nor 3′-Demethoxyisoguaiacin from Larrea tridentata Is a Potential Alternative against Multidrug-Resistant Bacteria Associated with Bovine Mastitis

Morales-Ubaldo, Ana Lizet; González-Cortazar, Manasés; Zaragoza-Bastida, Adrián; Meza-Nieto, Martín A.; Valladares-Carranza, Benjamín; Alsayegh, Abdulrahman A.; Batiha, Gaber El‐Saber; Rivero-Pérez, Nallely

doi:10.3390/molecules27113620

Cited by 7 publications

(5 citation statements)

References 83 publications

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“…The active test compounds with inhibition zones and 5,7-dihidroxyflavanone were further investigated to determine their minimal inhibitory concentration (MIC) using a microdilution method ( Morales-Ubaldo et al, 2022 ). Briefly, in 96-well plates, the stock solutions of the extracts were serially diluted twofold in methanol:water (1:9 v / v ) to final concentrations between 200 to 0.195 mg/mL for extracts, 12.5 to 0.006 mg/mL for C1F1 to C1F10 fractions and 6.14 to 0.006 mg/mL for C2F1 to C2F6.…”

Section: Methodsmentioning

confidence: 99%

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Litsea glaucescens Kuth possesses bactericidal activity against Listeria monocytogenes

Gress-Antonio,

Rivero-Perez,

Marquina-Bahena

et al. 2023

PeerJ

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Background Litsea glaucencens Kuth is an aromatic plant used for food seasoning food and in Mexican traditional medicine. Among, L. glaucencens leaves properties, it has proven antibacterial activity which can be used against opportunistic pathogens like Listeria monocytogenes, a foodborne bacteria that is the causal agent of listeriosis, a disease that can be fatal in susceptible individuals. The aim of this work was to investigate the antibacterial activity of L. glaucescens Kuth leaf extracts against L. monocytogenes and to identify its bioactive components. Material and Methods L. glaucences leaves were macerated with four solvents of different polarity (n-hexane, dichloromethane, ethyl acetate, and methanol). To determine the capacity to inhibit bacterial proliferation in vitro, agar diffusion and microdilution methods were used. Next, we determined the minimal bactericidal concentration (MBC). Finally, we determined the ratio of MBC/MIC. Metabolites present in the active methanolic extract from L. glaucescens Kuth (LgMeOH) were purified by normal-phase open column chromatography. The structure of the antibacterial metabolite was determined using nuclear magnetic resonance (1H, 13C, COSY, HSQC) and by comparison with known compounds. Results The LgMeOH extract was used to purify the compound responsible for the observed antimicrobial activity. This compound was identified as 5,7-dihydroxyflavanone (pinocembrin) by analysis of its spectroscopic data and comparison with those described. The MIC and MBC values obtained for pinocembrin were 0.68 mg/mL, and the ratio MBC/MIC for both LgMeOH and pinocembrin was one, which indicates bactericidal activity. Conclusion L. glaucences Kuth leaves and its metabolite pinocembrin can be used to treat listeriosis due the bactericidal activity against L. monocytogenes.

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Section: Methodsmentioning

confidence: 99%

“…The minimum bactericidal concentration (MBC) was performed according to Morales-Ubaldo et al (2022) with slight modifications. Briefly, 10 µL of each sample from the plates where there was no INT color change were added to the agar plates inoculated with a fresh cell suspension (10 8 CFU).…”

Section: Methodsmentioning

confidence: 99%

Litsea glaucescens Kuth possesses bactericidal activity against Listeria monocytogenes

Gress-Antonio,

Rivero-Perez,

Marquina-Bahena

et al. 2023

PeerJ

Self Cite

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“…Morale-Ubaldo et al [ 295 ] isolated and characterized the antibacterial compounds from Larrea tridentate through HPLC techniques against multidrug-resistant bacteria associated with bovine mastitis. The results indicated that nor-3 demethoxy isoguaiacin can be used as an alternative treatment for mastitis.…”

