Nontarget-Site Resistance to ALS Inhibitors in Waterhemp (<i>Amaranthus tuberculatus</i>)

Guo, Jiaqi; Riggins, Chance W.; Hausman, Nicholas E.; Hager, Aaron G.; Riechers, Dean E.; Davis, Adam S.; Tranel, Patrick J.

doi:10.1614/ws-d-14-00139.1

Cited by 50 publications

(71 citation statements)

References 25 publications

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“…No mutations were detected at Asp 376 , Arg 377 , and Gly 654 in our populations; however, amino acid substitutions at these latter codons are reported to confer additional types and levels of ALS resistance in other weed species Tranel et al 2016), unreported/novel mutations at different codons in the ALS gene, and non-target site resistance (i.e., a translocation-based mechanism) could be the mechanisms of resistance in the remaining ALS inhibitor-resistant populations detected in Nebraska and Kansas by Werle et al (2016a). Non-target site ALS resistance has been reported in common waterhemp by Guo et al (2015). Our future work includes development of high-throughput assays to characterize ALS mutations in shattercane and johnsongrass populations and to more thoroughly characterize putative structural variation at the 5′ end of the gene, where Ala 122 , Pro 197 , a Twenty-three johnsongrass plants from each population were exposed to imazethapyr, and 23 additional plants were exposed to nicosulfuron.…”

Section: Resultsmentioning

confidence: 99%

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Independent Evolution of Acetolactate Synthase–inhibiting Herbicide Resistance in WeedySorghumPopulations across Common Geographic Regions

et al. 2017

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Traditional breeding has been used to develop grain sorghum germplasm that is tolerant to acetolactate synthase (ALS)-inhibiting herbicides (Inzen Technology, DuPont). Inzen sorghum carries a double mutation in the ALS gene (Val 560 Ile and Trp 574 Leu), which confers high level of tolerance to ALS-inhibiting herbicides. Overreliance on ALS-inhibiting herbicides for weed control during the 1990s resulted in the evolution of ALS inhibitor-resistant shattercane populations in Nebraska. According to a survey conducted in 2013, ALS inhibitor-resistant weedy Sorghum populations persist in Nebraska. The objectives of this research were to determine whether the ALS mutations present in Inzen sorghum were present in the ALS inhibitor-resistant shattercane and johnsongrass populations detected in Nebraska and northern Kansas, and whether these populations evolved ALS resistance independently. Primers specific to the Val 560 and Trp 574 region of the ALS gene were used to screen the populations with PCR. The Trp 574 Leu mutation was present in one ALS inhibitor-resistant johnsongrass population. The Val 560 Ile was detected in three ALS inhibitor-resistant shattercane, one susceptible shattercane, one ALS inhibitor-resistant johnsongrass, and one susceptible johnsongrass population. Moreover, Val 560 Ile was present in resistant and/or susceptible individuals within johnsongrass and shattercane populations that were segregating for ALS resistance, indicating that by itself the Val 560 Ile mutation does not confer resistance to ALS-inhibiting herbicides. None of the populations presented both mutations simultaneously, as does Inzen sorghum. A shattercane population containing the Ser 653 Thr mutation was also detected. This research indicates that the ALS mutations present in Inzen sorghum already exist individually in weedy sorghum populations. Moreover, our results present strong evidence that ALS resistance in these populations evolved independently. Thus, widespread overreliance on ALS-inhibiting herbicides prior to adoption of glyphosate-tolerant crops in the 1990s exerted sufficient selective pressure on shattercane and johnsongrass populations for resistance to evolve multiple times in the Midwest. Finally, a survey of the 5′ portion of the ALS gene in more diverse wild and weedy Sorghum species was hampered by limited coverage in genomic resequencing surveys, suggesting that refined PCR-based methods will be needed to assess SNP variation in this gene region, which includes the Ala 122 , Pro 197 , and Ala 205 codons known to confer ALS resistance in other species. Nomenclature: Acetolactate synthase (ALS)-inhibiting herbicides, johnsongrass, Sorghum halepense (L.) Pers. SORHA, shattercane, Sorghum bicolor (L.) Moench ssp. drummondii (Nees ex Steud.) de Wet ex Davidse SORVU, sorghum, Sorghum bicolor (L.) Moench ssp. Bicolor SORVU

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Section: Resultsmentioning

confidence: 99%

“…The Trp 574 Leu mutation was not detected in all R individuals of J-36 (Table 6), indicating that multiple ALS-resistance mechanisms may be present within this population. Multiple mechanisms of ALS resistance within a population have been reported (Guo et al 2015).…”

Section: Resultsmentioning

confidence: 99%

Independent Evolution of Acetolactate Synthase–inhibiting Herbicide Resistance in WeedySorghumPopulations across Common Geographic Regions

et al. 2017

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“…Additionally, the McLean field was treated with soil-and foliarapplied triazine herbicides for at least seven consecutive years (Hausman et al 2011), which likely increased the selection for triazine-resistant waterhemp. Guo et al (2015) reported that MCR contains both target and non-target-site mechanisms that confer resistance to ALS inhibitors. Reduced waterhemp control with glufosinate 14 DAT was attributed primarily to regrowth of treated plants (Table 4).…”

Section: Resultsmentioning

confidence: 99%

Responses of a Waterhemp (Amaranthus tuberculatus) Population Resistant to HPPD-Inhibiting Herbicides to Foliar-Applied Herbicides

Hausman

Tranel²,

Riechers³

et al. 2016

Weed technol.

