1996
DOI: 10.1016/0006-2952(96)00344-9
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Noradrenaline stimulation of high-affinity GTPase activity in membranes from rat aorta and caudal artery

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Cited by 6 publications
(2 citation statements)
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“…A requirement for GTP to maintain Ca 2+ sensitivity in the absence of noradrenaline has also been noted previously (Akata, 1996). It was of interest that the concentration of GTP required (2 μM) was similar to the K m value reported for the high affinity GTPase activity of GTP‐binding proteins in caudal artery membranes (in the range from 0.24 to 1 μM, Weber & MacLeod, 1996). The concentration of GTP required was, however, two orders of magnitude less than the concentrations of free GTP which have been reported in smooth muscle (Coburn, Azim, Fillers & Baron, 1993), and it is therefore unlikely, as was pointed out by these authors, that changing levels of GTP in vivo could be a limiting or regulatory factor for contraction.…”
Section: Discussionsupporting
confidence: 67%
“…A requirement for GTP to maintain Ca 2+ sensitivity in the absence of noradrenaline has also been noted previously (Akata, 1996). It was of interest that the concentration of GTP required (2 μM) was similar to the K m value reported for the high affinity GTPase activity of GTP‐binding proteins in caudal artery membranes (in the range from 0.24 to 1 μM, Weber & MacLeod, 1996). The concentration of GTP required was, however, two orders of magnitude less than the concentrations of free GTP which have been reported in smooth muscle (Coburn, Azim, Fillers & Baron, 1993), and it is therefore unlikely, as was pointed out by these authors, that changing levels of GTP in vivo could be a limiting or regulatory factor for contraction.…”
Section: Discussionsupporting
confidence: 67%
“…It has previously been showed that aadrenoceptors and especially a 1 -adrenoceptors, can couple to multiple transduction pathways (Orowio et al, 1992;GarciaSainz, 1993) including G i -protein-mediated pathways (Fujiwara et al, 1989;Aburto et al, 1993;D'Angelo & Osol, 1994). PTX-sensitive G i -proteins have been isolated from the rat tail artery smooth muscle cell (Weber & MacLeod, 1996;Petitcolin et al, 1998). The present results show that in the de-endothelialized rat tail artery, PTX ADP-ribosylates G iproteins.…”
Section: Discussionmentioning
confidence: 99%