Not every PRP‐gel is born equal Evaluation of growth factor availability for tissues through four PRP‐gel preparations: Fibrinet®, RegenPRP‐Kit®, Plateltex® and one manual procedure

Mazzucco, Laura; Balbo, Valeria; Cattana, Elena; Guaschino, Roberto; Borzini, P

doi:10.1111/j.1423-0410.2009.01188.x

Cited by 226 publications

(205 citation statements)

References 30 publications

(27 reference statements)

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“…Quantification of growth factor levels and the kinetics of release show similar concentrations of bFGF, VEGF, EGF and TGF-β1 and similar release kinetics with previous published results (Mazzucco et al, 2009). This is particularly remarkable considering that the method used to obtain the conditioned media was different in that the FIBRINET ® kit has been used to produce a matrix gel (Mazzucco et al, 2009) while in our study it has been used to produce a membrane. Further studies are needed to investigate whether the matrix gel and the membrane release growth factors with a similar kinetics.…”

Section: E Lucarelli Et Alsupporting

confidence: 58%

“…In addition, blood parametric analysis of the residual serum showed that only 0.9 % of platelets were left in the serum, indicating that 99.1% of the platelets were present in the PRFM (Table 1). Platelet recovery data from PRP obtained with the PRFM FIBRINET ® kit have been previously published from Leitner et al being 65.5% (Leitner et al, 2006;Mazzucco et al, 2009). Considering the platelet concentration factor represented by the PRFM, use of the www.ecmjournal.org E Lucarelli et al A new platelet-rich fibrin matrix PRFM kit produces a 210-fold higher concentration of platelets and fibrin when compared to the initial input whole blood volume.…”

Section: Prfm Descriptionmentioning

confidence: 99%

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A recently developed bifacial platelet-rich fibrin matrix

Lucarelli

Beretta

Dozza

et al. 2010

eCM

117

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Platelet-rich plasma (PRP) is used clinically in liquid or gel form to promote tissue repair. Because of the poor mechanical properties, conventional PRP is often difficult to handle when used in clinical settings and requires secure implantation in a specific site, otherwise when released growth factors could be washed out during an operation. In this study, we analyzed the end product of a recently developed commercially available system (FIBRINET ® ), which is a dense pliable, platelet-rich fibrin matrix (PRFM). We characterized the mechanical properties of PRFM and tested whether PRFM releases growth factors and whether released factors induce the proliferation of mesenchymal stem cells (MSC). Mechanical properties as well as platelet distribution were evaluated in PRFM. PRFM demonstrated robust mechanical properties, with a tear elastic modulus of 937.3 + 314.6 kPa, stress at a break of 1476.0 + 526.3 kPa, and an elongation at break of 146.3 + 33.8 %. PRFM maintained its mechanical properties throughout the testing process. Microscopic observations showed that the platelets were localized on one side of the matrix. Elevated levels of PDGF-AA, PDGF-AB, EGF, VEGF, bFGF and TGF-β1 were measured in the day 1-conditioned media (CM) of PRFM and growth factor levels decreased thereafter. BMP2 and BMP7 were not detectable. MSC culture media supplemented with 20% PRFM-CM stimulated MSC cell proliferation; at 24 and 48 hours the induction of the proliferation was significantly greater than the induction obtained with media supplemented with 20% foetal bovine serum. The present study shows that the production of a dense, physically robust PRFM made through high-speed centrifugation of intact platelets and fibrin in the absence of exogenous thrombin yields a potential tool for accelerating tissue repair.

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Section: E Lucarelli Et Alsupporting

confidence: 58%

Section: Prfm Descriptionmentioning

confidence: 99%

A recently developed bifacial platelet-rich fibrin matrix

Lucarelli

Beretta

Dozza

et al. 2010

eCM

117

View full text Add to dashboard Cite

show abstract

“…It is logical that if the processing of the sample damages or activates the platelets, the content of its granules would be released prematurely, and as a consequence, would be lost before being used. The study by Mazzucco et al (17) in 2009 further supports this hypothesis. It analyzes four different methods for obtaining platelet-rich plasma.…”

Section: References With Links To Crossref -Doisupporting

confidence: 75%

Quantification of growth factors by using a new system for obtaining platelet-rich plasma

