Norovirus disease among older adults

Tsai, Helen; Yune, Philip S.; Rao, Mana

doi:10.1177/20499361221136760

Cited by 3 publications

(1 citation statement)

References 87 publications

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“…3,4,5 Although many people have been affected by this disease, antiviral agents and vaccines are still in the development stage, with supportive care being the primary treatment. 6 Therefore, surveillance https://doi.org/10.26434/chemrxiv-2024-5fqsq ORCID: https://orcid.org/0000-0002-4678-6147 Content not peer-reviewed by ChemRxiv. License: CC BY-NC-ND 4.0 and disinfection are currently the only effective methods for preventing the spread of norovirus infections.…”

Section: Introductionmentioning

confidence: 99%

Establishment and characterization of noro-VLP measurement by digital ELISA

Hasegawa,

Adachi,

Saikusa

et al. 2024

Preprint

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Highly sensitive viral analytical techniques are essential tools for preventing the spread of infections. In this study, we established a digital enzyme-linked immunosorbent assay (ELISA) system to quantify norovirus proteins with high sensitivity. We used norovirus-like particles (noro-VLPs) as a surrogate for norovirus and constructed two digital ELISA systems using two different antibody pairs. The quantitative performance of the noro-VLP measurement using each digital ELISA system was evaluated. Both assay systems exhibited high sensitivity, good linearity, and high stability. The first system exhibited a limit of detection (LOD) of 87 pg/mL, correlation coefficient (R2) of 0.9984, inter-assay variation of 5.5 %, and intra-assay variation of 5.2 %. The second system exhibited an LOD of 19 pg/mL, R2 of 0.9984, inter-assay variation of 4.5 %, and intra-assay variation of 2.5 %. Comparison of the two systems using the same calibrant for unpurified and fractionated noro-VLPs revealed that the quantitative values for unpurified noro-VLPs were the same, whereas those for fractionated noro-VLPs were dramatically different. Our findings indicate that the reactivity to various components in the noro-VLP solution was altered depending on the different antibodies. Furthermore, our study highlights the importance of using appropriate calibrants, which contain the same ratio of components as the noro-VLP analyte, to afford accurate measurements.

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Section: Introductionmentioning

confidence: 99%

Establishment and characterization of noro-VLP measurement by digital ELISA

Hasegawa,

Adachi,

Saikusa

et al. 2024

Preprint

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Glycan-lectin interactions powered biosensors for the instant screening of viruses and beyond

Li,

Ji,

Wang

et al. 2023

TrAC Trends in Analytical Chemistry

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Prevalence of Enteric Pathogens and Antibiotic Resistance: Results of a Six-Year Active Surveillance Study on Patients Admitted to a Teaching Hospital

Marascio,

Pavia,

Brescia

et al. 2024

Antibiotics

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Background: Acute Infectious Diarrhea (AID) and the short- and long-term complications associated with it are major causes of hospitalization worldwide. In Italy, due to a lack of robust surveillance programs, only limited data has been collected on their prevalence and circulation. This study aims to evaluate the resistance pattern of enteric pathogens and their epidemiological trends over a six-year period. Methods: This cross-sectional retrospective study was conducted from January 2018 to December 2023. Stool samples were analyzed during routine diagnosis with culture methods, syndromic molecular tests, and enzyme immunoassay. Results: Bacteria were the most isolated enteric pathogens (62.2%), followed by fungi (29.0%), viruses (8.2%), and parasites (0.6%). Most bacteria were isolated from outpatients (29.5%) and from patients in the Oncology ward (26.2%). The most prevalent target was EPEC (11.1%), followed by C. difficile toxin A/B-producing strains (8.3%), C. jejuni (2.5%), and S. enterica, (1%.). Norovirus and Candida spp. were the most prevalent in pediatric patients (6.5% and 39.6%, respectively). In the last years, enteric pathogens have been a frequent cause of infections characterized by a problematic resistance to common antimicrobials. In our study, S. enterica showed resistance to amikacin, gentamicin, ampicillin, levofloxacin, and ciprofloxacin. C. jejuni was susceptible to all tested drugs. Conclusion: Timely notification of gastroenteric infections is crucial in identifying potential outbreak sources and ensuring strict adherence to food safety and hygiene practices, so as to protect the most vulnerable populations. The present study offers insights into the epidemiological characteristics and the antibiotic susceptibility of the main enteric AID pathogens in order to implement infection control measures in health care settings.

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Norovirus disease among older adults

Cited by 3 publications

References 87 publications

Establishment and characterization of noro-VLP measurement by digital ELISA

Establishment and characterization of noro-VLP measurement by digital ELISA

Glycan-lectin interactions powered biosensors for the instant screening of viruses and beyond

Prevalence of Enteric Pathogens and Antibiotic Resistance: Results of a Six-Year Active Surveillance Study on Patients Admitted to a Teaching Hospital

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