2018
DOI: 10.1097/tp.0000000000002245
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Normothermic Ex Vivo Kidney Perfusion Reduces Warm Ischemic Injury of Porcine Kidney Grafts Retrieved After Circulatory Death

Abstract: In this porcine model of donation after circulatory death kidney transplantation NEVKP reduced kidney injury and improved graft function when compared with no-preservation. The results suggest that NEVKP does not cause additional damage to grafts during the preservation period, but may reverse the negative effects of warm ischemic insult itself and promotes regeneration.

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Cited by 35 publications
(38 citation statements)
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“…Two biopsies with insufficient protein yield (<100 g) to generate comparable results to the remaining biopsies were excluded. As previously reported by our group, 42 NEVKP-preserved grafts demonstrated superior kidney function after heterotopic autotransplantation compared to SCS-preserved grafts, with significantly lower serum creatinine (SCr) post-operatively in the NEVKP group compared to the SCS ( Figure 1B)(F-test, p<2.23x10 -15 ). Light microscopy demonstrated normal histology at baseline, with mild tubular injury in both groups at 30minutes post-reperfusion, slightly more prominent in SCS ( Figure 1C), as previously reported in this model.…”
Section: Proteomic Analysis Of Nevkp and Scs Biopsiessupporting
confidence: 78%
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“…Two biopsies with insufficient protein yield (<100 g) to generate comparable results to the remaining biopsies were excluded. As previously reported by our group, 42 NEVKP-preserved grafts demonstrated superior kidney function after heterotopic autotransplantation compared to SCS-preserved grafts, with significantly lower serum creatinine (SCr) post-operatively in the NEVKP group compared to the SCS ( Figure 1B)(F-test, p<2.23x10 -15 ). Light microscopy demonstrated normal histology at baseline, with mild tubular injury in both groups at 30minutes post-reperfusion, slightly more prominent in SCS ( Figure 1C), as previously reported in this model.…”
Section: Proteomic Analysis Of Nevkp and Scs Biopsiessupporting
confidence: 78%
“…Light microscopy demonstrated normal histology at baseline, with mild tubular injury in both groups at 30minutes post-reperfusion, slightly more prominent in SCS ( Figure 1C), as previously reported in this model. 42 Tubular injury and dilatation was evident at POD3, and was more severe in SCS-treated kidneys ( Figure 1C). Figure 1D.…”
Section: Proteomic Analysis Of Nevkp and Scs Biopsiesmentioning
confidence: 96%
“…Encouraging data about long‐term NMP in the kidney have been reported by Selzner's group at the University of Toronto using an experimental DBD and DCD porcine model. They demonstrated that longer periods of up to 16‐h of NMP are superior to both SCS alone and short‐duration NMP in terms of both tubular injury and post‐transplant organ function .…”
Section: Perfusion and Re‐conditioning Methods For Abdominal Organsmentioning
confidence: 99%
“…While technically more complex, its advantages include the reduction of cold ischemia, as well as increased metabolic activity, while providing options to assess renal function and modify/repair grafts prior to transplantation. In the second millennium, this technology was widely tested in Europe, Canada, and United States [39], demonstrating better function and regeneration compared to CS [40] or HMP [41]. Hamar et al demonstrated that a porcine kidney exposed to 30 min of warm ischemia (WI) + 8 h EVNP had lower daily serum creatinine levels and better regeneration markers compared to grafts exposed to WI + 8 h CS [40].…”
Section: Alternative Preservation Temperaturesmentioning
confidence: 99%
“…In the second millennium, this technology was widely tested in Europe, Canada, and United States [39], demonstrating better function and regeneration compared to CS [40] or HMP [41]. Hamar et al demonstrated that a porcine kidney exposed to 30 min of warm ischemia (WI) + 8 h EVNP had lower daily serum creatinine levels and better regeneration markers compared to grafts exposed to WI + 8 h CS [40]. Metcalfe et al observed superior graft function with porcine kidneys at 38 • C during 16 h of autologous blood perfusion versus HMP [41].…”
Section: Alternative Preservation Temperaturesmentioning
confidence: 99%