Normal values for thrombelastography (ROTEM®) and selected coagulation parameters in porcine blood

Velik‐Salchner, Corinna; Schnürer, Christian; Fries, Dietmar; Müssigang, Peter R.; Moser, Patrizia; Streif, Werner; Kolbitsch, Christian; Lorenz, Ingo

doi:10.1016/j.thromres.2005.05.015

Cited by 123 publications

(96 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Plasma samples were stored at -80°C until analysis of t-PA activity (enzymelinked immunosorbent assay [ELISA], Technoclone TC16000), PAI-1 (Porcine PAI-1 Activity Assay; Dunn Labortechnik, Aspach, Germany) and TAT (Enzygnost TAT micro ELISA, Behring, Germany). Normal values for pigs have been published previously (13).…”

Section: Measurements Blood Sampling and Analytical Methodsmentioning

confidence: 99%

Thromboelastometry (TEM®) Findings in Disseminated Intravascular Coagulation in a Pig Model of Endotoxinemia

Schöchl

Solomon²,

Schulz

et al. 2010

Mol Med

View full text Add to dashboard Cite

Standard coagulation tests have a low specificity and sensitivity for diagnosing disseminated intravascular coagulation. The aim of this study was to determine whether whole blood thromboelastometry (TEM) detects lipopolysaccharide (LPS)-induced changes in coagulation. Blood samples from 10 pigs were drawn at baseline, before and at the end of LPS infusion and 2, 3, 4 and 5 h after the start of endotoxinemia. Simultaneous to TEM, standard coagulation tests and extended coagulation analysis including tissue plasminogen activator (t-PA) and plasminogen activator inhibitor 1 (PAI-1) were performed. Endotoxinemia resulted in a significant acceleration of the nonactivated TEM (NATEM) clotting time 2 h after the end of LPS infusion; in contrast, the changes in international normalized ratio and activated partial thromboplastin time suggested delayed initiation of coagulation. NATEM maximum clot firmness (MCF) and fibrin-based thromboelastometry test (FIBTEM ® )-MCF decreased significantly from baseline until the last time point (from 64.6 ± 7.8 and 35.1 ± 12.8 mm to 52.8 ± 4.6 and 21.4 ± 11.8 mm, respectively; P = 0.01 for both parameters). A sharp, transient increase of t-PA had no effect on maximum lysis in the NATEM test. PAI-1 increased significantly 3 h after the start of LPS infusion, paralleled by a decrease in maximum lysis. In conclusion, TEM was superior to standard coagulation tests in reflecting initial activation of coagulation during endotoxinemia. TEM further suggested consumption of coagulation substrate; at the same time, inhibition of plasminogen activation was accompanied by improved clot stability. Further investigations are necessary to establish the clinical relevance of these findings.

show abstract

Section: Measurements Blood Sampling and Analytical Methodsmentioning

confidence: 99%

Thromboelastometry (TEM®) Findings in Disseminated Intravascular Coagulation in a Pig Model of Endotoxinemia

Schöchl

Solomon²,

Schulz

et al. 2010

Mol Med

View full text Add to dashboard Cite

show abstract

“…However pigs have demonstrated significant variability in coagulation function response to trauma and hemorrhage (51,67,177,(200)(201)(202)(203). Both viscoelastic and plasma based assays of coagulation function have shown pigs to be relatively hypercoaguable compared to humans and to have protein C levels that are only 36% of human values (58,60). Given that activation of protein C is hypothesized to be a central driver of ATC, this fundamental difference may impede the ability to model ATC successfully in this species.…”

Section: Discussionmentioning

confidence: 99%

“…Animal models provide an alternative option as they facilitate the controlled and systematic evaluation of isolated insults in vivo, which may enhance mechanistic understanding and identify novel therapeutic targets. However animals are genetically distinct from humans, and demonstrate differences in coagulation assay parameters, coagulation factor concentrations and platelet function that may alter the susceptibility to ATC (58)(59)(60)(61)(62). For an animal model to be clinically relevant there needs to be more similarities than differences between the human coagulation system and that of the chosen animal species.…”

Section: Intrinsic Pathway Extrinsic Pathwaymentioning

confidence: 99%

“…Pigs are the only large animal species that has been used for evaluating ATC, which would appear appropriate given the established physiological similarities between pigs and humans (66). However comparative studies of coagulation function have shown pigs to be relatively hypercoagulable compared to humans, with protein C levels only 36% of human values (58,60). Given the hypothesized role of protein C in the development of ATC it is possible that this fundamental difference may impede the ability to successfully model ATC in pigs.…”

Section: Intrinsic Pathway Extrinsic Pathwaymentioning

confidence: 99%

“…However few have achieved proposed definitions of ATC, and evaluation of pathophysiological mechanisms was limited in most cases (63). Comparative studies of coagulation function have shown pigs to be relatively hypercoaguable compared to humans, which may impede the ability to model ATC in this species (58,60). The effect of non-traumatic hemorrhage of 30% blood volume on coagulation function has been evaluated in sheep (197); however there are no published ovine models evaluating the combined effects of trauma and hemorrhage .…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Validation of acute traumatic coagulopathy in an ovine model of trauma and haemorrhage

Zyl¹

View full text Add to dashboard Cite

This thesis describes an ovine model of complex trauma and haemorrhage that demonstrates coagulation changes using both traditional plasma based assays and point of care viscoelastic assays that are consistent with current definitions of ATC. The degree of coagulopathy was correlated with the degree of shock as quantified by arterial lactate.Coagulopathy was also associated with activation of the protein C pathway and shedding of the endothelial glycocalyx. Fibrinolysis did not make a significant contribution to the coagulopathy observed and there was no evidence of altered platelet function in this model.ii

show abstract

Hematology of Pigs

Thorn¹,

Bowman²,

Eckersall³

2022

Schalm's Veterinary Hematology

View full text Add to dashboard Cite

Normal values for thrombelastography (ROTEM®) and selected coagulation parameters in porcine blood

Cited by 123 publications

References 8 publications

Thromboelastometry (TEM®) Findings in Disseminated Intravascular Coagulation in a Pig Model of Endotoxinemia

Thromboelastometry (TEM®) Findings in Disseminated Intravascular Coagulation in a Pig Model of Endotoxinemia

Validation of acute traumatic coagulopathy in an ovine model of trauma and haemorrhage

Hematology of Pigs

Contact Info

Product

Resources

About