SUMMARY: Over the past decade, the history of tobacco’s introduction to Europe and its societal impact has been extensively studied, resulting in prevailing narratives about its adoption and consumption. In the Netherlands, historical records generally concur that: (I) tobacco rose in popularity among all socioeconomic classes between 1590 and 1630 CE; and (II) it spread throughout the Country as a male habit. However, the presence and consumption of tobacco have exhibited profound variations across diverse societies throughout history, manifesting dissimilar patterns of employment and significance over varying temporal and spatial dimensions. By analysing a sample of 351 human skeletons dating from 1300 to 1829 CE, the present study challenges the limited historical narratives presented above and emphasizes the diverse contextual factors that influenced tobacco’s prevalence in two different Dutch centres. Our results suggest that in certain areas of the Netherlands tobacco was likely present and widely consumed well before 1630 CE, while also highlighting overall substantial female participation in the practice. Furthermore, our analysis hints at the possibility of divergent methods of tobacco consumption between sexes, suggesting that the historical narrative of tobacco as solely a male habit may warrant reconsideration. Overall, our study contributes to a deeper understanding of the complex history of tobacco in the Netherlands, shedding light on historical trends and cultural practices.