Norovirus outbreak in a cruise ship along the Brazilian coast, March 2011

Gabbay, Yvone Benchimol; Siqueira, Jones Anderson Monteiro; Lima, Ian Carlos Gomes de; Teixeira, Dielle Monteiro; Aragão, Glicélia Cruz; Barbagelata, Luana Soares; Oliveira, Darleise de Souza; Garcia, Carla Gisele Ribeiro; Galindo, Daniele de Barros; Mascarenhas, Joana D’Arc Pereira; Linhares, Alexandre da Costa

doi:10.5123/s2176-62232014000100005

Cited by 3 publications

(1 citation statement)

References 33 publications

(31 reference statements)

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“…Other studies have demonstrated the presence of NoV infection in inpatients, outbreaks, and sporadic cases of diarrhea in children living in the Amazon Region. Among these we can highlight: NoV infections in Belém (inpatients: 36.5%), NoV in cruise ships (outbreak: 3%), outbreak of acute diarrheal disease in Paraíso do Tocantins (4%), infections among hospitalized children in Porto Velho (7.8%), detection of a recombinant GII.7/GII.20 strain in an African‐descendant community (19.7%), and NoV in high prevalence among sporadic cases in Manaus (35.2%) . NoV was previously reported in Rio Branco, Acre, in two situations; detection of genotypes GII.16 and GII.17 in a study involving Brazil states, as well as the first description of the GII.4 Sydney variant in Brazil …”

Section: Introductionmentioning

confidence: 99%

Epidemiological and molecular investigation of norovirus and astrovirus infections in Rio Branco, Acre, Northern Brazil: A retrospective study

Bitencurt

Siqueira

Medeiros

et al. 2019

Journal of Medical Virology

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Norovirus (NoV) is a major cause of nonbacterial acute gastroenteritis (AGE) outbreaks worldwide, with infections reported in semiclosed environments, particularly in hospitals and nursing homes. Astrovirus (HAstV) is prevalent worldwide, especially in developing countries. We aimed to determine the prevalence, spatial distribution, and genetic diversity of NoV and HAstV in children under 5 years of age in Rio Branco city, Acre State, Amazon Region, Brazil. Stool samples from children with (n = 240) and without (n = 248) AGE were collected from January to December 2012 from seven neighborhoods. The overall NoV prevalence was 12.3% (60 of 488); representing 15.8% (38 of 240) of the symptomatic samples and 8.9% (22 of 248) of the controls. HAstVs infection was observed in 4.7% (23 of 488) of the samples tested, 6.2% (15 of 240) of AGE cases, and 2.4% (6 of 248) of the controls (plus two without information about feces consistency). Infections were found in all age groups with higher frequency in children less than two years of age, for both viruses. NoV was detected in all neighborhoods, with a higher concentration in the fourth (30%; 18 of 60). NoV nucleotide sequencing performed in 86.7% (52 of 60) of the positive samples showed the circulation of the strains GII.4 (57.7%; 30 of 52), GIIPe/GII.4 (19.2%; 10 of 52), GII.7, GII.Pg/GII.1, and GII.Pc (3.8%; 2 of 52 for each), GII.6 and GII.Pg (1.9%; 1 of 52 for each), and GI.3 (7.7%; 4 of 52). Three GII.4 variants were detected: Den Haag_2006b (n = 1), New Orleans_2009 (n = 1), and Sydney_2012 (n = 14). HAstV types HAstV‐1a (81.8%; 9 of 11) and HAstV‐2c (18.2%; 2 of 11) were observed in the 47.8% (11 of 23) of characterized samples. This is the first data obtained in Acre State regarding the prevalence of these viruses and provides epidemiological and molecular information for a better understanding of their role among children with and without AGE.

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Section: Introductionmentioning

confidence: 99%

Epidemiological and molecular investigation of norovirus and astrovirus infections in Rio Branco, Acre, Northern Brazil: A retrospective study

Bitencurt

Siqueira

Medeiros

et al. 2019

Journal of Medical Virology

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Norovirus GII.Pe Genotype: Tracking a Foodborne Outbreak on a Cruise Ship Through Molecular Epidemiology, Brazil, 2014

et al. 2016

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Norovirus (NoV) is recognized as the most common cause of foodborne outbreaks. In 2014, an outbreak of acute gastroenteritis occurred on a cruise ship in Brazil, and NoV became the suspected etiology. Here we present the molecular identification of the NoV strains and the use of sequence analysis to determine modes of virus transmission. Food (cream cheese, tuna salad, grilled fish, orange mousse, and vegetables soup) and clinical samples were analyzed by ELISA, conventional RT-PCR, qRT-PCR, and sequencing. Genogroup GII NoV was identified by ELISA and conventional RT-PCR in fecal samples from 5 of 12 patients tested (41.7%), and in the orange mousse food sample by conventional RT-PCR and qRT-PCR. Two fecal GII NoV samples and the orange mousse GII NoV sample were successfully genotyped as GII.Pe (ORF 1), revealed 98.0-98.8% identities among them, and shared phylogenetically distinct cluster. Establishing the source of a NoV outbreak can be a challenging task. In this report, the molecular analysis of the partial RdRp NoV gene provided a powerful tool for genotyping (GII.Pe) and tracking of outbreak-related samples. In addition, the same fast and simple extraction methods applied to clinical samples could be successfully used for complex food matrices, and have the potential to be introduced in routine laboratories for screening foods for presence of NoV.

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Norovírus em alimentos

Luz

Miagostovich

2017

Visa em Debate

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Norovirus outbreak in a cruise ship along the Brazilian coast, March 2011

Cited by 3 publications

References 33 publications

Epidemiological and molecular investigation of norovirus and astrovirus infections in Rio Branco, Acre, Northern Brazil: A retrospective study

Epidemiological and molecular investigation of norovirus and astrovirus infections in Rio Branco, Acre, Northern Brazil: A retrospective study

Norovirus GII.Pe Genotype: Tracking a Foodborne Outbreak on a Cruise Ship Through Molecular Epidemiology, Brazil, 2014

Norovírus em alimentos

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