Norwegian patients and retail chicken meat share cephalosporin-resistant Escherichia coli and IncK/ bla CMY-2 resistance plasmids

Berg, Einar Sverre; Wester, Astrid Lousie; Ahrenfeldt, Johanne; Mo, Solveig Sølverød; Slettemeås, Jannice Schau; Steinbakk, Martin; Samuelsen, Ørjan; Grude, Nils; Simonsen, Gunnar Skov; Löhr, Iren Høyland; Jørgensen, Silje Bakken; Tofteland, Ståle; Lund, Ole; Dahle, Ulf R.; Sunde, Marianne

doi:10.1016/j.cmi.2016.12.035

Cited by 53 publications

(42 citation statements)

References 26 publications

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“…Such E. coli strains carrying bla CMY-2 -IncK2 plasmids, including strains belonging to ST38, were also associated with broiler production in other European countries (17,18). Furthermore, a recent Norwegian report strongly supports the hypothesis that clonal transfer of these ST38 E. coli strains from chicken meat to humans may occur (19). Thus, the additional acquisition of mcr-1 in hyperepidemic extended-spectrum ␤-lactamase (ESBL)-producing ST38 E. coli (e.g., strain Mbl488 found in the present analysis) is concerning.…”

Section: Emergence Of Mcr-1-carrying Inck2 Plasmidsmentioning

confidence: 71%

“…Some of the plasmids were highly similar to others previously found in diverse E. coli lineages from different sources, suggesting horizontal spread. Nevertheless, direct colonization with mcr-1-carrying hyperepidemic E. coli clones from other reservoirs (e.g., food animals) cannot be excluded, as already suggested for ST38 E. coli carrying bla CMY-2 -IncK2 plasmids (17)(18)(19). Therefore, adequate measures should be taken, particularly in broiler production, to limit the acquisition of the mcr-1 gene and further spread of such clones.…”

Section: Emergence Of Mcr-1-carrying Inck2 Plasmidsmentioning

confidence: 99%

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Heterogeneous Genetic Location of mcr-1 in Colistin-Resistant Escherichia coli Isolates from Humans and Retail Chicken Meat in Switzerland: Emergence of mcr-1 -Carrying IncK2 Plasmids

Donà

Bernasconi

Pires

et al. 2017

Antimicrob Agents Chemother

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We characterized the genetic environment of mcr-1 in colistin-resistant Escherichia coli strains isolated in Switzerland during 2014 to 2016 from humans (n ϭ 3) and chicken meat (n ϭ 6). Whole-genome and plasmid sequencing identified the mcr-1 gene integrated in IncX4 (of which, one strain carried the mcr-1.2 variant), IncI2, IncHI2, and novel IncK2 plasmids (overall, n ϭ 7), as well as in the bacterial chromosome (n ϭ 2) in single or duplicate copies. Our study supports the easy mobilization of mcr-1 across diverse genetic locations.

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Section: Emergence Of Mcr-1-carrying Inck2 Plasmidsmentioning

confidence: 71%

Section: Emergence Of Mcr-1-carrying Inck2 Plasmidsmentioning

confidence: 99%

Heterogeneous Genetic Location of mcr-1 in Colistin-Resistant Escherichia coli Isolates from Humans and Retail Chicken Meat in Switzerland: Emergence of mcr-1 -Carrying IncK2 Plasmids

Donà

Bernasconi

Pires

et al. 2017

Antimicrob Agents Chemother

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“…The plasmid composition and location of the mcr‐1 gene in IncX4 resembles an isolate that has been reported from a pig in Austria . Recent studies from Sweden and Norway have identified close genetic relatedness of E. coli isolates carrying the bla CMY‐2 gene both from human samples and retail poultry meat, which suggests a possible common source. The source of the Finnish mcr‐1 E. coli isolate, however, remains unknown.…”

