Normal pulse polarography with carbon fiber electrodes for in vitro and in vivo determination of catecholamines

Ponchon, Jean Luc.; Cespuglio, Raymond; Gonon, François; Jouvet, Michel; Pujol, J.F.

doi:10.1021/ac50045a030

Cited by 332 publications

(126 citation statements)

References 38 publications

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“…Electrical connection is made between the fibre and a silver wire by inserting the latter into an epoxy resin/ graphite powder mixture previously pushed into the micropipette. The electrodes are left to solidify for 24-48h at room temperature (Ponchon et al, 1979;Wightman, 1981). Alternatively, electrical connection can be made by filling the micropipette with 0.9% NaCl solution into which is placed a silver wire or by using electrically conducting silver paint, providing the electrode tip is sealed afterwards (P. H. Hutson & G. Curzon, unpublished work).…”

Section: Recording Electrodes For Voltammetrymentioning

confidence: 99%

“…They therefore require more sensitive recording equipment and may be more susceptible to noise but because of the small currents produced they are less destructive to the species being oxidized. Ponchon et al (1979) used electrodes with 0.5mm of exposed fibre giving low residual currents of approx. 1 nA.…”

Section: Recording Electrodes For Voltammetrymentioning

confidence: 99%

“…Carbon fibre electrodes are reported to respond linearly in vitro to dopamine over the range 2 x 10-6-10-3M with a detection limit of 10-6M (Ponchon et al, 1979). After various pretreatments they responded linearly to dopamine (up to 2 x 10-6M), dihydroxyphenylacetic acid (up to 6 x 10-5M) and ascorbic acid (up to 10-3 M) (Gonon et al, 1980) and to 5-hydroxytryptophan and 5-hydroxytryptamine (10-7-10-6M) and 5-hydroxyindoleacetic acid (10-7-2 x 10-5M) (Cespuglio et al, 1981a).…”

Section: Modified Electrodesmentioning

confidence: 99%

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Monitoring in vivo of transmitter metabolism by electrochemical methods

Hutson¹,

Curzon²

1983

Biochemical Journal

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IntroductionConventional methods of studying the metabolism of central neurotransmitter amines (i.e. dopamine, noradrenaline and 5-hydroxytryptamine) in rats and other small laboratory animals have two major disadvantages. Firstly, animals under investigation must be killed before determinations can be made and secondly, intra-and extra-neuronal metabolism are not differentiated. Thus, determinations can only be made at one time per animal and information is not obtained on whether transmitters or metabolites measured reflect functional activity, i.e. transmitter molecules which had been available to receptors. Also, standard methods of measuring transmitter turnover cannot be applied to individual rats but require the use of groups of animals on which changes of amine, amine precursor or metabolite concentration are measured over a period after the administration of drugs which block transmitter synthesis or catabolism or egress of amine metabolites from the central nervous system.

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Section: Recording Electrodes For Voltammetrymentioning

confidence: 99%

Section: Recording Electrodes For Voltammetrymentioning

confidence: 99%

Section: Modified Electrodesmentioning

confidence: 99%

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Monitoring in vivo of transmitter metabolism by electrochemical methods

Hutson¹,

Curzon²

1983

Biochemical Journal

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“…L'apport de cet outil soulignait sans ambiguïté que le niveau de compréhension des mécanismes neurochimiques du sommeil était insuffisant. Pour dépasser les contradictions soulevées par les techniques successivement utilisées, la mise au point d'outils plus performants que ceux disponibles s'avérait de nouveau nécessaire [7]. Dans ce contexte expérimental, la réalisation d'un outil permettant la mesure de la libération de neurotransmetteur, la voltamétrie, a été entreprise au début des années 80 [8], [9].…”

Section: Cl-188 Annales De Physiqueunclassified

Fluorescence induite par laser sur les tissus profonds du cerveau du rat : application à l'étude du cycle veille-sommeil

Mottin

Laporte²,

Cespuglio³

et al. 1994

Ann. Phys. Fr.

