Nosocomial transmission of disease caused by nontypeable strains of haemophilus influenzae

Goetz, Matthew Bidwell; O’Brien, Helen; Musser, James M.; Ward, Joel I.

doi:10.1016/0002-9343(94)90064-7

Cited by 23 publications

(11 citation statements)

References 34 publications

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“…At the nursing home, 8 isolates from ill individuals or their asymptomatic roommates had a single outer membrane protein profile while isolates from patients at an adjacent acute care facility all had different profiles, suggesting horizontal transmission among nursing home residents. 4 Similarly, Loos et al used DNA restriction fingerprinting to demonstrate that identical strains were found in the nasopharynx and middle ear fluid of a child with acute otitis media; and when 3 siblings were cultured, they found similar strains of NTHi, suggesting transmission within the household setting. 20 Watanabe et al evaluated displaced persons in evacuation camps following the 2004 Sri Lankan tsunami and found 20 individuals colonized with NTHi.…”

Section: Discussionmentioning

confidence: 99%

“…4-5 To further understand NTHi colonization and transmission, we performed a study with the following objectives: (1) to determine the prevalence of NTHi carriage in the respiratory tract of infants and their primary caregivers, (2) to examine the frequency of NTHi co-colonization across infant-primary caregiver dyads, and (3) to characterize the genetic diversity of NTHi isolates present in the respiratory tract of infants and their primary caregivers by multilocus sequence typing (MLST).…”

Section: Introductionmentioning

confidence: 99%

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Prevalence and Genetic Diversity of Nontypeable Haemophilus influenzae in the Respiratory Tract of Infants and Primary Caregivers

Schumacher¹,

Marchant²,

Loughlin³

et al. 2012

Pediatric Infectious Disease Journal

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BACKGROUND Nontypeable Haemophilus influenzae (NTHi) causes otitis media, sinusitis, and likely lower respiratory tract infections in children. Colonization, strain diversity, transmission, and antimicrobial susceptibility have implications for both children and their caregivers. METHODS For 13 months, we conducted a cross-sectional study of NTHi colonization. 273 infants and children aged 2 to 26 months old and their primary caregivers had upper respiratory tract cultures performed. NTHi isolates were characterized by multilocus sequence typing (MLST) and antibiotic resistance was examined. RESULTS Of the 273 infants, 44 (16.1%) were colonized with NTHi. Prevalence of NTHi varied from 14% in infants less than 6 months of age to 32% in infants 19-26 months of age (p=0.003). NTHi colonized infants were more likely to attend daycare (30% vs. 11%), have a recent respiratory infection (68% vs. 38%), recent antibiotic use (27% vs. 9%), and caregiver reported asthma (11% vs. 1%) compared with other infants (p<0.001). Of the 44 infants colonized with NTHi, we identified 33 different MLSTs. Nine (20.5%) of the 44 infant-primary caregiver dyads were colonized with NTHi and 7/9 shared identical NTHi strains. We also found beta-lactamase negative NTHi with minimum inhibitory concentrations >2 μg/mL for amoxicillin and beta-lactamase positive NTHi with minimum inhibitory concentrations >2 μg/mL for amoxicillin clavulanate. CONCLUSIONS We found substantial diversity by MLST analysis among NTHi isolates from this community. Infant-primary caregiver dyads usually carried the same strain of NTHi, suggesting that infant-primary caregiver transmission is occurring.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Prevalence and Genetic Diversity of Nontypeable Haemophilus influenzae in the Respiratory Tract of Infants and Primary Caregivers

Schumacher¹,

Marchant²,

Loughlin³

et al. 2012

Pediatric Infectious Disease Journal

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“…Nontypeable H. influenzae causes conjunctivitis in children, the elderly, health care workers, and immunocompromised hosts. Person-to-person transmission, particularly in day care centers and nursing homes, plays an important role in the spread of infection in these settings (14,47).…”

Section: Clinical Manifestations Of H Influenzae Infectionsmentioning

confidence: 99%

Haemophilus influenzae Infections in the H. influenzae Type b Conjugate Vaccine Era

2011

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The widespread use of Haemophilus influenzae type b (Hib) conjugate vaccines has nearly eradicated invasive Hib disease where the vaccines are used. This success was accompanied by a shift in capsular serotypes of invasive H. influenzae disease, with nontypeable strains replacing type b strains as the most common bloodstream isolate, but there is no convincing evidence of a true increase in the incidence of non-serotype b invasive infections. H. influenzae causes predominantly mucosal infections. The introduction of vaccines for otitis media and global shifts in antimicrobial susceptibility emphasize the importance of continued surveillance of H. influenzae colonization and disease patterns.

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“…Non-capsulate Haemophilus influenzae (NCHI) is associated with respiratory tract infections and outbreaks of infection have been reported [ 1,2]. NCHI is now also recognised as an important cause of invasive disease [3,4].…”

Section: Introductionmentioning

confidence: 99%

Characterisation of non-capsulate Haemophilus influenzae by repetitive extragenic palindromic (REP)-PCR

Jordens

1998

Journal of Medical Microbiology

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The use of repetitive extragenic palindromic (REP)-PCR to characterise non-capsulateHaemophilus inyuenzae (NCHI) for epidemiological studies was validated by application to four outbreak-associated and three epidemiologically unrelated NCHI strains of the same phenotype which had been well characterised by other methods. The REP-PCR patterns were reproducible and showed the unrelated isolates to be distinguishable from each other, whereas the outbreak-associated isolates were indistinguishable. The results were concordant with those from outer-membrane protein enriched profiles, ribotyping and randomly amplified polymorphic DNA analysis. When applied to six further isolates from two different suspected outbreaks, rapid results were obtained from boiled supernates prepared from one colony and indicated that the isolates in question were not related. REP-PCR provides a rapid method of strain characterisation suitable for NCHI, which is ideal for use in conjunction with other methods.

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Nosocomial transmission of disease caused by nontypeable strains of haemophilus influenzae

Cited by 23 publications

References 34 publications

Prevalence and Genetic Diversity of Nontypeable Haemophilus influenzae in the Respiratory Tract of Infants and Primary Caregivers

Prevalence and Genetic Diversity of Nontypeable Haemophilus influenzae in the Respiratory Tract of Infants and Primary Caregivers

Haemophilus influenzae Infections in the H. influenzae Type b Conjugate Vaccine Era

Characterisation of non-capsulate Haemophilus influenzae by repetitive extragenic palindromic (REP)-PCR

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