Notch-dependent binary fate choice regulates the Netrin pathway to control axon guidance of Drosophila visual projection neurons

Lowe, Scott; Ding, Andrew; Li, Xin

doi:10.1016/j.celrep.2023.112143

Cited by 4 publications

(1 citation statement)

References 45 publications

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“…In the future, one could leverage genetic manipulations that alter neuropil structure to assess how equivalent glia might alter their morphology and transcriptional profiles in distinct environments. For example, one could assess glial morphologies and transcriptional profiles while (i) altering neuronal diversity and composition by manipulating the temporal series in medulla neuroblasts [79][80][81][82], or by blocking lamina neuron differentiation [83,84]; or (ii) by modifying neuropil stratifications with genetic perturbations that disrupt neuronal targeting [85].…”

Section: Plos Biologymentioning

confidence: 99%

A Drosophila glial cell atlas reveals a mismatch between transcriptional and morphological diversity

Lago-Baldaia,

Cooper,

Seroka

et al. 2023

PLoS Biol

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Morphology is a defining feature of neuronal identity. Like neurons, glia display diverse morphologies, both across and within glial classes, but are also known to be morphologically plastic. Here, we explored the relationship between glial morphology and transcriptional signature using the Drosophila central nervous system (CNS), where glia are categorised into 5 main classes (outer and inner surface glia, cortex glia, ensheathing glia, and astrocytes), which show within-class morphological diversity. We analysed and validated single-cell RNA sequencing data of Drosophila glia in 2 well-characterised tissues from distinct developmental stages, containing distinct circuit types: the embryonic ventral nerve cord (VNC) (motor) and the adult optic lobes (sensory). Our analysis identified a new morphologically and transcriptionally distinct surface glial population in the VNC. However, many glial morphological categories could not be distinguished transcriptionally, and indeed, embryonic and adult astrocytes were transcriptionally analogous despite differences in developmental stage and circuit type. While we did detect extensive within-class transcriptomic diversity for optic lobe glia, this could be explained entirely by glial residence in the most superficial neuropil (lamina) and an associated enrichment for immune-related gene expression. In summary, we generated a single-cell transcriptomic atlas of glia in Drosophila, and our extensive in vivo validation revealed that glia exhibit more diversity at the morphological level than was detectable at the transcriptional level. This atlas will serve as a resource for the community to probe glial diversity and function.

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Section: Plos Biologymentioning

confidence: 99%

A Drosophila glial cell atlas reveals a mismatch between transcriptional and morphological diversity

Lago-Baldaia,

Cooper,

Seroka

et al. 2023

PLoS Biol

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Axon targeting of Drosophila medulla projection neurons requires diffusible Netrin and is coordinated with neuroblast temporal patterning

Lowe¹,

Ding²,

Li³

2023

Cell Reports

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Insight into the adaptive role of arachnid genome-wide duplication through chromosome-level genome assembly of the Western black widow spider

Miles,

Waterman,

Ayoub

et al. 2024

Journal of Heredity

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Although spiders are one of the most diverse groups of arthropods, the genetic architecture of their evolutionary adaptations is largely unknown. Specifically, ancient genome-wide duplication occurring during arachnid evolution ~450 mya resulted in a vast assembly of gene families, yet the extent to which selection has shaped this variation is understudied. To aid in comparative genome sequence analyses, we provide a chromosome-level genome of the Western black widow spider (Latrodectus hesperus)—a focus due to its silk properties, venom applications, and as a model for urban adaptation. We used long-read and Hi-C sequencing data, combined with transcriptomes, to assemble 14 chromosomes in a 1.46 Gb genome, with 38,393 genes annotated, and a BUSCO score of 95.3%. Our analyses identified high repetitive gene content and heterozygosity, consistent with other spider genomes, which has led to challenges in genome characterization. Our comparative evolutionary analyses of eight genomes available for species within the Araneoidea group (orb weavers and their descendants) identified 1,827 single-copy orthologs. Of these, 155 exhibit significant positive selection primarily associated with developmental genes, and with traits linked to sensory perception. These results support the hypothesis that several traits unique to spiders emerged from the adaptive evolution of ohnologs—or retained ancestrally duplicated genes—from ancient genome-wide duplication. These comparative spider genome analyses can serve as a model to understand how positive selection continually shapes ancestral duplications in generating novel traits today within and between diverse taxonomic groups.

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Notch-dependent binary fate choice regulates the Netrin pathway to control axon guidance of Drosophila visual projection neurons

Cited by 4 publications

References 45 publications

A Drosophila glial cell atlas reveals a mismatch between transcriptional and morphological diversity

A Drosophila glial cell atlas reveals a mismatch between transcriptional and morphological diversity

Axon targeting of Drosophila medulla projection neurons requires diffusible Netrin and is coordinated with neuroblast temporal patterning

Insight into the adaptive role of arachnid genome-wide duplication through chromosome-level genome assembly of the Western black widow spider

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