Nordihydroguaiaretic acid is a potentin vitroscavenger of peroxynitrite, singlet oxygen, hydroxyl radical, superoxide anion and hypochlorous acid and preventsin vivoozone-induced tyrosine nitration in lungs

Abstract

The antioxidant nordihydroguaiaretic acid (NDGA) has recently become well known as a putative anticancer drug. In this paper, it was evaluated the in vitro peroxynitrite (ONOO(-)), singlet oxygen ((1)O(2)), hydroxyl radical (OH(v)), hydrogen peroxide (H(2)O(2)), superoxide anion and hypochlorous acid (HOCl) scavenging capacity of NDGA. It was found that NDGA scavenges: (a) ONOO(-) (IC(50) = 4 +/- 0.94 microM) as efficiently as uric acid; (b) (1)O(2) (IC(50) = 151 +/- 20 microM) more efficiently than dimethyl thiourea, lipoic acid, N-acetyl-cysteine and glutathione; (c) OH(v) (IC(50) = 0.15 +/- 0.02 microM) more efficiently than dimethyl thiourea, uric acid, trolox, dimethyl sulfoxide and mannitol, (d) (IC(50) = 15 +/- 1 microM) more efficiently than N-acetyl-cysteine, glutathione, tempol and deferoxamine and (e) HOCl (IC(50) = 622 +/- 42 microM) as efficiently as lipoic acid and N-acetyl-cysteine. NDGA was unable to scavenge H(2)O(2). In an in vivo study in rats, NDGA was able to prevent ozone-induced tyrosine nitration in lungs. It is concluded that NDGA is a potent in vitro scavenger of ONOO(-), (1)O(2), OH(v), and HOCl and is able to prevent lung tyrosine nitration in vivo.

Antioxidant Therapy as a Potential Approach to Severe Influenza-Associated Complications

“…The ability of NDGA to scavenge the above-mentioned oxygen radicals is much greater than that of reference tested compounds, such as uric acid, penicillamine, reduced glutathione and mannitol [56]. These strong antioxidant properties may be due to the presence of four reducing equivalents from the two catechol groups in NDGA; hydrogen atoms of the four phenolic hydroxyl groups react with oxygen radicals [56]. NDGA has been shown to have promising applications in the treatment of multiple diseases, including cardiovascular diseases, neurological disorders, cancers and virus infections [57].…”

“…NDGA scavenges oxygen radicals, such as peroxynitrite, singlet oxygen, hydroxyl and superoxide anion radicals [56]. The ability of NDGA to scavenge the above-mentioned oxygen radicals is much greater than that of reference tested compounds, such as uric acid, penicillamine, reduced glutathione and mannitol [56].…”

“…NDGA scavenges oxygen radicals, such as peroxynitrite, singlet oxygen, hydroxyl and superoxide anion radicals [56]. The ability of NDGA to scavenge the above-mentioned oxygen radicals is much greater than that of reference tested compounds, such as uric acid, penicillamine, reduced glutathione and mannitol [56]. These strong antioxidant properties may be due to the presence of four reducing equivalents from the two catechol groups in NDGA; hydrogen atoms of the four phenolic hydroxyl groups react with oxygen radicals [56].…”

Luminescence Amplification by Enzymatic Eu2+ Oxidation to Eu3+ for Time-Resolved Peroxidase Activity Measurement

“…25 The results of NDGA addition are shown in Fig. 6, and as expected, NDGA decreased dose-dependently the Eu 3+ luminescence intensity at sham, 4 h, and 8 h.…”

In Vitro Studies on Antioxidant and Antidiabetic Activity of Pleurotus Eous Mushroom in Methanol and Aqueous Extract

“…This activity was determined according to the standard method with minor changes [24], take different concentrations of samples and standard, and add equal volume of H 2 O 2 in test tubes. To this, add 10 µL of methanol and 900 µL of FOX reagent.…”