Section: Alternative Therapeutic Approaches For Bovine Mastitismentioning

confidence: 99%

Advances in Diagnostic Approaches and Therapeutic Management in Bovine Mastitis

Kour

Sharma

Balaji

et al. 2023

Veterinary Sciences

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Mastitis causes huge economic losses to dairy farmers worldwide, which largely negatively affects the quality and quantity of milk. Mastitis decreases overall milk production, degrades milk quality, increases milk losses because of milk being discarded, and increases overall production costs due to higher treatment and labour costs and premature culling. This review article discusses mastitis with respect to its clinical epidemiology, the pathogens involved, economic losses, and basic and advanced diagnostic tools that have been used in recent times to diagnose mastitis effectively. There is an increasing focus on the application of novel therapeutic approaches as an alternative to conventional antibiotic therapy because of the decreasing effectiveness of antibiotics, emergence of antibiotic-resistant bacteria, issue of antibiotic residues in the food chain, food safety issues, and environmental impacts. This article also discussed nanoparticles’/chitosan’s roles in antibiotic-resistant strains and ethno-veterinary practices for mastitis treatment in dairy cattle.

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“…It has been reported that carvacrol, hexadecanoic acid, NDGA, and thymol are compounds with bactericidal activity present, as applicable, in the extracts of L. tridentata and O. vulgare obtained with polar organic solvents 3,10,30,31 . It should be noted that, although mechanisms of antibacterial activity of these compounds against most bacteria have not been proposed, the bactericidal capacity has been associated with the alteration of the cytoplasmic membrane 32 .…”

Section: Chemical Composition and Functional Groupsmentioning

confidence: 99%

Minimum inhibitory but maximum non-hemolytic concentration of Larrea tridentata and Origanum vulgare extracts

Alonso Montemayor,

Dávila Medina,

Zugasti Cruz

et al. 2023

Afinidad

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Larrea tridentata and Origanum vulgare extracts are used in popular medicine to obtain antibacterial compounds to combat bacterial infections. Although the antibacterial activity of L. tridentata and O. vulgare extracts has been reported, hemolytic activity needs to be studied for therapeutic application. This work hypothesized minimum inhibitory but non-hemolytic concentration (MIC-NH) for L. tridentata and O. vulgare extracts. Thus, this study aimed to obtain non-hemolytic crude extracts with antibacterial activity. It was estimated that the MIC-NH of L. tridentata and O. vulgare extracts against the Staphylococcus aureus strain, an important skin pathogen bacterium, were 100 and 75 μg mL-1, respectively. Thus, these in vitro-tested extracts prevented the S. aureus strain growth without provoking hemolysis if the extract concentration was less or equal to the MIC-NH. At the same time, it was determined by gas chromatography coupled with mass spectroscopy that bis-ethylhexyl-phthalate, catechol, encecalinol, isopropyl cinnamate, phenol, guaiacine, and NDGA were antibacterial compounds in the L. tridentata extracts. Meanwhile, for O. vulgare extracts, these compounds were cymene, terpinene, thymol, carvacrol, cimendiol, hexadecanoic acid, bis-ethylhexyl-phthalate, and sitosterol. The findings of this research work must encourage the development of potential biomaterial coatings to replace conventional ones.

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nor 3′-Demethoxyisoguaiacin from Larrea tridentata Is a Potential Alternative against Multidrug-Resistant Bacteria Associated with Bovine Mastitis

Cited by 7 publications

References 83 publications

Litsea glaucescens Kuth possesses bactericidal activity against Listeria monocytogenes

Litsea glaucescens Kuth possesses bactericidal activity against Listeria monocytogenes

Advances in Diagnostic Approaches and Therapeutic Management in Bovine Mastitis

Minimum inhibitory but maximum non-hemolytic concentration of Larrea tridentata and Origanum vulgare extracts

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