Normal spatial learning despite regional inhibition of LTP in mice lacking Thy-1

Nosten‐Bertrand, Marika; Er̀rington, M.L.; Murphy, Kerry P.S.J.; Tokugawa, Yoshihiro; Barboni, E.; Kozlova, Elena N.; Michalovich, David; Morris, Richard; Silver, Jack; Stewart, Colin L.; Bliss, T. V. P.; Morris, Roger J.

doi:10.1038/379826a0

Cited by 213 publications

(142 citation statements)

References 27 publications

Supporting

Mentioning

133

Contrasting

Unclassified

Order By: Relevance

“…Thy-1 À/À mice were a kind gift of Prof. R. Morris, King's College London [12]. Thy-1-deficient (Thy-1 À/À ) mice were established on a 129/Sv Â C57BL/6 background as described previously [12].…”

Section: Micementioning

confidence: 99%

“…Thy-1-deficient (Thy-1 À/À ) mice were established on a 129/Sv Â C57BL/6 background as described previously [12]. F2 littermates from the intercross of F1 Thy-1 1/À mice were used for comparative studies between Thy-1 À/À and Thy-1 1/1 mice.…”

Section: Micementioning

confidence: 99%

“…Thy-1-deficient mice, originally described by Nosten-Bertrand, are viable [12]. Due to the strong expression of Thy-1 on neuronal cells and T cells (TCs) in mice, previous studies in Thy-1-deficient mice were focused on the investigation of the nervous system and TC functions.…”

Section: Introductionmentioning

confidence: 99%

“…Mac-1 expressed on neutrophils and monocytes was identified as a counter receptor for Thy-1 [10]. Furthermore, Thy-1 provides not only the mechanical support for cell adhesion but also triggers neutrophil effector functions, such as the secretion of matrix metalloproteinases (MMP-9) and chemotactic factors (CXCL8) [10,11].Thy-1-deficient mice, originally described by Nosten-Bertrand, are viable [12]. Due to the strong expression of Thy-1 on neuronal cells and T cells (TCs) in mice, previous studies in Thy-1-deficient mice were focused on the investigation of the nervous system and TC functions.…”

mentioning

confidence: 99%

See 3 more Smart Citations

Thy‐1 (CD90) regulates the extravasation of leukocytes during inflammation

et al. 2011

View full text Add to dashboard Cite

Human Thy-1 (CD90) has been shown to mediate adhesion of inflammatory cells to activated microvascular endothelial cells via interaction with Mac-1 (CD11b/CD18) in vitro. Since there are no data showing the physiological relevance of Thy-1 for the recruitment of inflammatory cells in vivo, different inflammation models were investigated in Thy-1-deficient mice and littermate controls. In thioglycollate-induced peritonitis, the number of neutrophils and monocytes was significantly diminished in Thy-1-deficient mice. During acute lung inflammation, the extravasation of eosinophils and monocytes into the lung was significantly reduced in Thy-1-deficient mice. Moreover, during chronic lung inflammation, the influx of eosinophils and monocytes was also strongly decreased. These effects were independent of Thy-1 expression on T cells, as shown by the transplantation of WT BM into the Thy-1-deficient mice. In spite of the strong Thy-1 expression on T cells in the chimeric mice, the extravasation of the inflammatory cells in these mice was significantly diminished, compared to control mice. Finally, the altered number and composition of infiltrating leukocytes in Thy-1-deficient mice modified the chemokine/cytokine and protease expression at the site of inflammation. In conclusion, Thy-1 is involved in the control of inflammatory cell recruitment and, thus, also in conditioning the inflammatory microenvironment.Key words: Extravasation . Inflammation . Thy-1 Supporting Information available online IntroductionThe recruitment of inflammatory cells to sites of inflammation plays an important role in the pathogenesis of several inflammatory diseases. Leukocyte adhesion to endothelial cells (ECs) follows a multistep process, including the capture of free leukocytes out of the blood stream, rolling, firm adhesion, and transendothelial diapedesis. The importance of several adhesion molecules in this series of events has been described previously [1]. In ICAM-1-deficient mice, neutrophil recruitment was significantly reduced, but it was not completely blocked in a chemical peritonitis model or in a lipopolysaccharide 645(LPS)-induced airway inflammation model, indicating the involvement of additional adhesion molecules [2,3]. Furthermore, leukocyte recruitment in experimental colitis was not affected by blocking ICAM-1 or MadCAM, whereas the blocking of VCAM-1 resulted in a significant attenuation of colitis [4]. Thus, under specific inflammatory conditions, certain adhesion molecules mediate adhesion and transmigration of leukocytes into the perivascular tissue.Recently, human Thy-1 expressed on ECs was identified as an adhesion molecule mediating the binding of neutrophils and monocytes to activated microvascular ECs [5]. Thy-1 is a highly glycosylated GPI-anchored surface protein and a member of the immunoglobulin superfamily [6,7,8]. In humans, Thy-1 is expressed on ECs at sites of inflammation or in tumours whereas ECs do not express Thy-1 in healthy tissue [5,9]. Thy-1 is also expressed on fibroblasts, neurons, and a su...