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A nanosecond time resolved fluorescence, derived from the nucleus Raphe dorsalis (nRD) of the rat is reported. A single optical fiber is used for the transmission of the subnanosecond nitrogen laser pulse (337nm) and the collection of the NADH brain fluorescence at 460nm. This method also combined with polygraphic recordings, is used in animals under long-term chronic conditions and unanesthetized. Thoughout the Sleep-Wake cycle and versus the waking state (W), the NADH peak measured increases during slow wave sleep (SWS) and paradoxical sleep (PS) or during PS only. The variations observed are dependent upon the optical fiber location within the nRD. The existence of oxidation mechanisms for sleep triggering and maintenance are discussed.Resume: Un capteur chimique à fibre optique monofibre monobrin est utilisé pour transmettre le rayonnement d'excitation d'un laser azote et collecter la fluorescence liée au NADH cérébral. Cette méthode fluorimétrique couplée aux enregistrements polygraphiques est réalisée chez le rat en condition chronique long-terme. Au niveau du noyau du raphé dorsal (nRD) la fluorescence mesurée à 460nm présente une faible augmentation pour le sommeil paradoxal (SP) ainsi que dans certaines sous-structures pour le sommeil à onde lente (SL) par rapport au niveau enregistré pendant l'éveil; cette variation physiologique pourrait refléter l'activité dendritique. Ces mesures suggèrent une variation du métabolisme pendant le SP. La nature des mécanismes neurochimiques qui soustendent le SP est discutée. INTRODUCTIONLes capteurs chimiques à fibre optique (FOCS) ont commencé à apparaître à la fin des années soixante-dix sous la forme d'ajout de fibre optique à des appareils de mesure optique classique (fluorimètrie en cuvette) [1], [2]. A partir du milieu des années quatrevingt ils se sont diversifiés en plusieurs catégories: -les capteurs extrinsèques (la fibre optique restant passive); -semi-intrinsèques (la grandeur optique mesurée découle d'une reaction chimique localisée à la surface des fibres); -intrinsèques (les phénomènes de guide de lumière interviennent directement dans la mesure chimique) [3].Les secteurs d applications sont majoritairement le domaine de la chimie analytique, lié à t environnement, et le domaine biomédical.

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“…The use of electrodes with dimensions of micrometer range increased appreciably at the end of the seventies [4][5][6]. In contrast to electrodes of conventional dimensions, the measured current is considerably lower.…”

Section: Introductionmentioning

confidence: 99%

Electrochemical deposition of 5,10,15,20-tetrakis-(4-sulphonatophenyl) porphyrin and its Co(II) derivative at a gold microelectrode array

Wael

Adriaens

Temmerman

2005

Analytica Chimica Acta

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This paper describes the fabrication and surface modification of gold microelectrode array structures. The modification is done by continuous cycling of the electrode array in a pH 12 buffer solution containing 8.01 mmol L −1 5,10,15,20-tetrakis-(4-sulphonatophenyl)porphyrin tetrasodium salt (H 2 TSPor). Incorporation of gold ions in the ligand during the potential cycling of the gold electrode is assumed. Only ring processes appear in the current potential curves, which do not change with scan number, indicating a fast adsorption. A second modification was performed through the electrodeposition of 5,10,15,20-tetrakis-(4-sulphonatophenyl)porphyrin Co(II) (CoTSPor) at a gold micro-electrode array.

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Normal pulse polarography with carbon fiber electrodes for in vitro and in vivo determination of catecholamines

Abstract: An electrochemical microsystem using three electrodes is described. The active surface of the working electrode is a carbon fiber of 8-µ o.d. In normal pulse polarography, this

Cited by 332 publications

References 38 publications

Monitoring in vivo of transmitter metabolism by electrochemical methods

Monitoring in vivo of transmitter metabolism by electrochemical methods

Fluorescence induite par laser sur les tissus profonds du cerveau du rat : application à l'étude du cycle veille-sommeil

Electrochemical deposition of 5,10,15,20-tetrakis-(4-sulphonatophenyl) porphyrin and its Co(II) derivative at a gold microelectrode array

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