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Field and greenhouse experiments were conducted to characterize the response of a waterhemp population from McLean County, IL to foliar-applied 4-hydroxyphenylpyruvate dioxygenase (HPPD) -inhibiting herbicides and determine the population's sensitivity to herbicides from other site-of-action groups. In the field, 10 to 15-cm-tall waterhemp treated with mesotrione at 105 g ai ha À1 , tembotrione at 92 g ai ha À1 , or topromezone at 18 g ai ha À1 had significantly greater biomass (! 10%) 14 d after treatment (DAT) than waterhemp harvested the day of herbicide application, indicating growth had occurred following herbicide application. Waterhemp growth stage at the time of herbicide application influenced control. Mesotrione applied at 105 g ha À1 alone or combined with atrazine at 560 g ai ha À1 provided significantly greater waterhemp control (! 66%) when applied to small waterhemp plants (2 to 5 cm tall) compared with applications made to plants 5 to 10 or 10 to 15 cm tall. Glyphosate, glufosinate, fomesafen, lactofen, or acifluorfen provided greater waterhemp control (! 68%) 7 and 14 DAT than mesotrione, dicamba, or 2,4-D. Control of this population with atrazine, chlorimuron, and imazethapyr did not exceed 12%. Results of a greenhouse experiment with waterhemp plants grown from field-collected seed were similar to field data, and confirm the McLean County population was poorly controlled with HPPD, photosystem II, and acetolactate synthase inhibitors. Nomenclature: Acifluorfen; atrazine; chlorimuron; dicamba; fomesafen; glufosinate; glyphosate; imazethapyr; lactofen; mesotrione; tembotrione; topramezone; 2,4-D; waterhemp, Amaranthus tuberculatus (Moq.) Sauer var. rudis (Sauer) Costea and Tardif AMATU. Key words: Herbicide resistance, waterhemp management.Experimentos de campo e invernadero fueron realizados para caracterizar la respuesta de una población de Amaranthus tuberculatus proveniente del condado McLean en Illinois, a la aplicación foliar de herbicidas inhibidores de 4-hydroxyphenylpyruvate dioxygenase (HPPD) y determinar la sensibilidad de la población a herbicidas de grupos con otros sitios de acción. En el campo, plantas de A. tuberculatus de 10 a 15 cm de altura, tratadas con mesotrione a 105 g ai ha À1 , tembotrione a 92 g ai ha À1 , o topramezone a 18 g ai ha À1 , tuvieron una biomasa significativamente mayor (!10%) 14 d después del tratamiento (DAT) que A. tuberculatus cosechado el día de la aplicación del herbicida, indicando que hubo crecimiento después de la aplicación del herbicida. El estadio de desarrollo de A. tuberculatus al momento de la aplicación del herbicida influyó en el control. Mesotrione aplicado solo a 105 g ha À1 o combinado con atrazine a 560 g ai ha À1 brindó un control significativamente mayor (!66%) cuando se aplicó a plantas pequeñas de A. tuberculatus (2 a 5 cm de altura), al compararse con aplicaciones hechas a plantas de 5 a 10ó 10 a 15 cm de altura. Glyphosate, glufosinate, fomesafen, lactofen, o acifluorfen brindaron mayor control de A. tuberculatus (!68%) 7 y 14 DAT...

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“…Many weed species have been reported with enhanced metabolic resistance mediated by cytochrome P450s to various herbicide modes of action including ALS, acetyl Co‐A carboxylase (ACCase), photosystem II, and HPPD . Metabolic resistance in A. tuberculatus has been previously reported for ALS, photosystem II and HPPD herbicides, with different cytochrome P450 genes likely conferring HPPD resistance in different populations . 2,4‐D has been reported as an inducer of cytochrome P450 activity in plants both in vitro and in vivo , including the induction of demethylation and ring‐methyl hydroxylation of chlortoluron in tobacco ( Nicotiana tabacum ) cells .…”

Section: Discussionmentioning

confidence: 99%

Metabolism of 2,4‐dichlorophenoxyacetic acid contributes to resistance in a common waterhemp (Amaranthus tuberculatus) population

Figueiredo

Leibhart

Reicher

et al. 2018

Pest Management Science

109

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Figueiredo, Marcelo R. A.; Leibhart, Lacy J.; Reicher, Zachary J.; Tranel, Patrick J.; Nissen, Scott J.; Westra, Philip; Bernards, Mark L.; Kruger, Greg R.; Gaines, Todd A.; and Jugulam, Mithila, "Metabolism of 2,4-dichlorophenoxyacetic acid contributes to resistance in a common waterhemp (Amaranthus tuberculatus) population" (2017 This article has been accepted for publication and undergone full peer review but has not been through the copyediting, typesetting, pagination and proofreading process, which may lead to differences between this version and the Version of Record. Please cite this article as doi: 10.1002/ps.

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Nontarget-Site Resistance to ALS Inhibitors in Waterhemp (Amaranthus tuberculatus)

Cited by 50 publications

References 25 publications

Independent Evolution of Acetolactate Synthase–inhibiting Herbicide Resistance in WeedySorghumPopulations across Common Geographic Regions

Independent Evolution of Acetolactate Synthase–inhibiting Herbicide Resistance in WeedySorghumPopulations across Common Geographic Regions

Responses of a Waterhemp (Amaranthus tuberculatus) Population Resistant to HPPD-Inhibiting Herbicides to Foliar-Applied Herbicides

Metabolism of 2,4‐dichlorophenoxyacetic acid contributes to resistance in a common waterhemp (Amaranthus tuberculatus) population

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