Notch activation promotes osteoblast mineralization by inhibition of apoptosis

Xu, Ying; Shu, Bing; Chelly, Marjorie; Morandi, Massimo; Barton, R. Shane; Shang, Xifu; Dong, Yufeng

doi:10.1002/jcp.26592

Cited by 22 publications

(15 citation statements)

References 26 publications

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“…But the possible mechanism was not shown. Recent studies have shown that BMP-Smad, Notch, and Wnt/β-Catenin signaling pathway play important roles in osteogenic differentiation of cells [ [61] , [62] , [63] , [64] , [65] ]. Therefore, in this study we hypothesized that the osteogenic effects of MCNTs were related to these signaling pathways.…”

Section: Discussionmentioning

confidence: 99%

The effect of carbon nanotubes on osteogenic functions of adipose-derived mesenchymal stem cells in vitro and bone formation in vivo compared with that of nano-hydroxyapatite and the possible mechanism

Feng

Cao

et al. 2021

Bioactive Materials

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It has been well recognized that the development and use of artificial materials with high osteogenic ability is one of the most promising means to replace bone grafting that has exhibited various negative effects. The biomimetic features and unique physiochemical properties of nanomaterials play important roles in stimulating cellular functions and guiding tissue regeneration. But efficacy degree of some nanomaterials to promote specific tissue formation is still not clear. We hereby comparatively studied the osteogenic ability of our treated multi-walled carbon nanotubes (MCNTs) and the main inorganic mineral component of natural bone, nano-hydroxyapatite (nHA) in the same system, and tried to tell the related mechanism. In vitro culture of human adipose-derived mesenchymal stem cells (HASCs) on the MCNTs and nHA demonstrated that although there was no significant difference in the cell adhesion amount between on the MCNTs and nHA, the cell attachment strength and proliferation on the MCNTs were better. Most importantly, the MCNTs could induce osteogenic differentiation of the HASCs better than the nHA, the possible mechanism of which was found to be that the MCNTs could activate Notch involved signaling pathways by concentrating more proteins, including specific bone-inducing ones. Moreover, the MCNTs could induce ectopic bone formation in vivo while the nHA could not, which might be because MCNTs could stimulate inducible cells in tissues to form inductive bone better than nHA by concentrating more proteins including specific bone-inducing ones secreted from M2 macrophages. Therefore, MCNTs might be more effective materials for accelerating bone formation even than nHA.

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Section: Discussionmentioning

confidence: 99%

The effect of carbon nanotubes on osteogenic functions of adipose-derived mesenchymal stem cells in vitro and bone formation in vivo compared with that of nano-hydroxyapatite and the possible mechanism

Feng

Cao

et al. 2021

Bioactive Materials

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“…The cell apoptosis was detected by the Annexin V FITC apoptosis detection kit (BD Biosciences, San Jose, CA, United States) as we described previously (Xu et al, 2018). Briefly, on Days 1, 4, and 7 after cell sheet culture, the cells were washed with PBS and trypsinized.…”

Section: Analysis Of Apoptotic Cellsmentioning

confidence: 99%

Wnt Signaling Inhibits High-Density Cell Sheet Culture Induced Mesenchymal Stromal Cell Aging by Targeting Cell Cycle Inhibitor p27

Tian

Tong

et al. 2020

Front. Bioeng. Biotechnol.

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Mesenchymal stromal cell senescence and apoptosis have been identified as critical molecular hallmarks in aging. In this study, we used stromal cell sheet culture as an in vitro model to study the progressive changes of cellular senescence, apoptosis and underlying mechanism in Wnt3a treated cells. Our results showed fresh bone marrow mesenchymal stromal cells (BMSCs) become senescent and undergo apoptosis with increased inflammatory profile and Reactive Oxygen Species (ROS) in high-density cell sheet cultures. The gene expression level of senescence related proteins and key regulators of apoptosis in cell sheet cultures was significantly increased in older BMSCs at Days 4 and 7 cultures compared with younger cells at Day 1 cultures. More importantly, Wnt signaling activation significantly reduced senescence in cell sheet cultures by direct regulation of cell cycle inhibitor p27. This study not only characterized the cellular and molecular features of aging stromal cells in short-term cell sheet cultures, but also identified the downstream target responsible for Wnt inhibition of cell senescence.

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“…Consistent with this finding, our study further showed that Notch ligand Jagged1-mediated primary osteoblast mineralization was enhanced through decreased cell apoptosis in a caspase 3edependent manner. 24 A similar effect also was observed in cultures of primary human bone marrowederived mesenchymal stem cells (MSCs). When human bone marrowederived MSCs were transduced with lentiviral vectors containing either human NICD1 or ligand jagged1, the cultures showed an enhanced mineralization.…”

Section: Tezuka Et Al 21 Examined the Role Of Notch Activation Inmentioning

confidence: 61%