The study aims to understand the interferon-gamma (IFN-γ) level and affecting factors in diabetes mellitus (DM) patients at Public Health Center (PHC). This cross-sectional study was conducted on 153 DM patients. The inclusion criteria were male or female patients over 18 years old diagnosed with DM for three months. They stated their willingness to study Interviews were conducted to obtain exercise habits data. We carried out laboratory measurements of random blood sugar(RBS), triglycerides TG, and IFN-γ. Data are presented descriptively and analytically. Most subjects were women with no smoking, hyperglycemia, and hypertriglyceridemia. RBS and TG were higher than the target, 257.88 ± 117.76 mg/dl and 204.66 ± 103.83 mg/dl, respectively. Hemoglobin (Hb) (12.95 ± 1.49), leukocyte (8.96 ± 1.96), platelet (321.24 ± 0.74), and erythrocytes (4.53 ± 0.55) were normal. There was no difference in IFN-γ levels based on gender, age group, obesity status, hyperglycemia status, hypertriglyceridemia, and hypertension status. Conclusion: There was no difference in IFN-γ levels based on demographic and clinical characteristics in DM patients at the JPHC.