show abstract

Section: Micementioning

confidence: 99%

Section: Micementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

mentioning

confidence: 99%

See 2 more Smart Citations

Thy‐1 (CD90) regulates the extravasation of leukocytes during inflammation

et al. 2011

View full text Add to dashboard Cite

show abstract

“…A variety of proteins from the immunoglobulin-like family, such as Thy-1, NCAM, L1, contactin, telencephalin and neuroplastin-65 are associated with alterations in hippocampal synaptic plasticity, including LTP and LTD (Muller et al, 1996;Nosten-Bertrand et al, 1996;Bliss et al, 2000;Nakamura et al, 2001;Murai et al, 2002). Empson et al (2006) showed that homophilic binding by neuroplastin-65 causes a sustained increase in phosphorylation of p38 MAPK, coincident with the p38 MAPK-sensitive loss of LTP and reduced surface expression of the GluR1 AMPAR subunit.…”

Section: Ampar Regulation By Cell Adhesion Molecules (Cams)mentioning

confidence: 99%

Regulation of AMPA receptors and synaptic plasticity

et al. 2009

View full text Add to dashboard Cite

Developmentally regulated expression of Thy-1 in structures of the mouse sensory-motor system

Barlow

Huntley

2000

J. Comp. Neurol.

View full text Add to dashboard Cite

Thy‐1 is a cell‐surface molecule of the immunoglobulin superfamily which is expressed at high levels in the mature nervous system. Thy‐1 has been implicated in regulating axonal outgrowth and synaptic function, but little is known regarding its cellular localization and expression in the central nervous system (CNS) during development or in adulthood. In this study, Thy‐1 gene expression and protein localization were examined in sensory‐motor and related areas of the adult and postnatally developing mouse CNS. Thy‐1 mRNA expression was restricted to neurons; immunoreactivity was densely distributed throughout the neuropil of all regions examined, often delineated the neuronal plasmalemma, and labeled axons in white matter tracts of the brain and spinal cord. In adulthood, immunolabeling was regionally widespread and was present relatively homogeneously throughout all cell‐dense layers of sensory‐motor cortex, throughout most thalamic nuclei, globus pallidus, and spinal cord. Developmentally, however, Thy‐1 expression and localization exhibited a spatially and temporally staggered sequence leading to the adult pattern. In sensory‐motor cortex, Thy‐1 expression in layer V preceded expression in other layers; in the barrel field, labeling of barrel septa preceeded a gradually increasing intensity of immunolabeling of barrel centers; in the thalamus, Thy‐1 exhibited a differential onset and temporal pattern of expression across different nuclei associated with motor, sensory, or limbic systems; in the caudate nucleus, Thy‐1 expression was greatest during the first postnatal week of life before declining during subsequent development. Taken together, the adult distribution and developmental patterns leading to it form a unique profile in comparison with other structurally related glycosyl‐phosphatidylinositol (GPI)‐anchored neural cell adhesion molecules. The pattern and timing of Thy‐1 expression across layers and nuclei during early postnatal development are more complex than previously recognized, thus perhaps reflecting varied roles for Thy‐1 in aspects of structural or functional maturation which proceed independently of the timing of neurogenesis, migration, and dendritic and axonal growth. J. Comp. Neurol. 421:215–233, 2000. © 2000 Wiley‐Liss, Inc.

show abstract

Normal spatial learning despite regional inhibition of LTP in mice lacking Thy-1

Cited by 213 publications

References 27 publications

Thy‐1 (CD90) regulates the extravasation of leukocytes during inflammation

Thy‐1 (CD90) regulates the extravasation of leukocytes during inflammation

Regulation of AMPA receptors and synaptic plasticity

Developmentally regulated expression of Thy-1 in structures of the mouse sensory-motor system

Contact Info

Product

Resources

About