Normal red blood cells partially decrease diepoxybutane‐induced chromosome breakage in cultured lymphocytes from Fanconi anaemia patients

Abstract

This study shows that DEB-induced chromosome instability in FA lymphocytes is partially reduced by normal RBCs. This effect may have some clinical relevance in vivo, whenever FA patients receive a RBC transfusion.

Cytogenetics evaluation of the effect of cord blood versus adult red blood cells on chromosomal breakage in Fanconi anemia

“…In agreement with the results of our study, Porto et al (2010) found that the presence of normal heterologous RBCs gave a statistically significant value in decreasing frequency of chromosomal breakage when compared with the presence of autologous RBCs. They found that chromosomal breakage of lymphocytes cultures with autologous RBCs was 9.8 breaks/aberrant cell, but with heterologous RBCs it was 6.2 breaks/aberrant cell.…”

“…Our patients' values are comparable with those previously reported in the literatures. Porto et al (2010) noticed that the mean of DEB-induced chromosomal breakage was 7.75 breaks/ cell; however, the results of our study are higher than those of Cirkovic et al (2011) who found that the range of breaks/cell was 0.26-4.39, with a mean breaks/cell 1.37.…”

“…They found that chromosomal breakage of lymphocytes cultures with autologous RBCs was 9.8 breaks/aberrant cell, but with heterologous RBCs it was 6.2 breaks/aberrant cell. Porto et al (2010) also noticed the reduction in DEBinduced chromosome breakage in lymphocyte cultures from FA patients performed 1 week after RBCs transfusion (4.5 breaks/aberrant cell) in comparison with lymphocyte cultures of the same patients performed before RBCs transfusion (8.9 breaks/aberrant cell). This finding suggested that normal heterologous RBCs have a valuable protective effect against DNA damage.…”

“…This may have important clinical implications in recovering the hematological status of FA patients and reducing chromosome instability (Porto et al, 2010).…”

“…Toxicity of the cross-linking diepoxybutane (DEB) is redox related and oxygen dependent . Consequently, cell protective systems against oxidative stress are involved in DEB detoxification, with red blood cells (RBCs) or some of its components in particular playing an important role (Porto et al, 2010).…”