Lorente-Pérez-Sierra

Ortega-Aranegui²,

Martín-Arés³

et al. 2011

Med Oral

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Objective: To verify the performance of a new method for obtaining platelet-rich plasma, while avoiding contamination of the sample during its processing. Study Design: Twenty healthy patients were selected, from whom 21 ml of blood was e�tracted. �e then proDesign: Twenty healthy patients were selected, from whom 21 ml of blood was e�tracted. �e then proesign: Twenty healthy patients were selected, from whom 21 ml of blood was e�tracted. �e then proceeded to study the platelets and growth factors in basal blood after centrifuging the sample by using a new closed system for obtaining platelet-rich plasma (PRP). Results: After centrifuging the blood sample, double the amount of platelets as that found in basal blood was obtained. Of the four growth factors analyzed, only the factor similar to insulin (IG�) contained the same concentration after the centrifuge process. The platelet-derived growth factor (PDG�) and the vascular growth factor (VG�) were multiplied by si� with respect to the basal values and disproportionately increased the levels of the transforming growth factor β (TGF-β). Conclusions: The new closed method for obtaining PRP, after avoiding contamination of the sample following its use, offers levels of platelet concentrate and growth factors necessary for regeneration.

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“…However, there are numerous complex variations among PRP preparation protocols including the starting number of platelets, the use of anticoagulants, the inclusion of leukocytes, and the use of activators [23][24][25][26][27] . The variation in PRP preparation contributes in part to the controversial outcomes both in animal and clinical studies 28 .…”

Section: Discussionmentioning

confidence: 99%

PRP as a New Approach to Prevent Infection: Preparation and <em>In vitro</em> Antimicrobial Properties of PRP

2013

JoVE

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Implant-associated infection is becoming more and more challenging to the healthcare industry worldwide due to increasing antibiotic resistance, transmission of antibiotic resistant bacteria between animals and humans, and the high cost of treating infections.In this study, we disclose a new strategy that may be effective in preventing implant-associated infection based on the potential antimicrobial properties of platelet-rich plasma (PRP). Due to its well-studied properties for promoting healing, PRP (a biological product) has been increasingly used for clinical applications including orthopaedic surgeries, periodontal and oral surgeries, maxillofacial surgeries, plastic surgeries, sports medicine, etc.PRP could be an advanced alternative to conventional antibiotic treatments in preventing implant-associated infections. The use of PRP may be advantageous compared to conventional antibiotic treatments since PRP is less likely to induce antibiotic resistance and PRP's antimicrobial and healing-promoting properties may have a synergistic effect on infection prevention. It is well known that pathogens and human cells are racing for implant surfaces, and PRP's properties of promoting healing could improve human cell attachment thereby reducing the odds for infection. In addition, PRP is inherently biocompatible, and safe and free from the risk of transmissible diseases.For our study, we have selected several clinical bacterial strains that are commonly found in orthopaedic infections and examined whether PRP has in vitro antimicrobial properties against these bacteria. We have prepared PRP using a twice centrifugation approach which allows the same platelet concentration to be obtained for all samples. We have achieved consistent antimicrobial findings and found that PRP has strong in vitro antimicrobial properties against bacteria like methicillin-sensitive and methicillin-resistant Staphylococcus aureus, Group A Streptococcus, and Neisseria gonorrhoeae. Therefore, the use of PRP may have the potential to prevent infection and to reduce the need for costly post-operative treatment of implant-associated infections. Video LinkThe video component of this article can be found at

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Not every PRP‐gel is born equal Evaluation of growth factor availability for tissues through four PRP‐gel preparations: Fibrinet®, RegenPRP‐Kit®, Plateltex® and one manual procedure

Abstract: Similar methods for platelet gel preparation revealed different performances concerning growth factor recovery and the kinetics of its release from the gel. It is unclear whether these noticeable differences are important for clinical management.

Cited by 226 publications

References 30 publications

A recently developed bifacial platelet-rich fibrin matrix

A recently developed bifacial platelet-rich fibrin matrix

Quantification of growth factors by using a new system for obtaining platelet-rich plasma

PRP as a New Approach to Prevent Infection: Preparation and <em>In vitro</em> Antimicrobial Properties of PRP

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