Normal percentage of CD56bright natural killer cells in young patients with a history of repeated unexplained implantation failure after in vitro fertilization cycles

Matteo, Maria; Greco, Pantaleo; Rosenberg, Piergiorgio; Mestice, Anna; Baldini, Domenico; Falagario, Teresa; Martino, V. Di; Santodirocco, Michele; Massenzio, Francesca; Castellana, Laura; Specchia, Giorgina; Liso, Arcangelo

doi:10.1016/j.fertnstert.2007.01.028

Cited by 47 publications

(29 citation statements)

References 24 publications

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“…Although, using fl ow cytometry, Matteo et al ( 2007 ) reported no difference in uNK cells in late secretory phase endometrium in women with RIF compared with normal fertile controls, others have reported increased uNK cells in pre-pregnancy endometrium in association with RIF. Tuckerman et al ( 2010 ) reported a dramatic increase in uNK cells as a proportion of stromal cells in the mid-secretory phase in women with RIF after IVF compared with normal fertile controls, with uNK cell numbers correlating with stromal expression of IL-15 (Mariee et al 2012 ).…”

Section: Recurrent Implantation Failure and Infertilitymentioning

confidence: 93%

The Role of Uterine NK Cells in Normal Reproduction and Reproductive Disorders

Bulmer

Lash

2015

Advances in Experimental Medicine and Biology

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The human endometrium contains a substantial population of leucocytes which vary in distribution during the menstrual cycle and pregnancy. An unusual population of natural killer (NK) cells, termed uterine NK (uNK) cells, are the most abundant of these cells in early pregnancy. The increase in number of uNK cells in the mid-secretory phase of the cycle with further increases in early pregnancy has focused attention on the role of uNK cells in early pregnancy. Despite many studies, the in vivo role of these cells is uncertain. This chapter reviews current information regarding the role of uNK cells in healthy human pregnancy and evidence indicating their importance in various reproductive and pregnancy problems. Studies in humans are limited by the availability of suitable tissues and the limitations of extrapolation from animal models.

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Section: Recurrent Implantation Failure and Infertilitymentioning

confidence: 93%

The Role of Uterine NK Cells in Normal Reproduction and Reproductive Disorders

Bulmer

Lash

2015

Advances in Experimental Medicine and Biology

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“…For example, while some previous case-control studies report a higher concentration of uNK cells in women with uRPL compared to fertile women 9,10,11,12,13 , other studies did not find this association 5,14 . Similarly, evidence exists for both an association and lack of association between uNK cell number/function and infertility 15,16,17,18 . Only few studies have examined changes of uNK cell numbers in endometriosis, demonstrating a lower percentage of CD56 + NK cells and a defect in NK activity in the eutopic endometrium of women with endometriosis 19, 20, 21, 22 .…”

Section: Introductionmentioning

confidence: 99%

Characterization of Uterine NK Cells in Women with Infertility or Recurrent Pregnancy Loss and Associated Endometriosis

Giuliani

Parkin

Lessey

et al. 2014

American J Rep Immunol

139

106

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Problem Uterine natural killer cells (uNK) have been thought to play a key role in endometriosis and infertility. We investigated the expression of CD56, CD16 and NKp46 in endometrial tissues from 61 women with unexplained recurrent pregnancy loss (uRPL) or infertility (UI), and correlated this with the presence or absence of endometriosis. The results from the patients with sub-fertility were compared to those from 10 fertile patients. Method of study Mid-secretory phase endometrial biopsies were obtained and the endometrial expression of CD56, CD16 or NKp46 was identified by immunohistochemistry and quantified (ImageJ Software). Results The percentage of CD16+ cells was higher in women with uRPL (7.9±3.2) and UI (9.0±5.5), even when these conditions were associated with endometriosis (8.9±5.3), compared to fertile patients (5.6±2.4, p<0.05). Likewise, the ratio of NKp46+:CD56+ cells was higher in women with uRPL (0.28±0.25) and UI (0.21±0.2), even when these conditions were associated with endometriosis (0.19±0.14), compared to fertile patients (0.1±0.1, p<0.05). No differences were observed when comparing CD56. Conclusions Women, with or without endometriosis, who have larger populations of cytotoxic CD16+ uNK cells and/or higher populations of NKp46+CD56+ cells may be at greater risk for infertility disorders resulting from an inflammtory environment occuring during implantation or later during decidualization.

