models in which wg acts upstream or parallel to N during wing margin development and may even be an N ligand; however, loss of N function on either side of the D/V boundary causes toss of wg expression, wing margin, and wing blade tissue in both compartments (de Cells and Garcia-Bellido 1994;Rulifson and Blair 1995}, suggesting a function for N upstream of wg.…”

Section: Doherty Eta|mentioning

confidence: 99%

“…Strong genetic interactions between N and wg indicate that the two genes function in the same pathway during wing margin formation (Couso and Martinez Arias 1994;Hing et al 1994). Couso and Martinez-Arias (1994) have proposed models in which wg acts upstream or parallel to N during wing margin development and may even be an N ligand; however, loss of N function on either side of the D/V boundary causes toss of wg expression, wing margin, and wing blade tissue in both compartments (de Cells and Garcia-Bellido 1994;Rulifson and Blair 1995}, suggesting a function for N upstream of wg. Kim et al (1995) and Diaz-Benjumea and Cohen (1995) have proposed that Serrate (Ser), an N ligand with sequence similarity to D1 (Fleming et al 1990;Rebay et al 1991 ), functions as a dorsal to ventral signal downstream of fng.…”

Section: Doherty Eta|mentioning

confidence: 99%

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Delta is a ventral to dorsal signal complementary to Serrate, another Notch ligand, in Drosophila wing formation.

Feger²,

et al. 1996

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Wing margin formation in Drosophila requires the Notch receptor and, in the dorsal compartment, one of its ligands, Serrate. We provide evidence that Delta, the other known ligand for Notch, is also essential for this process. Delta is required in ventral cells at the dorsal/ventral compartment boundary, where its expression is specifically elevated in second-instar wing discs during wing margin formation. Moreover, ectopic Delta expression induces wingless, vestigial, and cut and causes adult wing tissue outgrowth in the dorsal compartment. This effect is mediated by Notch, because loss of Notch activity suppresses Delta-induced ectopic wing outgrowth. Whereas ectopic expression of Notch or the truncated activated Notch induces cut in both dorsal and ventral compartments, ectopic Delta expression induces cut only in the dorsal compartment and ectopic Serrate induces cut only in the ventral compartment. These observations indicate that Notch-expressing cells in a given compartment have different responses to Delta and Serrate. We propose that Delta and Serrate function as compartment-specific signals in the wing disc, to activate Notch and induce downstream genes required for wing formation.

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“…Slp and Notch effectors may converge on the twist promoter to regulate expression. Additionally, Wingless signaling components may directly regulate and/or inhibit Notch (Axelrod et al, 1996;Couso and Martinez Arias, 1994;Foltz et al, 2002;Ramain et al, 2001;Strutt et al, 2002).…”

Section: Notch Activation In the Early Mesodermmentioning

confidence: 99%

Notch signaling patternsDrosophilamesodermal segments by regulating the bHLH transcription factortwist

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Baylies

2004

Development

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Notch is required for wingless signaling in the epidermis of Drosophila

Cited by 169 publications

References 53 publications

Analysis of the vestigial tail mutation demonstrates that Wnt-3a gene dosage regulates mouse axial development.

Analysis of the vestigial tail mutation demonstrates that Wnt-3a gene dosage regulates mouse axial development.

Delta is a ventral to dorsal signal complementary to Serrate, another Notch ligand, in Drosophila wing formation.

Notch signaling patternsDrosophilamesodermal segments by regulating the bHLH transcription factortwist

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