Nonvolatile metabolism in postharvest tea (Camellia sinensis L.) leaves: Effects of different withering treatments on nonvolatile metabolites, gene expression levels, and enzyme activity

Yu, Xiao Fang; Li, Yuchuan; He, Chang; Zhou, Ji‐Xun; Chen, Yuqiong; Yu, Zhi; Wang, Pu; Ni, Dejiang

doi:10.1016/j.foodchem.2020.126992

Cited by 50 publications

(45 citation statements)

References 34 publications

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“…Omics technologies have brought about a significant breakthrough in the knowledge about plant responses to LTs [3][4][5][6][7]. In the present study, global differences in mRNAs caused by mild and moderate chilling were systematically identified and analyzed in China's most important banana cultivar with an annual yield of several million tons.…”

Section: Mild and Moderate Chilling Results In Substantial Changes Ofmentioning

confidence: 99%

“…Low temperature (LT) is one of the major environmental factors which not only limit the productivity and the geographical distribution of crops but also cause significant losses [1,2]. In recent years, modern high-throughput expression profiling techniques, such as genomics, transcriptomics, and proteomics, have provided useful tools for better understanding the roles of genes when responding to LT stresses [3][4][5][6][7]. However, most of these studies reported about the effects of severe LT (≤10 • C) on temperate [5,[7][8][9], tropical, and subtropical [3,4,10] plants.…”

Section: Introductionmentioning

confidence: 99%

“…In recent years, modern high-throughput expression profiling techniques, such as genomics, transcriptomics, and proteomics, have provided useful tools for better understanding the roles of genes when responding to LT stresses [3][4][5][6][7]. However, most of these studies reported about the effects of severe LT (≤10 • C) on temperate [5,[7][8][9], tropical, and subtropical [3,4,10] plants. Thus, information about the gene expression dynamics of tropical crops in response to mild (>15 • C) and moderate chilling (10-15 • C) is scarce [11,12], and the underlying molecular background is not well understood.…”

Section: Introductionmentioning

confidence: 99%

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Acceleration of Carbon Fixation in Chilling-Sensitive Banana under Mild and Moderate Chilling Stresses

Liu

Takáč

Chen

et al. 2020

IJMS

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Banana is one of the most important food and fruit crops in the world and its growth is ceasing at 10–17 °C. However, the mechanisms determining the tolerance of banana to mild (>15 °C) and moderate chilling (10–15 °C) are elusive. Furthermore, the biochemical controls over the photosynthesis in tropical plant species at low temperatures above 10 °C is not well understood. The purpose of this research was to reveal the response of chilling-sensitive banana to mild (16 °C) and moderate chilling stress (10 °C) at the molecular (transcripts, proteins) and physiological levels. The results showed different transcriptome responses between mild and moderate chilling stresses, especially in pathways of plant hormone signal transduction, ABC transporters, ubiquinone, and other terpenoid-quinone biosynthesis. Interestingly, functions related to carbon fixation were assigned preferentially to upregulated genes/proteins, while photosynthesis and photosynthesis-antenna proteins were downregulated at 10 °C, as revealed by both digital gene expression and proteomic analysis. These results were confirmed by qPCR and immunofluorescence labeling methods. Conclusion: Banana responded to the mild chilling stress dramatically at the molecular level. To compensate for the decreased photosynthesis efficiency caused by mild and moderate chilling stresses, banana accelerated its carbon fixation, mainly through upregulation of phosphoenolpyruvate carboxylases.

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Section: Mild and Moderate Chilling Results In Substantial Changes Ofmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Acceleration of Carbon Fixation in Chilling-Sensitive Banana under Mild and Moderate Chilling Stresses

Liu

Takáč

Chen

et al. 2020

IJMS

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“…Untargeted nonvolatile constituents in black tea were determined [22]. In short, 0.15 g of each sample was weighed and added to 7.5 mL of a 75% methanol-water solution and 0.15 mL of etophylline (250 g/mL) as an internal standard.…”

Section: Determination Of the Main Components Of Black Teamentioning

confidence: 99%

Different Withering Times Affect Sensory Qualities, Chemical Components, and Nutritional Characteristics of Black Tea

