Normal Human Fibroblasts Produce Membrane-Bound and Soluble Isoforms of FGFR-1

Root, Leslie; Shipley, Gary D.

doi:10.1006/mcbr.2000.0199

Cited by 21 publications

(29 citation statements)

References 42 publications

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“…CD56, or neural cell adhesion molecule, is a glycoprotein that binds fibroblast growth factor 1, which is constitutively expressed by fibroblasts. 46 The expression of CD56 on NK cells may be important in the differentiation of NK cells through contact with fibroblasts. 47 The functional purpose of CD56 expression of the monocyte/DC populations we observed here also remains unknown; however, it may also play a role in differentiation of these cell subsets.…”

Section: Discussionmentioning

confidence: 99%

Functionally distinct subsets of human NK cells and monocyte/DC-like cells identified by coexpression of CD56, CD7, and CD4

Milush

Long

Snyder‐Cappione

et al. 2009

Blood

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Section: Discussionmentioning

confidence: 99%

Functionally distinct subsets of human NK cells and monocyte/DC-like cells identified by coexpression of CD56, CD7, and CD4

Milush

Long

Snyder‐Cappione

et al. 2009

Blood

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“…25,26) The human derm is expresses multiple FGFs and predominantly FGF receptor 1, suggesting their important roles in maintaining normal dermal formation and function. 27,28) So far, although a total of 22 different FGF molecules have been described, aFGF and bFGF differ from most other FGFs in several important aspects: 27,28) aFGF and bFGF show strong homology (55%) in their amino acid sequences; mRNA expression of both aFGF and bFGF is detectable in a variety of tissues Diabetic patients with chronic skin ulcers were divided into two groups to receive topical application of either rhaFGF or rhbFGF at the same dose of 100 U/0.1 ml/cm 2 . After treatment, wound sites were photographed daily.…”

Section: Discussionmentioning

confidence: 99%

Comparison of the Therapeutic Effects Recombinant Human Acidic and Basic Fibroblast Growth Factors in Wound Healing in Diabetic Patients

Tan

Xiao²,

Huang³

et al. 2008

JOURNAL OF HEALTH SCIENCE

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To explore an optimal improvement of diabetic wound healing we have comparatively studied the effects of recombinant human acidic and basic fibroblast growth factors (rhaFGF and rhbFGF) on the healing impaired skin wounds in diabetic patients. Before using rhaFGF in diabetic patients, its pharmacokinetic features and possible toxic effects were evaluated using a rabbit model. For the 139 diabetic patients, 25% of them were divided into the group to receive topical rhbFGF daily at the dose of 100 U/0.1 ml/cm 2 as positive control (n = 35), and the remainder to receive topical rhaFGF at the same dose as rhbFGF (n = 104). Pharmacokinetic studies showed that plasma concentration of rhaFGF rapidly increased and reached to peak levels at 0.5 hr and then quickly decreased to normal levels at 3 hr after topical application on the wounds. No detectable toxic effects were found by examining multiple organs of the rabbits at 28 days after applying rhaFGF at 900 U/cm 2 . Topical application of either rhbFGF or rhaFGF at 100 U/cm 2 effectively cured the skin wounds in the diabetic patients. Although there was no remarkable difference between groups, rhaFGF provided a slightly better healing rate and efficiency than rhbFGF did. Therefore rhaFGF will become an alternative candidate for diabetic wounds, especially when diabetic wounds were in acidic environment due to bacterial infection.

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“…The former is involved in homophilic adhesion, whereas the latter can bind to the third Ig domain of FGFR1 through its second fibronectin type III domain (17). FGFR1 is constitutively expressed on fibroblasts in both membrane-bound and secreted forms (9), and ligation by CD56 can induce a signal transduction cascade in cells expressing FGFR1 (18 bright cells differentiated to CD56 dim upon in vitro culture with synovial or dermal fibroblasts for a comparable time, and this only increased to a maximum of 40% after longer culture (data not shown). These data suggest that other cell types or factors may be important in this process in vivo.…”

Section: Cd56 Bright Cells Have Longer Telomeres Than Cd56 Dim Nk Cellsmentioning

confidence: 99%

“…Third, as originally proposed by Nagler et al (8), CD56 bright may be precursors of CD56 dim (or vice versa). CD56 is a ligand for fibroblast growth factor receptor (FGFR)1 that is constitutively expressed on fibroblasts in both membrane-bound and secreted forms (9). We hypothesized that heterophilic adhesion of CD56 bright NK cells with peripheral tissue fibroblasts via a CD56-FGFR1 interaction may aid the differentiation into CD56 dim cells.…”

mentioning

confidence: 99%

CD56bright Human NK Cells Differentiate into CD56dim Cells: Role of Contact with Peripheral Fibroblasts

Chan

Hong

Atzberger

et al. 2007

The Journal of Immunology

297

235

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Human NK cells are divided into CD56brightCD16− cells and CD56dimCD16+ cells. We tested the hypothesis that CD56bright NK cells can differentiate into CD56dim cells by prospectively isolating and culturing each NK subset in vitro and in vivo. Our results show that CD56bright cells can differentiate into CD56dim both in vitro, in the presence of synovial fibroblasts, and in vivo, upon transfer into NOD-SCID mice. In vitro, this differentiation was inhibited by fibroblast growth factor receptor-1 Ab, demonstrating a role of the CD56 and fibroblast growth factor receptor-1 interaction in this process. Differentiated CD56dim cells had reduced IFN-γ production but increased perforin expression and cytolysis of cell line K562 targets. Flow cytometric fluorescent in situ hybridization demonstrated that CD56bright NK cells had longer telomere length compared with CD56dim NK cells, implying the former are less mature. Our data support a linear differentiation model of human NK development in which immature CD56bright NK cells can differentiate into CD56dim cells.

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Normal Human Fibroblasts Produce Membrane-Bound and Soluble Isoforms of FGFR-1

Cited by 21 publications

References 42 publications

Functionally distinct subsets of human NK cells and monocyte/DC-like cells identified by coexpression of CD56, CD7, and CD4

Functionally distinct subsets of human NK cells and monocyte/DC-like cells identified by coexpression of CD56, CD7, and CD4

Comparison of the Therapeutic Effects Recombinant Human Acidic and Basic Fibroblast Growth Factors in Wound Healing in Diabetic Patients

CD56bright Human NK Cells Differentiate into CD56dim Cells: Role of Contact with Peripheral Fibroblasts

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