Normal macrophage function in infants receiving intralipid by low-dose intermittent administration

Strunk, Robert C.; Murrow, Bruce; Thilo, Elizabeth H.; Kunke, Kathleen; Johnson, Eugene G.

doi:10.1016/s0022-3476(85)80094-9

Cited by 23 publications

(10 citation statements)

References 24 publications

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“…Hydroxyl radical production in neonatal monocytes was also shown to be normal (27). \ , Generation of oxygen radicals in monocytes and monocytederived macrophages activated by lipopolysaccharide was identical in cells from newborns and adults (10,27). However, generation of chemiluminescence was similarly depressed in macrophages of either source, when compared with monocytes (20); this disparity between a normal generation of oxygen metabolites and a decreased chemiluminescence might be explained at least in part by a reduction in the content of excitable substrates The results of our study demonstrate that monocytes from newborns and adults similarly mature into macrophages during long-term culture.…”

Section: Resultsmentioning

confidence: 99%

“…The functions of neonatal macrophages have not been fully explored. Macrophages from newborns synthesize humoral host defense factors such as complement components C2, C4 to a similar extent as phagocytes from adults (9,10); fibronectin production was shown to be decreased in neonatal macrophages (9). Macrophages from newborns who had been activated by interferon-7 effectively killed or inhibited replication of toxoplasma gondii (1 I), presentation of bacterial Ag, and production of interleukin 1 was normal in cord blood monocytes/macrophages (12,13).…”

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confidence: 99%

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Phagocytosis-Associated Functions in Neonatal Monocyte-Derived Macrophages

et al. 1988

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A B S T R A n . Using a Teflon culture system we analyzed different aspects of cell maturation and phagocytic activities in neonatal monocyte-derived macrophages. Morphological and cytochemical characteristics as well as the protein composition of neonatal macrophages were identical with those of adult controls; actin binding protein (265,000 Da), myosin (210,000), a-actinin (102,000) and actin (42,000) could be identified in cells from either source: ~l l phagocytic functions were shown to be perfectly normal in neonatal macrophages when compared with adult cells: random migration, chemotactic response to zymosanactivated serum and formyl-methionyl-leucyl-phenylalanin, ingestion, and killing of Staphylococcus aureus, phagocytosis-associated chemiluminescence, production of oxygen intermediates (superoxide anion, 0;; hydrogenperoxide, H202). Phorbol myristate acetate-stimulated 0;-generation by 1 x lo5 macrophages was 11.8 f 4.7 nmol/h for neonates, and 10.2 f 3.9 for controls; production of H 2 0 2 was 7.6 f 3.5 nmol/h in neonatal macrophages and 6.4 +. In newborns, who suffer an increased susceptibility to serious systemic infections, subnormal phagocyte functions have been described. Neutrophils from neonates showed a decreased deformability (1) and a marked deficiency in movement toward different chemotactic stimuli (2-6). In addition generation of .OH by neonatal neutrophils was shown to be decreased (7). However, neonatal monocytes exhibited basically normal phagocytic functions when compared with adult cells (8). The functions of neonatal macrophages have not been fully explored. Macrophages from newborns synthesize humoral host defense factors such as complement components C2, C4 to a similar extent as phagocytes from adults (9, 10); fibronectin production Received October 12, 1987; accepted April 7, 1988. Correspondence Christian P. Speer, M.D., Universitats-Kinderklinik, RobertKoch-Str. 40, D-3400 Gottingen, Federal Republic of Germany.Supported by Grant Sp 239/2-2 from the Deutsche Forschungsgemeinschaft.2 1 was shown to be decreased in neonatal macrophages (9). Macrophages from newborns who had been activated by interferon-7 effectively killed or inhibited replication of toxoplasma gondii (1 I), presentation of bacterial Ag, and production of interleukin 1 was normal in cord blood monocytes/macrophages (12, 13). However, it has been suggested, that the decreased production of interferon-y by human neonatal cells (13) could be due to a functionally immature macrophage (14). Our study was initiated to examine the principal phagocyte activities of monocyte-derived macrophages in response to inflammation, i.e. chemotaxis, phagocytosis, killing, and generation of oxygen metabolites. Momholonical studies included a detailed analysis of the protein compos~ion of macrophages. MATERIALS AND METHODS Cell separation. Umbilical venous blood was withdrawn fromthe placenta into heparin (1-20 U/ml) immediately after delivery of healthy term newborns who were products of an uncomplicated pregnancy, normal labor, and...

