In this work, a Josephson relation is generalized to a multi-component fermion superfluid. Superfluid density is expressed through a two-particle Green function for pairing states. When the system has only one gapless collective excitation mode, the Josephson relation is simplified, which is given in terms of the order parameters and the trace of two-particle normal Green function. In addition, it is found that the two-particle Green function is directly related to the pairing fluctuations of order parameters. Further more, in the presence of inversion symmetry, the superfluid density is given in terms of the pairing fluctuation matrix. The results of the superfluid density in Haldane model show that the generalized Josephson relation can be also applied to a multi-band fermion superfluid in lattice.