Noradrenaline-induced spontaneous inhibitory postsynaptic currents in mouse basolateral nucleus of amygdala pyramidal neurons: Comparison with dopamine-induced currents

Miyajima, Moeko; Yamada, Daisuke; Ozaki, Makoto; Wada, Keiji; Sekiguchi, Masayuki

doi:10.1016/j.neures.2011.07.936

Cited by 4 publications

(6 citation statements)

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“…Figure 7 shows the mean fold increases in frequency, amplitude, rise time and decay time caused by noradrenaline. As reported previously (Braga et al 2004; Kaneko et al 2008; Sekiguchi et al 2009; Miyajima et al 2010), the effect of noradrenaline on event frequency was pronounced, whereas other parameters showed little change in all the groups. One‐way ANOVA revealed that there were no statistically significant differences among the four groups in frequency ( F 3,38 = 0.84, P = 0.48), amplitude ( F 3,38 = 1.324, P = 0.28), rise time ( F 3,38 = 2.07, P = 0.12) and decay time ( F 3,38 = 2.16, P = 0.11).…”

Section: Resultssupporting

confidence: 88%

“…The threshold amplitude for current detection was 5 pA, and the times required for a 37% decay and a 10–90% rise in the response were taken as the decay and rise times, respectively. The Kolmogorov–Smirnov (KS) test was used for probability distribution comparisons between two data groups (Miyajima et al 2010).…”

Section: Methodsmentioning

confidence: 99%

“…In adult rats, stress such as restraint or footshock induces the release of noradrenaline into the amygdala (Tanaka et al 1983; Galvez et al 1996). The GABAergic system in the basolateral amygdala (BLA) is known to be activated by this catecholamine (Braga et al 2004; Kaneko et al 2008; Sekiguchi et al 2009; Miyajima et al 2010). Namely, in the adult rat and mouse BLA, noradrenaline excites unidentified GABAergic neurons via α1‐adrenoceptors, resulting in increased action potential firing.…”

Section: Introductionmentioning

confidence: 99%

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Adult‐like action potential properties and abundant GABAergic synaptic responses in amygdala neurons from newborn marmosets

Yamada

Miyajima

Ishibashi

et al. 2012

The Journal of Physiology

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Key points• The amygdala plays an important role in the processing of emotional events, but little is known about the postnatal development of electrophysiological properties of amygdala neurons.• We performed slice patch-clamp recordings from basolateral amygdala (BLA) pyramidal neurons in the developing and adult common marmoset (Callithrix jacchus).• The marmoset is a non-human primate that has been increasingly used as an animal model of human disease, but no previous studies have used brain slice patch-clamp techniques in marmosets.• We found that BLA pyramidal neurons from newborn marmosets possess relatively mature action potentials and receive vigorous GABAergic synaptic inputs, and that marmoset BLA neurons acquire adult-like electrophysiological properties within the first 4 weeks of life.• Our study provides novel information on several features of postnatal development of BLA pyramidal neurons in marmosets.Abstract The amygdala plays an important role in the processing of emotional events. This information processing is altered by development, but little is known about the development of electrophysiological properties of neurons in the amygdala. We studied the postnatal development of electrophysiological properties of neurons in the basolateral amygdala (BLA) of the common marmoset (Callithrix jacchus). Whole-cell patch-clamp recordings were obtained from BLA pyramidal neurons in brain slices prepared from developing and adult marmosets, and electrophysiological properties known to change during development in rats were analysed. Two passive electrical properties of the neuronal membrane -the input resistance (R in ) and the membrane time constant (τ) -significantly decreased with postnatal development. In contrast, the action potential only showed a slight decrease in duration during the first month of life, whereas the amplitude did not change after birth. Passive electrical properties and action potentials in neurons of 4-week-old marmosets were similar to those in neurons of 4-year-old marmosets. The development of the action potential duration was not correlated with the development of R in or τ, whereas the development of R in and τ was correlated with each other. Abundant spontaneous and noradrenaline-induced GABAergic currents were present immediately after birth and did not change during postnatal development. These results suggest that newborn infant marmoset BLA pyramidal neurons possess relatively mature action potentials and receive vigorous GABAergic

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Section: Resultssupporting

confidence: 88%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Adult‐like action potential properties and abundant GABAergic synaptic responses in amygdala neurons from newborn marmosets

Yamada

Miyajima

Ishibashi

et al. 2012

The Journal of Physiology

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“…The BLA receives extensive noradrenergic (NA; norepinephrine) innervation from the locus coeruleus (LC) and nucleus of the solitary tract (NTS; Pitkanen, ; Williams et al, ), which synapse onto GABAergic interneurons (Li et al, ). NA released from LC terminals activates three distinct classes of adrenoreceptors (AR; α1, α2, and ß AR) that have multiple subtypes and appear to be more potent modulators of GABAergic inhibitory synaptic transmission than DA (Miyajima et al, ). Although no study has yet anatomically identified the type of interneuron to which the receptor subunits are localized in the BLA, electrophysiological evidence indicates that α1 and α2 AR activation depolarizes SOM‐ and CCK‐positive interneurons, resulting in action potential firing and enhanced inhibitory synaptic transmission (Braga et al, ; Buffalari and Grace, ; Kaneko et al, ).…”

