Maitake mushroom was cultivated on logs and in sawdust substrate. Comparisons of proximate compositions, content of free amino acid, 5¢ ¢ ¢ ¢-nucleotides and vitamin D 2 were conducted. Effects of the log and sawdust substrate compositions on the mushroom composition were also examined. Protein and ash in sawdust mushroom were significantly higher than that in the log mushroom. Protein content of fruit body cultivated on sawdust substrate was closely related to the content of the substrate. This fact well explained the difference in protein and ash contents between mushroom cultivated on log or sawdust substrate. Free amino acid content as MSG-like and sweetness components, and 5¢ ¢ ¢ ¢-GMP were significantly higher in log mushroom. The content of vitamin D 2 was appreciably higher in sawdust mushroom than that in log mushroom.Keywords: Maitake mushroom, log, sawdust substrate, proximate composition, free amino acid, 5¢-GMP, vitamin D 2 Maitake mushroom, G. frondosa (Fr.) S. F. Gray, is a white-rot fungus found in the wild on dead stumps of broad leaf trees such as oak (Quercus mongolica) and chestnut (Castanea crenata) (Imazeki & Hongo, 1985). Almost 40,000 metric tons of the cultivated mushroom was consumed in 1999 in Japan being the fourth largest production (Ohashi, 2000).Numerous investigations have been reported on the components of G. frondosa (Yokokawa 1980;Kurasawa et al., 1982;Sato et al., 1985;Takeuchi et al., 1985;Muratsubaki et al., 1986;Kawai et al., 1990;Sekizawa et al., 1992;Yoshida et al., 1996;Ohnishi et al., 1996;Shindo et al., 1999). It is interesting that consumption of the mushroom is a way to assimilate antitumor (Ohno et al., 1985;Zhuang et al., 1994;Kubo et al., 1994), antidiabetes and antihyperliposis (Kubo et al., 1994(Kubo et al., , 1997, blood pressure and body weight depressive substances (Ohtsuru et al., 1999). Furthermore, Seguchi et al. (2001) added G. frondosa powder to improve the quality of bread.G. frondosa is mainly cultivated on the sawdust of broad leaf trees supplementing the source of nutrients such as rice or wheat bran. This method makes it possible to cultivate on a large scale and to harvest all year round. However, it has been said that sawdust G. frondosa is less tasty than log G. frondosa. A few investigations have been reported on the comparison of chemical compositions of mushroom cultivated on logs and sawdust substrates (Aoyagi et al., 1993;Kawai et al., 1994;Sasaki et al., 1995). However, no study is found on a comparison of the taste components between the sawdust mushroom and log mushroom.Previously, we compared the chemical and free amino acid composition of P. nameko cultivated on log and in sawdust substrate beds (Yamasaki & Tabata, 2002).In this paper, we compared the proximate compositions, free amino acid, 5¢-nucleotides, vitamin D 2 of G. frondosa cultivated on logs and sawdust substrates and their relation to the composition of the substrates.
Materials and MethodsThe strain of G. frondosa was purchased from Onuki Kinjin Co., Utsunomiya-shi. The mu...