Norcantharidin inhibits viability and induces cell cycle arrest and apoptosis in osteosarcoma

Zhu, Yingchun; Mi, Yunfeng; Wang, Zheyang; Jia, Xuewen; Jin, Zhanping

doi:10.3892/ol.2018.9615

Cited by 9 publications

(7 citation statements)

References 26 publications

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“…NCTD can inhibit the proliferation of osteosarcoma cells by blocking the Akt/mTOR signal transduction pathway and inducing G2/M cell cycle arrest in 143B and SJSA osteosarcoma cells in a dose-and time-dependent manner. At the same time, NCTD led to the upregulation of cleaved caspase-3 and an increase in the downstream target cleaved PARP, which suggested that the extrinsic pathway may also be involved in NCTD-induced cell apoptosis [22]. By evaluating the effects of NCTD on OS cell lines (MG63 and HOS) in vitro and in vivo, we found that NCTD can inhibit cell cycle and induce apoptosis of human OS cells.…”

Section: Introductionmentioning

confidence: 82%

[Retracted] Strontium/Chitosan/Hydroxyapatite/Norcantharidin Composite That Inhibits Osteosarcoma and Promotes Osteogenesis In Vitro

Huang

Sun

Lü

et al. 2020

BioMed Research International

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Hydroxyapatite can deliver drugs, and its composite material is capable of repairing bone defects in tumors. This study was conducted to evaluate the effect of composite materials on tumor growth inhibition and bone growth induction. Composites containing drug delivery compounds were synthesized by coprecipitation and freeze-drying and then characterized by scanning electron microscopy (SEM), X-ray diffraction (XRD), and Fourier-transform infrared spectroscopy (FTIR). In addition, the effect of hydroxyapatite nanoparticles (nano-SHAP) on proliferation of an osteosarcoma cell line (MG-63) and an osteoblast cell line (MC3T3-E1) was evaluated, and its mechanism was studied. The use of nano-SHAP alone did not affect the proliferation of normal cell lines. However, nanoparticles containing different amounts of norcantharidin in the composite materials and had different inhibitory effects on osteosarcoma and different effects on osteoblasts. And, with the increase of the content of norcantharidin, the antitumor performance of the composite has been enhanced. In summary, the nano-SHAP system developed in this study is a drug delivery material that can inhibit the growth of tumors and induce the proliferation of osteoblasts.

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Section: Introductionmentioning

confidence: 82%

[Retracted] Strontium/Chitosan/Hydroxyapatite/Norcantharidin Composite That Inhibits Osteosarcoma and Promotes Osteogenesis In Vitro

Huang

Sun

Lü

et al. 2020

BioMed Research International

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“…In addition, we identified seven TCM drugs that target CYP2E1. Recent research has provided evidence that natural active ingredients in TCM drugs have practical antitumor therapeutic effects on solid tumors 42 . Network pharmacology has been extensively applied by researchers 43 .…”

Section: Discussionmentioning

confidence: 99%

“…Recent research has provided evidence that natural active ingredients in TCM drugs have practical antitumor therapeutic effects on solid tumors. 42 Network pharmacology has been extensively applied by researchers. 43 18beta‐glycyrrhetinic acid, styrene, toluene, nicotine, m‐xylene, p‐xylene, and colchicine may play a role in gliomas by influencing CYP2E1, which requires further study.…”

Section: Discussionmentioning

confidence: 99%

Downregulation of CYP2E1 is associated with poor prognosis and tumor progression of gliomas