Section: Modulation Of Gabaa Receptor‐mediated Inhibitory Synaptic Trmentioning

confidence: 99%

The basolateral amygdala γ‐aminobutyric acidergic system in health and disease

Prager

Bergstrom

Wynn

et al. 2015

J of Neuroscience Research

143

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The brain comprises an excitatory/inhibitory neuronal network that maintains a finely tuned balance of activity critical for normal functioning. Excitatory activity in the basolateral amygdala (BLA), a brain region that plays a central role in emotion and motivational processing, is tightly regulated by a relatively small population of gamma-aminobutyric acid (GABA) inhibitory neurons. Disruption in GABAergic inhibition in the BLA can occur when there is a loss of local GABAergic interneurons, alterations in GABAA receptor activation, or dysregulation of mechanisms that modulate BLA GABAergic inhibition. Disruptions in GABAergic control of the BLA emerge during development, in aging populations, or after a trauma, ultimately resulting in hyperexcitability. BLA hyperexcitability manifests behaviorally as an increase in anxiety, emotional dysregulation, or the development of seizure activity. This article reviews the anatomy, development, and physiology of the GABAergic system in the BLA, and circuits that modulate GABAergic inhibition, including the dopaminergic, serotonergic, noradrenergic, and cholinergic systems. We highlight how alterations in various neurotransmitter receptors, including the acid sensing ion channel 1a (ASIC1a), cannabinoid receptor 1 (CB1), and glutamate receptor subtypes, expressed on BLA interneurons, modulate GABAergic transmission and how defects of these systems affects inhibitory tonus within the BLA. Finally, we discuss alterations in the BLA GABAergic system in neurodevelopmental (autism/Fragile X syndrome) and neurodegenerative (Alzheimer’s disease) diseases, and after the development of epilepsy, anxiety, and traumatic brain injury. A more complete understanding of the intrinsic excitatory/inhibitory circuit balance of the amygdala and how imbalances in inhibitory control contribute to excessive BLA excitability will guide the development of novel therapeutic approaches in neuropsychiatric diseases.

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“…), norepinephrine (Miyajima et al . ; Silberman, Ariwodola & Weiner ) and serotonin (Hofelmann et al . ), may also be involved in re‐establishing normal levels of basal GABAergic transmission after 2–3 weeks of intermittent ethanol exposure.…”

Section: Discussionmentioning

confidence: 99%

Chronic alcohol exposure disrupts CB₁ regulation of GABAergic transmission in the rat basolateral amygdala

et al. 2016

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The basolateral nucleus of the amygdala (BLA) is critical to the pathophysiology of anxiety-driven alcohol drinking and relapse. The endogenous cannabinoid/type 1 cannabinoid receptor (eCB/CB1) system curbs BLA-driven anxiety and stress responses via a retrograde negative feedback system that inhibits neurotransmitter release, and BLA CB1 activation reduces GABA release and drives anxiogenesis. Additionally, decreased amygdala CB1 is observed in abstinent alcoholic patients and ethanol withdrawn rats. Here we investigated the potential disruption of eCB/CB1 signaling on GABAergic transmission in BLA pyramidal neurons of rats exposed to 2–3 weeks intermittent ethanol. In the naïve rat BLA, the CB1 agonist WIN 55,212-2 (WIN) decreased GABA release and this effect was prevented by the CB1 antagonist AM251. AM251 alone increased GABA release via a mechanism requiring postsynaptic calcium-dependent activity. This retrograde tonic eCB/CB1 signaling was diminished in chronic ethanol exposed rats, suggesting a functional impairment of the eCB/CB1 system. In contrast, acute ethanol increased GABAergic transmission similarly in naïve and chronic ethanol exposed rats, via both pre- and postsynaptic mechanisms. Notably, CB1 activation impaired ethanol’s facilitation of GABAergic transmission across both groups, but the AM251- and ethanol-induced facilitation of GABA release were additive, suggesting independent presynaptic sites of action. Collectively, the present findings highlight a critical CB1 influence on BLA GABAergic transmission that is dysregulated by chronic ethanol exposure and thus, may contribute to the alcohol dependent state.

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Noradrenaline-induced spontaneous inhibitory postsynaptic currents in mouse basolateral nucleus of amygdala pyramidal neurons: Comparison with dopamine-induced currents

Cited by 4 publications

References 0 publications

Adult‐like action potential properties and abundant GABAergic synaptic responses in amygdala neurons from newborn marmosets

Adult‐like action potential properties and abundant GABAergic synaptic responses in amygdala neurons from newborn marmosets

The basolateral amygdala γ‐aminobutyric acidergic system in health and disease

Chronic alcohol exposure disrupts CB₁ regulation of GABAergic transmission in the rat basolateral amygdala

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Noradrenaline-induced spontaneous inhibitory postsynaptic currents in mouse basolateral nucleus of amygdala pyramidal neurons: Comparison with dopamine-induced currents

Cited by 4 publications

References 0 publications

Adult‐like action potential properties and abundant GABAergic synaptic responses in amygdala neurons from newborn marmosets

Adult‐like action potential properties and abundant GABAergic synaptic responses in amygdala neurons from newborn marmosets

The basolateral amygdala γ‐aminobutyric acidergic system in health and disease

Chronic alcohol exposure disrupts CB1 regulation of GABAergic transmission in the rat basolateral amygdala

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Chronic alcohol exposure disrupts CB₁ regulation of GABAergic transmission in the rat basolateral amygdala