Section: Discussionmentioning

confidence: 61%

The first human report of mobile colistin resistance gene, mcr‐1, in Finland

Gröndahl-Yli-Hannuksela

Lönnqvist

Kallonen

et al. 2018

APMIS

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Colistin resistance mediated by mobile mcr-1 gene has raised concern during the last years. After steep increase in mcr-1 reports, other mcr-gene variants (mcr-2 to mcr-5) have been revealed as well. In 2016, a clinical study was conducted on asymptomatic stool carriage of extended spectrum beta-lactamase (ESBL) producing Escherichia coli and Klebsiella pneumoniae among Finnish adults. All suspected ESBL producing bacterial isolates were first tested by phenotypic ESBL-confirmation methods, and then further analyzed with whole genome sequencing to identify the resistance genes. We found one study subject carrying a colistin resistant E. coli with a transferrable mcr-1 gene. This multi-drug resistant isolate, although initially suspected to be an ESBL producer, did not carry any ESBL genes, but was proven to carry several other resistance genes by using whole genome sequencing. Sequence type was ST93. The mcr-1 gene was connected to IncX4 plasmid which suggests that the colistin resistance gene locates in the respective plasmid. Here, we report the finding of a mcr-1 harboring human E. coli isolate from Finland. Clinical antimicrobial resistance (AMR) rates are low in Finland, and mobile colistin resistance has not been reported previously. This highlights the importance of AMR surveillance also in populations with low levels of resistance.

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“…This result suggests that the transmission of cefoxitin-resistant S. enterica serovar Heidelberg strains among human, abattoir poultry, and retail poultry sources may happen. The clonal transmission of opportunistic pathogens, such as Escherichia coli, Klebsiella pneumoniae, and Clostridium difficile, was also detected [29][30][31][32][33][34][35][36][37][38]. The whole genome sequencing of C. difficile strains, which are a commensal organism and a pathogen in both human and animal [39], showed that C. difficile isolates with identical (no SNP differences) and almost identical (less than two SNP differences) are detected in both farmers and pigs in the Netherlands [34].…”

Section: Observational Evidences For the Transmission Of Antibiotic Rmentioning

confidence: 99%

The need for efforts to obtain high quality evidence in a one health approach

Lee¹,

Lee²,

Park³

et al. 2018

biomedicalresearch

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The progressive increase of antibiotic resistance poses an alarming threat on public health worldwide. Although antibiotic resistance is a problem of human health, a one health approach to tackling antibiotic resistance is required, due to the effect of animal and environment on human health. To exactly estimate the impact of animal and environment on the spread of antibiotic resistance in human, scientific evidence with high quality is required. Molecular analysis of antibiotic-resistant bacteria through high-resolution, such as whole-genome sequencing, is suitable for obtaining credible evidence in examining the transmission of strains or genetic mobile elements responsible for antibiotic resistance among human, animal, and environment. In this paper, we discuss many observational studies using the whole-genome sequencing on various isolates collected from human, animal, and environment. These analyses suggest that more extensive surveillance based on whole-genome sequencing is required to accurately assess the potential threat of animal and environment on the spread of antibiotic resistance in human and that the effort to overcome various limitations in obtaining higher quality evidence is required.

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Norwegian patients and retail chicken meat share cephalosporin-resistant Escherichia coli and IncK/ bla CMY-2 resistance plasmids

Cited by 53 publications

References 26 publications

Heterogeneous Genetic Location of mcr-1 in Colistin-Resistant Escherichia coli Isolates from Humans and Retail Chicken Meat in Switzerland: Emergence of mcr-1 -Carrying IncK2 Plasmids

Heterogeneous Genetic Location of mcr-1 in Colistin-Resistant Escherichia coli Isolates from Humans and Retail Chicken Meat in Switzerland: Emergence of mcr-1 -Carrying IncK2 Plasmids

The first human report of mobile colistin resistance gene, mcr‐1, in Finland

The need for efforts to obtain high quality evidence in a one health approach

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