Normal and experimentally induced lysosomal activity in the larval fat body of Calliphora erythrocephala Meigen

Pelt-Verkuil, E. van; Rongen, Eric van; Priester, W. de

doi:10.1007/bf00233273

Cited by 15 publications

(2 citation statements)

References 12 publications

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“…This situation may be caused by factors missing from the hemolymph. Hydrolytic enzymes which are important for initiation of destruction are mostly made by fat bodies and are released into the hemolymph (Van Pelt-Verkuil, 1979a, 1979b. Initially degenerated basal lamina is then attacked and the tissue phagoeytosed by hemocytes, as was observed in vivo by yon Gaudecker (1972) and us (not shown).…”

Section: Discussionmentioning

confidence: 68%

Ultrastructural changes of Drosophila larval and prepupal salivary glands cultured in vitro with ecdysone

Farkas

Sutáková

1998

In Vitro Cell.Dev.Biol.-Animal

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Alterations in the ultrastructure of in vitro cultured larval salivary glands of Drosophila melanogaster in response to the steroid hormone ecdysone were studied in relation to complex changes in puffing patterns. We found that the changes in the fine structure of cultured glands reflected progression of the puffing pattern, and they paralleled those seen in vivo. We observed that glue secretion by exocytosis, the main function of salivary glands, took place between puff stage 5 (PS5) and PS7. Glue could not be expectorated under culture conditions but was slowly released from the lumen through a duct into the medium. After the cultured glands reached PS13/PS14, further progress of puffing and fine structural alterations required that the ecdysteroid titer be transiently extremely low or absent. Under in vitro conditions we did not observe the putative new secretory program(s) described for glands in vivo after PS12. However, ultrastructural changes which unambiguously indicated that an autohistolytic process had begun in vitro started to appear after PS17. Many salivary gland cells developed numerous features of progressive self-degradation between PS18 and PS21. Actual degradation of salivary glands in vivo seemed to be rapid, but in vitro degradation was never completed, probably due to a lack of exogenous factors from the hemolymph. Manipulations of ecdysone titer in vitro in the culture medium, known during the larval puffing cycle to cause premature induction of developmentally specific puffing patterns, did not affect the normal development of ultrastructural features of the cytoplasm and nucleus.

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Section: Discussionmentioning

confidence: 68%

Ultrastructural changes of Drosophila larval and prepupal salivary glands cultured in vitro with ecdysone

Farkas

Sutáková

1998

In Vitro Cell.Dev.Biol.-Animal

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“…It is decomposed during larval-pupal metamorphosis through a process involving formation of autophagic vacuoles, increased synthesis of lysosomal enzymes and accumulation of protein storage granules [72][73][74]. In Mamestra brassicae, this autophagic process is induced by 20E [75,76] and it seems to be regulated by cAMP.…”

Section: B Autophagy In Insect Development and Metamorphosismentioning

confidence: 99%

Autophagy in Invertebrates: Insights Into Development, Regeneration and Body Remodeling

Tettamanti

Saló²,

Gonzalez-Estevez³

et al. 2008

CPD

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Autophagy is a process in which eukaryotic cells sequester and degrade cytoplasm and organelles via the lysosomal pathway. This process allows turnover of intracellular organelles, participates in the maintenance of cellular homeostasis and prevents accumulation of defective cellular structures. Increased autophagy is normally induced by environmental cues such as starvation and hormones, while excessive levels of autophagy can lead to autophagic programmed cell death (PCD), with features that differ from those of the apoptotic PCD process. Since autophagic PCD plays a key role in development, morphogenesis and regeneration in several animal taxa, identification of evolutionarily conserved components of the autophagic machinery is a basic starting point in order to unravel the role of autophagy under both physiological and pathological conditions. Here we summarize recent findings on the role of autophagy in two different invertebrate taxa, Platyhelminthes and Insects, focusing attention on two complex events occurring in those systems, namely planarian regeneration and insect metamorphosis. Both represent good models in which to investigate the process of autophagy and its relationship with other PCD mechanisms.

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Autophagocytosis in the larval midgut cells of Pieris brassicae during metamorphosis

Kömüves¹,

Sass²,

Kovács³

1985

Cell Tissue Res.

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Normal and experimentally induced lysosomal activity in the larval fat body of Calliphora erythrocephala Meigen

Cited by 15 publications

References 12 publications

Ultrastructural changes of Drosophila larval and prepupal salivary glands cultured in vitro with ecdysone

Ultrastructural changes of Drosophila larval and prepupal salivary glands cultured in vitro with ecdysone

Autophagy in Invertebrates: Insights Into Development, Regeneration and Body Remodeling

Autophagocytosis in the larval midgut cells of Pieris brassicae during metamorphosis

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