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“…While Quenby et al propose that implantation failure relates to oxidative stress on endometrial tissue originating from endometrial edema formation, which is found with increased number of uNK cells and endometrial blood vessels [9]. On the other hand, Matteo et al found no difference in CD 56 + cells between the recurrent implantation failure group and the control group in a study performed with the flow cytometry technique which also includes CD 56 + cells from blood vessels [10]. Our study showed that the presence of CD 56 + cells in endometrial stromal tissue can be a destructive element for embryo implantation.…”

Section: Discussionmentioning

confidence: 99%

The Importance of CD56 and CD98 Levels in Patients with Recurrent Implantation Failure

Bildac�¹

2017

Ann Clin Anal Med

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ÖzetAmaç: Yardımcı üreme tekniklerinde gözlenen büyük gelişmelerin aksine klinik gebelik elde etme oranları taze embriyo transferlerinde %31, oosit donasyonlarında ise %41 düzeylerinde kalmıştır. İmplantasyon penceresi denilen dönem ve implantasyonun kendisi in-vitro fertilizasyon uygulamasında önemli bir yere sahiptir. Bu çalışma ile tekrarlayan implantasyon başarısızlığı olan in-vitro fertilizasyon hastalarında CD56 ve CD98 in immunohistokimyasal olarak boyanmasındaki farklar incelenmek istenmiştir. Gereç ve Yöntem: Bu çalışma 2004 ile 2010 yılları arasında in-vitro fertilizasyon yöntemi uygulanan 6260 hasta verisinin bulunduğu bir veribankasından tarama ile seçilen 36 hastanın örneklerinin değerlendirilmesi ile yapılmıştır. Tekrarlayan implantasyon başarısızlığı en az 3 kez in-vitro fertilizasyon denemesi yapılma-sı ve toplamda 8 embriyo verilmesini takiben b-HCG testinin pozitifleşmeme-si olarak tanımlanmıştır. Diğer yanda kontrol grubunda ise ilk in-vitro fertilizasyon denemelerinde b-HCG testinin pozitifleştiği hasta grubu bulunmaktadır. Bulgular: Yapılan karşılaştırmada CD 56 ve CD 98 boyanma yüzdeleri, boyanma gücü ve boyanma skoları arasında gruplar arasında anlamlı farklar saptanmıştır. (p<.001). Tekrarlayan implantasyon başarısızlığı olan hastaların endometriyal örneklerinde CD 98 boyanması kontrol grubuna gore anlamlı olarak daha azdır. Tartışma: CD 56 ve CD 98 in immunohistokimyasal olarak boyanmaları in-vitro fertilizasyon tedavisi uygulanacak olan hastalarda tanısal testlerin parçaları olabilirler. Bu markırların gebelik oluşumu ve gebelik sürecine olan genel etkilerinin araştırılması açısından daha ileri araştırma-lara gerek duyulmaktadır.Anahtar Kelimeler CD 56; CD 98; In-Vitro Fertilizasyon; İmplantasyon Başarısızlığı Abstract Aim: Despite major advances in assisted reproductive techniques, clinical pregnancy rates remain around 31% with fresh embryo transfer and around 41% with oocyte donations. We also know that the implantation process itself and the window period defined as the ''implantation phase'' are significantly important for successful in-vitro fertilization (IVF) cycles. With this study we have tried to determine any differences in immunohistochemical staining for CD56 and CD98 within the implantation phase endometrium of patients with recurrent implantation failure and of a control group that eventually had a successful IVF cycle. Material and Method: This study was retrospectively performed on a total of 36 patients selected out of a database of 6260 patients who received their IVF cycles from 2004 to 2010. Patients were defined as implantation failure if they did not have a positive result for b-HCG testing following at least 3 IVF cycles with a total of at least 8 embryo transfers. The control group was formed with patients who had success (positive b-HCG testing) on their first IVF treatment. Results: Comparison of means for CD 56 staining percentages, CD 98 staining percentages, CD 98 staining power, and CD 98 staining score showed significant difference between the control gr...

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Normal percentage of CD56bright natural killer cells in young patients with a history of repeated unexplained implantation failure after in vitro fertilization cycles

Cited by 47 publications

References 24 publications

The Role of Uterine NK Cells in Normal Reproduction and Reproductive Disorders

The Role of Uterine NK Cells in Normal Reproduction and Reproductive Disorders

Characterization of Uterine NK Cells in Women with Infertility or Recurrent Pregnancy Loss and Associated Endometriosis

The Importance of CD56 and CD98 Levels in Patients with Recurrent Implantation Failure

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