Ntezimana

et al. 2021

Foods

Self Cite

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The present study emphasizes the effect of withering time set at 4 ± 0.5 h (WT4), 6 ± 0.5 h (WT6), 8 ± 0.5 h (WT8), 10 ± 0.5 h (WT10), and 12 ± 0.5 h (WT12) on the sensory qualities, chemical components, and nutritional characteristics of black tea. The sensory evaluation revealed high total quality scores at WT8 and WT10. Polysaccharides, amino acids, and soluble sugars significantly increased with an increase in withering time, and an apparent peak value was obtained at WT10. However, polyphenols, flavonoids, glycosides, organic acids, catechins, alkanoids, and theaflavins decreased with an increase in withering time. With an increase in withering time, the content of aromatic substances showed a trend of increasing first and then decreasing. The peaks of alcohols, aldehydes, and acids appeared at 10 ± 0.5 h, 10 ± 0.5 h, and 8 ± 0.5 h, respectively. The content of esters, ketones, and hydrocarbons showed a downward trend with an increase in withering time. Aroma analysis revealed that withering time could not exceed 10 ± 0.5 h. Black tea withered up to WT10 showed enhanced inhibition of α-glucosidase and α-amylase activity with good sensorial attributes. Glucose uptake inhibition capacity increased up 6 ± 0.5 h and then decreased, while antioxidant capacity decreased with an increase in withering time. The overall results show that the 8 ± 0.5 h to 10 ± 0.5 h withering time could improve black tea quality and nutritional characteristics.

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“…PCA and PLS are used to discriminate and analyze the error value and signi cant difference. A cluster analysis of the identi ed metabolites was conducted after auto-scaling (mean-centered and divided by the standard deviation) by using MultiExperiment Viewer [21].…”

Section: Data Processing and Statistics Analysismentioning

confidence: 99%

Exploring the adaptive mechanism of Camellia limonia in karst high calcium environment via an integrative analysis of metabolomics and metagenomics

Liu

Huang

et al. 2021

Preprint

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Background: Yellow Camellia is a kind of rare plant with high economic and medicinal value. It is known as the "giant panda" of the botanical world. Camellia group of plants (Camellia Sect. Chrysantha Chang) is the only golden flower of Theaceae. Compared with Camellia nitidissima, Camellia limonia grows in karst areas, where the soil has the characteristic of high calcium content. However, there are few studies about the Camellia limonia in karst soil environment and the adaptation mechanism is no clear. Results: In this study, we found that under high calcium treatment, the chlorophyll content and leaf areas of Camellia limonia increased, while those of Camellia nitidissima decreased. The photosynthetic efficiency of Camellia limonia was more stable and higher than Camellia nitidissima. Compared with Camellia nitidissima, the conductance was larger and the degree of leaf shrinkage was smaller in Camellia limonia. The metabolomics analysis showed that the kaempferol-3-o-rutinoside, tyrosol, 6-o-methyldeacetylisoipecoside and (r)-mandelic acid are the main differently metabolic compounds . The results of karst high calcium soil metagenomics showed that microbacterium-testaceum, intrasporangium-calvum and rubrivivax-gelatinosus significantly changed. Through metabonomics and metagenomics integrative analysis, flavone and flavonol biosynthesis is suggested to be the main regulation pathway, which is regulated by apigenin, kaempferol, astragalin, isoquercitrin metabolites and TT7, UGT78D1, UGT78D2 genes. This metabolic pathway involves the synthesis of flavonoids. Flavonoids have the functions in drought and salt resistance, which play an important role in the adaptation of Camellia limonia in karst high calcium environment.Conclusion: This omics study identified key regulation metabolites and genes for Camellia and provided important basis for the adaptive mechanism of plants to adapt to the high-calcium environment and the protection of Camellia species.

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Nonvolatile metabolism in postharvest tea (Camellia sinensis L.) leaves: Effects of different withering treatments on nonvolatile metabolites, gene expression levels, and enzyme activity

Cited by 50 publications

References 34 publications

Acceleration of Carbon Fixation in Chilling-Sensitive Banana under Mild and Moderate Chilling Stresses

Acceleration of Carbon Fixation in Chilling-Sensitive Banana under Mild and Moderate Chilling Stresses

Different Withering Times Affect Sensory Qualities, Chemical Components, and Nutritional Characteristics of Black Tea

Exploring the adaptive mechanism of Camellia limonia in karst high calcium environment via an integrative analysis of metabolomics and metagenomics

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