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Section: Resultsmentioning

confidence: 99%

mentioning

confidence: 99%

Phagocytosis-Associated Functions in Neonatal Monocyte-Derived Macrophages

et al. 1988

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“…In infants, a low-dose intermittent LCT administration at the dose of 1 g/kg/d does not affect their macrophage function, serum levels of C2, C3, and C4, complement components synthesized and secreted exclusively in macrophages. 24 In 15 children who received PN for an average of 3 years, these clinically stable children had normal monocyte activation and normal complement C2, C3, and C4 levels. 25 The unchanged serum C3 and C4 levels in pediatric patients receiving 5-day LCT treatments found in the present study are consistent with these findings previously described in pediatric patients.…”

Section: Discussionmentioning

confidence: 92%

The Effects of a Short‐Term Long‐Chain‐Triglyceride Infusion on the Postoperative Immune Function of Pediatric Patients Receiving a Gastrointestinal Surgical Procedure

Ying

Zeng

et al. 2008

J Parenter Enteral Nutr

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“…To date, controlled clinical trials using recommended infusion rates have failed to demonstrate clinically significant alterations in (Table 3). [25][26][27][28][29][30][31] Studies assessing the influence of LCFA-based IVLE on cellular immunity have yielded variable results (Table 4). 26,[32][33][34][35] No clinical trials have shown significant alterations in components of humoral immunity, such as serum immunoglobulin or complement factor concentrations, as a result of LCFA-based IVLE (Table 5).…”

Section: Lipid Emulsions (Ivle)mentioning

confidence: 99%

“…26,[32][33][34][35] No clinical trials have shown significant alterations in components of humoral immunity, such as serum immunoglobulin or complement factor concentrations, as a result of LCFA-based IVLE (Table 5). 26,28,29 Investigators have been searching for alternative sources of lipids because of the risk of immunosuppression with LCFA-containing IVLE. Mediumchain fatty acids (MCFA) have been proposed as one such alternative because of differences in metabolism and clearance.…”

Section: Lipid Emulsions (Ivle)mentioning

confidence: 99%

Enhancing the Response to Parenteral Nutrition in Critical Care

Sacks

2004

Nut in Clin Prac

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Parenteral nutrition (PN) is an essential component in the support of critically ill patients with gastrointestinal dysfunction. Although PN cannot fully reverse hypermetabolism and accelerated skeletal muscle breakdown observed during periods of critical illness, it can prevent the adverse effects associated with malnutrition. The use of PN is not without complications, so care must be taken to ensure successful clinical outcomes with this complex therapy. Strategies have been developed by practitioners to promote safe practices with implementation of PN therapy and optimize a patient's response to PN. Identification of appropriate patient populations, strict glucose control, and manipulation of macro- and micronutrients are techniques being used to augment a patient's response to PN administration. This article will review the novel methods used to enhance benefits received from PN during critical illness.

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Normal macrophage function in infants receiving intralipid by low-dose intermittent administration

Cited by 23 publications

References 24 publications

Phagocytosis-Associated Functions in Neonatal Monocyte-Derived Macrophages

Phagocytosis-Associated Functions in Neonatal Monocyte-Derived Macrophages

The Effects of a Short‐Term Long‐Chain‐Triglyceride Infusion on the Postoperative Immune Function of Pediatric Patients Receiving a Gastrointestinal Surgical Procedure

Enhancing the Response to Parenteral Nutrition in Critical Care

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