Wang

et al. 2021

Cancer Medicine

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Objective To explore the role and possible regulatory mechanisms of CYP2E1 in gliomas. Methods RNA‑seq data and corresponding clinical information of glioma patients were collected from The Cancer Genome Atlas and Chinese Glioma Genome Atlas, and mRNA data of normal brain tissues were obtained by the Genotype‐Tissue Expression project. The Wilcoxon test was performed to analyze the correlation between CYP2E1 expression and glioma subtypes. Univariate and multivariate Cox proportional hazards regression, receiver operating characteristic curves, and Kaplan–Meier plots were used to evaluate the prognostic value of CYP2E1 in glioma. Functional enrichment analyses and immune infiltration analyses were performed to investigate the potential function of CYP2E1 in gliomas. Moreover, we investigated the miRNA and epigenetic mechanisms that regulate CYP2E1 expression. Finally, network pharmacology and molecular docking experiments were used to predict drugs that target CYP2E1. Results The downregulation of CYP2E1 expression may predict a poor prognosis for glioma patients. CYP2E1 expression decreased with increasing WHO grade (II–IV), and its level was correlated with clinical features, including age, 1p19q codeletion status, and IDH state in glioma tissues. Furthermore, CYP2E1 was involved in lipid metabolism and ferroptosis and related to the tumor immune microenvironment due to its strong correlation with the levels of infiltrating monocytes and Tregs. Moreover, variation in the total methylation level and copy number of CYP2E1 was moderately correlated with its mRNA expression (p < 0.05). CYP2E1 was predicted to be targeted by hsa‐miR‐527, whose expression was negatively related to CYP2E1 mRNA expression (p < 0.05). In addition, effective compounds that target CYP2E1, including 18beta‐glycyrrhetinic acid, styrene, toluene, nicotine, m‐xylene, p‐xylene, and colchicine, were identified. Conclusion The downregulation of CYP2E1, which affects lipid metabolism and the ferroptosis signaling pathway, promotes the progression of gliomas.

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“…Previous studies indicated that NCTD 24 and PTX 25 induced cell cycle arrest in multiple cancer cells. Moreover, this study next clarified whether NCTD combined with PTX induces cell cycle arrest.…”

Section: Resultsmentioning

confidence: 97%

Norcantharidin combined with paclitaxel induces endoplasmic reticulum stress mediated apoptotic effect in prostate cancer cells by targeting SIRT7 expression

Hui

Chen

et al. 2021

Environmental Toxicology

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Prostate cancer (PCa), an extremely common malignancy in males, is the most prevalent disease in several countries. Norcantharidin (NCTD) has antiproliferation, antimetastasis, apoptosis, and autophagy effects in various tumor cells. Nevertheless, the antitumor effect of NCTD combined with paclitaxel (PTX), a chemotherapeutic drug, in PCa remains unknown. The cell growth, proliferative rate, cell cycle distribution, and cell death were determined by 3‐[4,5‐dimethylthiazol‐2‐yl]‐2,5 diphenyltetrazolium bromide, colony formation assay, PI staining, and Annexin V/PI staining by flow cytomertry, whereas the mitochondrial membrane potential (MMP) and endoplasmic reticulum (ER) stress was evaluated using the MitoPotential assay and ER‐ID red assay. We also evaluated the protein and mRNA expression of SIRTs by Western blotting and qRTPCR assay. Overexpression effectivity was measured by DNA transfection assay. Our study showed that cell viability and proliferative PC3 and DU145 rates were effectively inhibited after NCTD‐PTX combination. We also found that NCTD‐PTX combination treatment significantly enhance G2/M phase arrest, induction of cell death and ER stress, loss of MMP, and ER‐ or apoptotic‐related protein expression. Furthermore, NCTD‐PTX combination treatment was significantly decreasing the protein and mRNA expression of SIRT7 in PCa cells. Combination therapy effectively reduced cell viability, ER stress‐mediated apoptosis and p‐eIF2α/ATF4/CHOP/cleaved‐PARP expression inhibition in SIRT7 overexpression of PCa cells. These results indicate that NCTD combined with PTX induces ER stress‐mediated apoptosis of PCa cells by regulating the SIRT7 expression axis. Moreover, combination therapy may become a potential therapeutic strategy against human PCa.

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Norcantharidin inhibits viability and induces cell cycle arrest and apoptosis in osteosarcoma

Cited by 9 publications

References 26 publications

[Retracted] Strontium/Chitosan/Hydroxyapatite/Norcantharidin Composite That Inhibits Osteosarcoma and Promotes Osteogenesis In Vitro

[Retracted] Strontium/Chitosan/Hydroxyapatite/Norcantharidin Composite That Inhibits Osteosarcoma and Promotes Osteogenesis In Vitro

Downregulation of CYP2E1 is associated with poor prognosis and tumor progression of gliomas

Norcantharidin combined with paclitaxel induces endoplasmic reticulum stress mediated apoptotic effect in prostate cancer cells by targeting SIRT7 expression

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