Nontuberculous Mycobacteria in Respiratory Tract Infections, Eastern Asia

Simons, Sami O.; Ingen, Jakko van; Hsueh, Po‐Ren; Hùng, Nguyễn Văn; Dekhuijzen, P.N.R.; Boeree, Martin J.; Soolingen, Dick van

doi:10.3201/eid1703.100604

Cited by 114 publications

(80 citation statements)

References 29 publications

(51 reference statements)

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“…The high isolation frequency of RGM, particularly M. abscessus, was also noted in previous studies focusing on this region [19][20][21]. The reason for the high isolation rate of RGM in Asian countries remains unclear, although geographical or climatic factors, host and laboratory factors have been suggested [20]. An important reason to perform studies on the geographical distribution of NTM is to help identify factors associated with differences in worldwide isolation patterns of specific species.…”

Section: Discussionmentioning

confidence: 70%

The geographic diversity of nontuberculous mycobacteria isolated from pulmonary samples: an NTM-NET collaborative study

Hoefsloot¹,

Andréjak²,

Ängeby³

et al. 2013

Eur Respir J

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A significant knowledge gap exists concerning the geographical distribution of nontuberculous mycobacteria (NTM) isolation worldwide.To provide a snapshot of NTM species distribution, global partners in the NTM-Network European Trials Group (NET) framework (www.ntm-net.org), a branch of the Tuberculosis Network European Trials Group (TB-NET), provided identification results of the total number of patients in 2008 in whom NTM were isolated from pulmonary samples. From these data, we visualised the relative distribution of the different NTM found per continent and per country.We received species identification data for 20 182 patients, from 62 laboratories in 30 countries across six continents. 91 different NTM species were isolated. Mycobacterium avium complex (MAC) bacteria predominated in most countries, followed by M. gordonae and M. xenopi. Important differences in geographical distribution of MAC species as well as M. xenopi, M. kansasii and rapid-growing mycobacteria were observed.This snapshot demonstrates that the species distribution among NTM isolates from pulmonary specimens in the year 2008 differed by continent and differed by country within these continents. These differences in species distribution may partly determine the frequency and manifestations of pulmonary NTM disease in each geographical location. @ERSpublications Species distribution among nontuberculous mycobacteria isolates from pulmonary specimens is geographically diverse

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Section: Discussionmentioning

confidence: 70%

The geographic diversity of nontuberculous mycobacteria isolated from pulmonary samples: an NTM-NET collaborative study

Hoefsloot¹,

Andréjak²,

Ängeby³

et al. 2013

Eur Respir J

Self Cite

707

532

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“…For example, in most of Asia and New Zealand after MAC, RGM have generally been the most frequently identified species but India reports no PNTM due to MAC and attributes 40% to M. fortuitum and 33% to M. kansasii [34]. In Britain, MAC (43%), Mycobacterium malmoense (14%) and M. kansasii (13%) were the most frequently isolated species from respiratory specimens [35].…”

Section: Epidemiologymentioning

confidence: 99%

Pulmonary disease caused by nontuberculous mycobacteria

Weiss

Glassroth²

2012

Expert Review of Respiratory Medicine

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“…In contrast, the clarithromycin MIC values of 33 of 36 M. massiliense isolates were Յ2 g/ml when the broth susceptibility test results were read on day 5; however, 11 (33.3%) of the 33 isolates exhibited clarithromycin MICs of Ͼ16 (Ն32) g/ml during the 14-day observation. Previous studies have shown that rapidly growing mycobacteria, especially those of the M. abscessus complex, are the most common clinical isolates of mycobacteria and are a major cause of pulmonary disease due to nontuberculous mycobacteria (NTM) in Asia (2,3,(18)(19)(20). Saleeb et al reported that MALDI-TOF MS can be incorporated into the work flow of the microbiology laboratory for rapid and accurate identification of most species of mycobacteria (16).…”

mentioning

confidence: 99%

Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry Can Accurately Differentiate between Mycobacterium masilliense (M. abscessus subspecies bolletti) and M. abscessus ( Sensu Stricto )

et al. 2013

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Among 36 Mycobacterium masilliense and 22 M. abscessus isolates identified by erm(41) PCR and sequencing analysis of rpoB and 23S rRNA genes, the rate of accurate differentiation between these two subspecies was 100% by cluster analysis of spectra generated by Bruker Biotyper matrix-assisted laser desorption ionization-time of flight mass spectrometry. Mycobacterium abscessus complex, a rapidly growing mycobacterium, is the cause of an increasing number of community-and health care-associated infections in humans (1). The isolation of M. abscessus complex from patients with various clinical infections has been reported in many countries, including Taiwan (2-7). The M. abscessus complex comprises three closely related subspecies, namely, M. massiliense, M. bolletii, and M. abscessus (sensu stricto) (4,5,(7)(8)(9). Identification of M. abscessus complex members to the species level depends on sequencing analysis of several genes, including the erm(41) gene, the 23S rRNA gene, and several housekeeping genes (e.g., rpoB and hsp65) (7,(9)(10)(11)(12). A previous report found that erm(41) PCR can differentiate M. massiliense from M. abscessus and M. bolletii but that sequencing analysis of rpoB and hsp65 was less reliable at differentiating between the two (9). M. abscessus subsp. bolletti is now the recommended taxonomic name for M. massiliense (4, 12). Differences of in vitro susceptibilities to clarithromycin between M. massiliense (M. abscessus subsp. Bolletti) and M. abscessus (sensu stricto) isolates and of treatment response rates of lung diseases caused by these two species with clarithromycin-based antibiotic therapy have been reported (7-12). However, the use of molecular methods to differentiate among these subspecies is not possible in many routine microbiology laboratories.The use of matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) is relatively new in the field of microbiology for species identification of yeasts and bacteria, including mycobacteria (13-17). Although MALDI-TOF MS has been shown to be a highly accurate method for identifying M. abscessus complex isolates to the species level, this method has not been shown to be able to differentiate between subspecies of the M. abscessus complex, i.e., M. massiliense (M. abscessus subsp. bolletti) and M. abscessus (sensu stricto) (15,16).In this study, we evaluated a total of 58 isolates of the M. abscessus complex obtained from various clinical specimens from patients treated during the period from January 2011 to December 2012 at the National Taiwan University Hospital, a 2,900-bed tertiary-care medical center in northern Taiwan. The isolates were presumptively identified as M. abscessus complex based on conventional biochemical methods as previously described (4-6).These isolates were further identified to the subspecies level by sequencing the erm(41) gene and by performing sequence analysis techniques targeting the rpoB and 23S rRNA genes. The following primer pairs were used: ermF (5=-GAC CGG GGC CTT CTT C...

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Nontuberculous Mycobacteria in Respiratory Tract Infections, Eastern Asia

Cited by 114 publications

References 29 publications

The geographic diversity of nontuberculous mycobacteria isolated from pulmonary samples: an NTM-NET collaborative study

The geographic diversity of nontuberculous mycobacteria isolated from pulmonary samples: an NTM-NET collaborative study

Pulmonary disease caused by nontuberculous mycobacteria

Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry Can Accurately Differentiate between Mycobacterium masilliense (M. abscessus subspecies bolletti) and M. abscessus ( Sensu Stricto )

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