Nonthermal Microwave Radiations Affect the Hypersensitive Response of Tobacco to Tobacco Mosaic Virus

Betti, Lucietta; Trebbi, Grazia; Lazzarato, Lisa; Brizzi, Maurizio; Calzoni, G. L.; Marinelli, Francesco; Nani, Daniele; Borghini, Francesco

doi:10.1089/acm.2004.10.947

Cited by 8 publications

(9 citation statements)

References 50 publications

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“…In addition, Betti (2004) reported a non-thermal effect of microwaves against plants and viruses at a sublethal temperature, and Wu and Yao (2010) confirmed visible changes of bacteria and fungi after microwave heat treatment by ESEM. Hence, in this study non-thermal effects were investigated by studying the morphological changes and SFs with and without microwaves at the same temperature.…”

Section: Effective Temperaturementioning

confidence: 97%

“…Meanwhile, there has been research demonstrating additional effects, beyond the purely thermal mode of inactivation. For example, distortion of membrane structure and function (Phelan et al, 1994), altered enzyme activity (Dreyfuss and Chipley, 1980), disruption of weak bonds (Betti et al, 2004), increased release of various substances (Woo et al, 2000;Celandroni et al, 2004;Campanha et al, 2007), and increased ionic strength due to an increased current within cells (Watanabe et al, 2000) have all been reported. However, all of the aforementioned research was conducted in the liquid, solid, or aqueous phase.…”

Section: Introductionmentioning

confidence: 99%

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Microwave-irradiation-assisted HVAC Filtration for Inactivation of Viral Aerosols

Woo

Grippin

et al. 2012

Aerosol Air Qual. Res.

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Inactivation of collected viral aerosols is important for preventing a filter medium's serving as a fomite. The focus of this study was to evaluate the inactivation efficiency (IE) achieved through filtration coupled with microwave irradiation. MS2 aerosolized through a Collison nebulizer was fed into the system and collected onto the filter. For in-flight microwave decontamination, microwave irradiation was applied to an HVAC (heating, ventilation and air conditioning) filter supported on a SiC disk for three cycles of selected irradiation times per 10 min (i.e., 1, 2.5, 5, and 10 min/10 min) at power levels ranging from 125 W to 375 W. The survival fraction (SF) on the substrate and the IE through the entire system were investigated to determine the efficacy of this approach. SF decreased and IE increased as microwave power level was increased (p = 0.02 and p < 0.01, respectively) or the application time was extended (p = 0.03 and p < 0.01, respectively). Both measures changed sharply above a threshold temperature of around 90°C and reached 2 logs at 116 and 109°C, respectively. The log SF and IE of -2.59 and 3.62, respectively, were observed when the operating condition of 375 W for 10 min/cycle was used and the SiC disk facilitated microwave absorption. When a quartz frit was used as a support instead of the SiC disk, log inactivation efficiencies of 0.8, 1.0, and 1.3 were measured at relative humidities of 30%, 60% and 90%, respectively, under the same irradiation conditions. Relative humidity is a significant parameter from 50-80°C (p = 0.01). The results demonstrate that microwave-assisted filtration systems can be used as an effective means for inactivating viruses.

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Section: Effective Temperaturementioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

Microwave-irradiation-assisted HVAC Filtration for Inactivation of Viral Aerosols

Woo

Grippin

et al. 2012

Aerosol Air Qual. Res.

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“…In addition, Betti et al (2004) reported a nonthermal effect of microwaves against plants and viruses at a sublethal temperature, and Wu and Yao (2010) confirmed visible changes of bacteria and fungi after microwave heat treatment by environmental scanning EM. Hence, in this study nonthermal effects were investigated by studying morphological changes and SFs with and without microwaves at the same temperature.…”

Section: Effective Temperaturementioning

confidence: 83%

Evaluation of the Performance of Iodine-Treated Biocidal Filters Under the Influence of Environmental Parameters

Woo¹,

Li²,

Wu³

2013

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“…High temperatures can cause denaturation and cell membrane damage [34], as well as alter enzymatic activities by disrupting weak bonds in active protein forms [35]. Moreover, MW irradiation exerts nonthermal effects on cell viability by interfering with cell signaling pathways, generating current within cells [36], and producing reactive oxygen species such as % OH [37]. Our previous studies indicated that a significant difference between MW irradiation and sole thermal treatment (Support information, Fig.…”

Section: Bioaerosol Inactivation Performance By Mw Irradiationmentioning

confidence: 97%

“…Similar finding was observed by Wu et al [8] that the microwave-irradiated P. fluorescens cells apparently had damaged membrane envelope as observed in the SEM images. Previous researches showed that MW can alter enzymatic activities by disrupting weak bonds in active protein forms [38], and produce reactive oxygen species (such as % OH) [37], which was used to damage membrane envelope. The SEM images of E. coli cells before and after MW irradiation ( Fig.…”

Section: Bioaerosol Inactivation Performance By Mw Irradiationmentioning

confidence: 99%

Microwave-induced release and degradation of airborne endotoxins from Escherichia coli bioaerosol

WANG

Zhang

Liu

2019

Journal of Hazardous Materials

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Endotoxins are widely distributed toxins in the outer cell-wall membranes of Gram-negative bacteria and other microorganisms. Chronic exposure to endotoxins can induce and exacerbate airway symptoms and diseases. However, the release and degradation of airborne endotoxins from bioaerosol by microwave (MW) irradiation have not yet been reported. This study investigated the distribution and fate of airborne endotoxins during MW irradiation process, as well as the kinetics and thermodynamics of the degradation of airborne endotoxins. Results showed that MW irradiation induced cell lysis, thus considerably increasing the proportion of cells with ruptured membranes. Furthermore, MW irradiation changed the distribution of airborne endotoxins, sharply decreased the concentration of bound endotoxins from 230 EU/m 3 to 68 EU/m 3 , and increased the concentration of free endotoxins from 21 EU/m 3 to 122 EU/m 3 . These results indicated that MW irradiation released endotoxins from cells into the atmosphere. MW irradiation likely degraded endotoxins by exerting thermal effects, which achieved a total endotoxin removal efficiency of as high as 35%. Endotoxin degradation was a first-order reaction and required the activation energy of 26.3 kJ/mol. Fig. 7. Thermodynamic of airborne endotoxin degradation. (A) Endotoxin removal efficiency under various temperatures. (B) Relationship between the reaction rate constant of endotoxin lnk and temperature 1/T. (Error bar presents standard deviation, n presents numbers of samples, n = 3). C. Wang et al.

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Nonthermal Microwave Radiations Affect the Hypersensitive Response of Tobacco to Tobacco Mosaic Virus

Abstract: Nonthermal microwave radiations are effective on the hypersensitive response of tobacco to tobacco mosaic virus and their low-frequency modulation seems to be more bioactive than the continuous-flux of waves, particularly in the indirect water-mediated treatments.

Cited by 8 publications

References 50 publications

Microwave-irradiation-assisted HVAC Filtration for Inactivation of Viral Aerosols

Microwave-irradiation-assisted HVAC Filtration for Inactivation of Viral Aerosols

Evaluation of the Performance of Iodine-Treated Biocidal Filters Under the Influence of Environmental Parameters

Microwave-induced release and degradation of airborne endotoxins from Escherichia coli bioaerosol

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