Nonthermal Irreversible Electroporation for Tissue Decellularization

Phillips, Mary; Maor, Elad; Rubinsky, Boris

doi:10.1115/1.4001882

Cited by 102 publications

(73 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…NTIRE specifically targets the cell membrane, allowing for the preservation of tissue structural components such as the extracellular matrix, blood vessels, and nerves (Onik and Rubinsky, 2010; Phillips et al , 2010). It can be seen that this holds true for the small intestine as well.…”

Section: Discussionmentioning

confidence: 99%

“…Our first mathematical study has proven that, while limited in range, such a domain of electric fields exists (Davalos et al , 2005). Our subsequent studies have shown that NTIRE can ablate tissue (Edd et al , 2006; Rubinsky, 2007; Rubinsky et al , 2007) while retaining the structural integrity of blood vessels, nerves, and extracellular matrix (Onik et al , 2007; Phillips et al , 2010) and that it is effective in destroying cancer in animal models (Al-Sakere et al , 2007; Ellis et al , 2011). Non-thermal irreversible electroporation involves the insertion of thin needle electrodes around an undesirable tissue or cell mass and the application of brief microsecond-scale electric pulses.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Irreversible electroporation on the small intestine

Phillips

Narayan²,

Padath

et al. 2012

Br J Cancer

View full text Add to dashboard Cite

Background:Non-thermal irreversible electroporation (NTIRE) has recently been conceived as a new minimally invasive ablation method, using microsecond electric fields to produce nanoscale defects in the cell membrane bilayer and induce cell death while keeping all other molecules, including the extracellular matrix, intact. Here, we present the first in vivo study that examines the effects of NTIRE on the small intestine, an organ whose collateral damage is of particular concern in the anticipated use of NTIRE for treatment of abdominal cancers.Methods:A typical NTIRE electrical protocol was applied directly to the rat small intestine and histological analysis was used to examine the effect of NTIRE over time.Results:The application of NTIRE led to complete cell ablation in the targeted tissue, but the animal did not show any physiological effects of the procedure and the intestine showed signs of recovery, developing an epithelial layer 3 days post treatment and regenerating its distinct layers within a week.Conclusion:Our results indicate that this novel procedure can be used for abdominal cancer treatment while minimising collateral damage to adjacent tissues because of the unique ability of the NTIRE ablation method to target the cell membrane.

show abstract

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Irreversible electroporation on the small intestine

Phillips

Narayan²,

Padath

et al. 2012

Br J Cancer

View full text Add to dashboard Cite

show abstract

“…The presence of the micropores causes loss of cell homeostasis and leads to cell death. When NTIRE was applied to rat carotid arteries in vivo , it was shown that the cellular remnants were slowly removed from the tissue over a period of days, leaving an acellular tissue approximately three days after NTIRE treatment [95]. The mechanisms for the cellular removal are not clear.…”

Section: Decellularization Agentsmentioning

confidence: 99%

An overview of tissue and whole organ decellularization processes

Crapo¹,

Gilbert²,

Badylak³

2011

Biomaterials

2,714

2,913

View full text Add to dashboard Cite

Biologic scaffold materials composed of extracellular matrix (ECM) are typically derived by processes that involve decellularization of tissues or organs. Preservation of the complex composition and three-dimensional ultrastructure of the ECM is highly desirable but it is recognized that all methods of decellularization result in disruption of the architecture and potential loss of surface structure and composition. Physical methods and chemical and biologic agents are used in combination to lyse cells, followed by rinsing to remove cell remnants. Effective decellularization methodology is dictated by factors such as tissue density and organization, geometric and biologic properties desired for the end product, and the targeted clinical application. Tissue decellularization with preservation of ECM integrity and bioactivity can be optimized by making educated decisions regarding the agents and techniques utilized during processing. An overview of decellularization methods, their effect upon resulting ECM structure and composition, and recently described perfusion techniques for whole organ decellularization techniques are presented herein.

show abstract

“…9–11 This is a dynamic phenomenon and has been implemented in a variety of biologic systems – most commonly for transfection of cells in vivo or in vitro – and is potentially reversible (i.e., the cells may reconstitute their membrane integrity). However, higher voltages used during electroporation, rather than transiently disrupting the cell membrane, have also been shown to be capable of destroying target cells within a discrete lesion while leaving neighboring cells unaffected.…”

Section: Electroporationmentioning

confidence: 99%