Normal and abnormal development of the cardiac conduction system; implications for conduction and rhythm disorders in the child and adult

Jongbloed, Monique R.M.; Steijn, Rebecca Vicente; Hahurij, Nathan D.; Kelder, Tim P.; Schalij, Martin J.; Groot, Adriana C. Gittenberger‐de; Blom, Nico A.

doi:10.1016/j.diff.2012.04.006

Cited by 43 publications

(25 citation statements)

References 195 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…al. in this issue) [153]. Though the zebrafish heart is comprised of only two chambers (one atrium and one ventricle), recent physiologic studies in the zebrafish using optical mapping (calcium and voltage sensitive dyes or transgenics), electrophysiologic analysis, and optogenetics have discovered that a cardiac electrical wiring system, similar to four-chambered vertebrate hearts, is required to coordinate the contractions of the zebrafish atrial and ventricular chambers [11, 17, 154] (Figure 4I, J and 5A, B).…”

Section: Functional Heart Defectsmentioning

confidence: 99%

Zebrafish models in cardiac development and congenital heart birth defects

Neil

2012

Differentiation

View full text Add to dashboard Cite

The zebrafish has become an ideal vertebrate animal system for investigating cardiac development due to its genetic tractability, external fertilization, early optical clarity and ability to survive without a functional cardiovascular system during development. In particular, recent advances in imaging techniques and the creation of zebrafish transgenics now permit the in vivo analysis of the dynamic cellular events that transpire during cardiac morphogenesis. As a result, the combination of these salient features provides detailed insight as to how specific genes may influence cardiac development at the cellular level. In this review, we will highlight how the zebrafish has been utilized to elucidate not only the underlying mechanisms of cardiac development and human congenital heart diseases (CHDs), but also potential pathways that may modulate cardiac regeneration. Thus, we have organized this review based on the major categories of CHDs – structural heart, functional heart, and vascular/great vessel defects, and will conclude with how the zebrafish may be further used to contribute to our understanding of specific human CHDs in the future.

show abstract

Section: Functional Heart Defectsmentioning

confidence: 99%

Zebrafish models in cardiac development and congenital heart birth defects

Neil

2012

Differentiation

View full text Add to dashboard Cite

show abstract

“…Disturbances in the normal electrical activation pattern of the heart resulting in cardiac arrhythmias are an important cause of mortality and morbidity in the general population 1.…”

Section: Introductionmentioning

confidence: 99%

The sinus venosus myocardium contributes to the atrioventricular canal: potential role during atrioventricular node development?

Kelder

Vicente‐Steijn

Harryvan

et al. 2015

J Cellular Molecular Medi

Self Cite

View full text Add to dashboard Cite

The presence of distinct electrophysiological pathways within the atrioventricular node (AVN) is a prerequisite for atrioventricular nodal reentrant tachycardia to occur. In this study, the different cell contributions that may account for the anatomical and functional heterogeneity of the AVN were investigated. To study the temporal development of the AVN, the expression pattern of ISL1, expressed in cardiac progenitor cells, was studied in sequential stages performing co-staining with myocardial markers (TNNI2 and NKX2-5) and HCN4 (cardiac conduction system marker). An ISL1+/TNNI2+/HCN4+ continuity between the myocardium of the sinus venosus and atrioventricular canal was identified in the region of the putative AVN, which showed a pacemaker-like phenotype based on single cell patch-clamp experiments. Furthermore, qPCR analysis showed that even during early development, different cell populations can be identified in the region of the putative AVN. Fate mapping was performed by in ovo vital dye microinjection. Embryos were harvested and analysed 24 and 48 hrs post-injection. These experiments showed incorporation of sinus venosus myocardium in the posterior region of the atrioventricular canal. The myocardium of the sinus venosus contributes to the atrioventricular canal. It is postulated that the myocardium of the sinus venosus contributes to nodal extensions or transitional cells of the AVN since these cells are located in the posterior region of the AVN. This finding may help to understand the origin of atrioventricular nodal reentrant tachycardia.

show abstract

“…The reduction of the SAN volume can be caused by either diminished growth or increased decomposition of the SAN cells. Mutant mouse models of genes expressed in the posterior second heart field (including the Shox2, Podoplanin, Id2, Tbx3 and Pdgfr-alpha mutant mouse embryos [5]), largely share a phenotype with hypoplasia of the cardinal (putative caval) vein myocardium, hypoplasia of the SAN, aberrant expression patterns of Connexins and NKX2.5 and sinus node bradycardia. VEGF is expressed in the sinus venosus myocardium, however in Vegf 120/120 mutants no overt hypoplasia of sinus venosus myocardium was observed.…”

Section: Bradycardia Inmentioning

confidence: 99%