Nonsurgical Periodontal Therapy Combined with Laser and Photodynamic Therapies for Periodontal Disease in Immunosuppressed Rats

Bottura, P.E.; Milanezi, J.; Fernandes, Leandro Araújo; Caldas, Heloisa Cristina; Abbud-Filho, Mário; Garcia, Valdir Gouveia; Baptista, Maria Alice Sperto Ferreira

doi:10.1016/j.transproceed.2011.03.083

Cited by 16 publications

(10 citation statements)

References 52 publications

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“…Figure 1 shows the experimental study model. The minimum sample size of animals was determined by power analysis for sample size calculation based on the data with similar study design given by Bottura, et al 15 (2011). As per the result of the power analysis performed by using the Power and Sample Size program, the estimated average for the bone loss parameter was determined as 0,4; the standard deviation as 0,2; the power when received was identified as 0,80, whereas the number of samplings determined for α:0,05 was identified as n:5 at miminum for each time period.…”

Section: Methodsmentioning

confidence: 99%

Effects of diode laser application on inflammation and mpo in periodontal tissues in a rat model

et al. 2018

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Objective In this study, we aimed to histologically and immunologically evaluate the effect of diode laser treatment when applied adjunctive to scaling and root planing (SRP) in an experimental periodontitis model.Materials and methods We used Wistar-Albino rats (n=60) with average weight of 230 g. Experimental periodontitis was induced by ligature at the right and left first mandibular molar teeth in all rats. After 11 days, the ligature was removed and rats were divided into two groups. The control group (n=30) received only SRP treatment, while the laser group (n=30) received a diode laser (GaAlAs, 810 nm, 1 W, 10 J, 20 s) treatment adjunctive to SRP. Ten rats in each group were sacrificed after 7, 15, and 30 days. Histopathological examination was performed in the left mandible of rats. Myeloperoxidase (MPO) was evaluated by western blot in the gingival specimens from the right mandible.Results MPO levels in the laser group were statistically significantly lower compared with the control group (p≤0.05). There was no statistically significance at any time between MPO levels in the control group (p>0.05). MPO levels in the laser group at the 7th day were statistically significantly higher compared to the 15th (p≤0.05) and the 30th day (p≤0.05). Inflammatory cell infiltration decreased over time in both groups and was statistically significantly lower in the laser group than in the control group at all times (p≤0.01).Conclusions Within the limits of this study, we suggest that diode laser application is an adjunctive treatment because it reduced inflammation and MPO when applied in addition to SRP. On the other hand, more studies are needed for the assessment of the effects of diode laser application to periodontal tissues.

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Section: Methodsmentioning

confidence: 99%

Effects of diode laser application on inflammation and mpo in periodontal tissues in a rat model

et al. 2018

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“…Bottura et al . used a model of immunosuppressed rats to demonstrate the effect of PDT on reducing bone loss resulted in experimental periodontitis [76]. Several more studies were in agreement with the previous literature, and further manifested the effectiveness of PDT in treatment of periodontal disease [77–79].…”

Section: Photodynamic Therapy As An Anti-infectivementioning

confidence: 57%

Light based anti-infectives: ultraviolet C irradiation, photodynamic therapy, blue light, and beyond

Yin

Dai

Avci

et al. 2013

Current Opinion in Pharmacology

229

172

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Owing to the worldwide increase in antibiotic resistance, researchers are investigating alternative anti-infective strategies to which it is supposed microorganisms will be unable to develop resistance. Prominent among these strategies, is a group of approaches which rely on light to deliver the killing blow. As is well known, ultraviolet light, particularly UVC (200–280nm), is germicidal, but it has not been much developed as an anti-infective approach until recently, when it was realized that the possible adverse effects to host tissue were relatively minor compared to its high activity in killing pathogens. Photodynamic therapy is the combination of non-toxic photosensitizing dyes with harmless visible light that together produce abundant destructive reactive oxygen species (ROS). Certain cationic dyes or photosensitizers have good specificity for binding to microbial cells while sparing host mammalian cells and can be used for treating many localized infections, both superficial and even deep-seated by using fiber optic delivered light. Many microbial cells are highly sensitive to killing by blue light (400–470 nm) due to accumulation of naturally occurring photosensitizers such as porphyrins and flavins. Near infrared light has also been shown to have antimicrobial effects against certain species. Clinical applications of these technologies include skin, dental, wound, stomach, nasal, toenail and other infections which are amenable to effective light delivery.

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“…aPDT counteracts the impaired healing in diabetic and/or immunosuppressed animals [ 102 , 103 , 104 ]. Additionally, aPDT has been shown to reduce bone loss and promote the repair of bone tissue, which is modulated by immunosuppressive drugs [ 105 , 106 , 107 , 108 ]. The influence of aPDT on periodontal bone loss related to diabetes in rats was initially reported by Almeida et al [ 67 ].…”

Section: Effect Of Apdt On Periodontitis With An Unusual Host Respmentioning

confidence: 99%

Adjunctive Application of Antimicrobial Photodynamic Therapy in Nonsurgical Periodontal Treatment: A Review of Literature

Kikuchi

Mogi

Okabe

et al. 2015

IJMS

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Periodontal disease is caused by dental plaque biofilms, and the removal of these biofilms from the root surface of teeth plays a central part in its treatment. The conventional treatment for periodontal disease fails to remove periodontal infection in a subset of cases, such as those with complicated root morphology. Adjunctive antimicrobial photodynamic therapy (aPDT) has been proposed as an additional treatment for this infectious disease. Many periodontal pathogenic bacteria are susceptible to low-power lasers in the presence of dyes, such as methylene blue, toluidine blue O, malachite green, and indocyanine green. aPDT uses these light-activated photosensitizer that is incorporated selectively by bacteria and absorbs a low-power laser/light with an appropriate wavelength to induce singlet oxygen and free radicals, which are toxic to bacteria. While this technique has been evaluated by many clinical studies, some systematic reviews and meta-analyses have reported controversial results about the benefits of aPDT for periodontal treatment. In the light of these previous reports, the aim of this review is to provide comprehensive information about aPDT and help extend knowledge of advanced laser therapy.

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Nonsurgical Periodontal Therapy Combined with Laser and Photodynamic Therapies for Periodontal Disease in Immunosuppressed Rats

Cited by 16 publications

References 52 publications

Effects of diode laser application on inflammation and mpo in periodontal tissues in a rat model

Effects of diode laser application on inflammation and mpo in periodontal tissues in a rat model

Light based anti-infectives: ultraviolet C irradiation, photodynamic therapy, blue light, and beyond

Adjunctive Application of Antimicrobial Photodynamic Therapy in Nonsurgical Periodontal Treatment: A Review of Literature

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