Not All Binding Sites Are Equal: Site Determination and Folding State Analysis of Gas‐Phase Protein‐Metallodrug Adducts
Liam Eade,
Matthew P. Sullivan,
Timothy M. Allison
et al.
Abstract:Modern approaches in metallodrug research focus on compounds that bind protein targets rather than DNA. However, the identification of protein targets and binding sites is challenging. Using intact mass spectrometry and proteomics, we investigated the binding of antimetastatic agent RAPTA‐C to the model proteins ubiquitin, cytochrome c, lysozyme and myoglobin. Binding to cytochrome c and lysozyme was negligible. However, ubiquitin bound up to three Ru moieties, two of which were localized at Met1 and His68 as … Show more
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