NOSA, an Analytical Toolbox for Multicellular Optical Electrophysiology

Oltmanns, Sebastian; Abben, Frauke Sophie; Ender, Anatoli; Aimon, Sophie; Kovacs, Richard J.; Sigrist, Stephan J.; Storace, Douglas A.; Geiger, Jörg R. P.; Raccuglia, Davide

doi:10.3389/fnins.2020.00712

Cited by 6 publications

(2 citation statements)

References 51 publications

(70 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Fast recordings were taken at a speed of 30 Hz with a resonant scan head using mesc software (Femtonics). Analysis was performed using NOSA software (neuro-optical signal analysis) 112 and a customized R script. ROIs were manually drawn for analysis.…”

Section: Methodsmentioning

confidence: 99%

Mating proximity blinds threat perception.

Cazale Debat,

Scheunemann,

Day

et al. 2024

Preprint

View full text Add to dashboard Cite

Romantic engagement can bias sensory perception. This 'love blindness' reflects a common behavioral principle across organisms: favoring pursuit of a coveted reward over potential risks. In the case of animal courtship, such sensory biases may support reproductive success but can also expose individuals to danger, such as predation. How do neural networks balance the trade-off between risk and reward? Here, we discover a dopamine-governed filter mechanism in male Drosophila that reduces threat perception as courtship progresses. We show that during early courtship stages, threat-activated visual neurons inhibit central courtship nodes via specific serotonergic neurons. This serotonergic inhibition prompts flies to abort courtship when they see imminent danger. However, as flies advance in the courtship process, the dopaminergic filter system reduces visual threat responses, shifting the balance from survival to mating. By recording neural activity from males as they approach mating, we demonstrate that progress in courtship is registered as dopaminergic activity levels ramping up. This dopamine signaling inhibits the visual threat detection pathway via Dop2R receptors, allowing male flies to focus on courtship when they are close to copulation. Thus, dopamine signaling biases sensory perception based on perceived goal proximity, in order to prioritize between competing behaviors.

show abstract

Section: Methodsmentioning

confidence: 99%

Mating proximity blinds threat perception.

Cazale Debat,

Scheunemann,

Day

et al. 2024

Preprint

View full text Add to dashboard Cite

show abstract

“…We used the publicly available implementations of PMD ( https://github.com/ikinsella/trefide ), DeepCAD-RT ( https://github.com/cabooster/DeepCAD-RT ), NOSA 58 ( https://github.com/DavideR2020/NOSA ) and Volpy 59 ( https://github.com/flatironinstitute/CaImAn ).…”

Section: Methodsmentioning

confidence: 99%

Statistically unbiased prediction enables accurate denoising of voltage imaging data

Eom,

Han,

Park

et al. 2023

Nat Methods

View full text Add to dashboard Cite

Here we report SUPPORT (statistically unbiased prediction utilizing spatiotemporal information in imaging data), a self-supervised learning method for removing Poisson–Gaussian noise in voltage imaging data. SUPPORT is based on the insight that a pixel value in voltage imaging data is highly dependent on its spatiotemporal neighboring pixels, even when its temporally adjacent frames alone do not provide useful information for statistical prediction. Such dependency is captured and used by a convolutional neural network with a spatiotemporal blind spot to accurately denoise voltage imaging data in which the existence of the action potential in a time frame cannot be inferred by the information in other frames. Through simulations and experiments, we show that SUPPORT enables precise denoising of voltage imaging data and other types of microscopy image while preserving the underlying dynamics within the scene.

show abstract

Mating proximity blinds threat perception

Cazalé-Debat,

Scheunemann,

Day

et al. 2024

Nature

View full text Add to dashboard Cite

Romantic engagement can bias sensory perception. This ‘love blindness’ reflects a common behavioural principle across organisms: favouring pursuit of a coveted reward over potential risks1. In the case of animal courtship, such sensory biases may support reproductive success but can also expose individuals to danger, such as predation2,3. However, how neural networks balance the trade-off between risk and reward is unknown. Here we discover a dopamine-governed filter mechanism in male Drosophila that reduces threat perception as courtship progresses. We show that during early courtship stages, threat-activated visual neurons inhibit central courtship nodes via specific serotonergic neurons. This serotonergic inhibition prompts flies to abort courtship when they see imminent danger. However, as flies advance in the courtship process, the dopaminergic filter system reduces visual threat responses, shifting the balance from survival to mating. By recording neural activity from males as they approach mating, we demonstrate that progress in courtship is registered as dopaminergic activity levels ramping up. This dopamine signalling inhibits the visual threat detection pathway via Dop2R receptors, allowing male flies to focus on courtship when they are close to copulation. Thus, dopamine signalling biases sensory perception based on perceived goal proximity, to prioritize between competing behaviours.

show abstract

NOSA, an Analytical Toolbox for Multicellular Optical Electrophysiology

Cited by 6 publications

References 51 publications

Mating proximity blinds threat perception.

Mating proximity blinds threat perception.

Statistically unbiased prediction enables accurate denoising of voltage imaging data

Mating proximity blinds threat perception

Contact Info

Product

Resources

About