Nonspecific Symbiosis Between <i>Sophora flavescens</i> and Different Rhizobia

Liu, Yuan Hui; Jiao, Yin; Liu, Li Xue; Wang, Dan; Tian, Chang; Wang, En Tao; Wang, Lei; Chen, Wen Xin; Wu, Shang Ying; Guo, Baoyuan; Guan, Zha Gen; Poinsot, Véréna

doi:10.1094/mpmi-05-17-0117-r

Cited by 15 publications

(17 citation statements)

References 66 publications

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“…Bradyrhizobiaceae were depleted in roots of soybeans inoculated with the noeI mutant. This might be explained by a reduction in compatibility between the host plant and Bradyrhizobium caused by noeI mutation [50].…”

Section: Discussionmentioning

confidence: 99%

“…1a) that consisted of two rhizobial genotype treatments and two planting patterns. The rhizobial genotype treatments included: 1) B. diazoe ciens USDA 110 wild type, isolated from soybean [52]; and 2) B. diazoe ciens USDA 110 noeI mutant, obtained from our previous study [50]. The two planting patterns were 1) planted with cultivated soybean (C08) and 2) intact soil without plants (unplanted).…”

Section: Greenhouse Experiments and Symbiotic Phenotype Testingmentioning

confidence: 99%

“…NGR234 nodulating several host plants [48,49]. However, a recent study conducted on B. diazoe ciens USDA 110 found that noeI has a vital role in maintaining nitrogen xation e ciency in soybean [50]. While nodulation phenotypes and host nitrogen status are known to have an impact on the structure of root-and shoot-associated microbiomes in soybean [42,51], the effect of genetic variation in nitrogen-xing symbionts is unknown.…”

Section: Introductionmentioning

confidence: 99%

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The symbiotic capacity of rhizobium shapes root-associated microbiomes

Liu¹,

Ma²,

Chen³

et al. 2020

Preprint

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Background: Root-microbiome interactions are of central importance for plant performance and yield. A distinctive feature of legumes in this context is that they engage in symbiosis with rhizobia, which are abundant in soils and include both symbiotic and non-symbiotic bacterial strains. If and how the capacity of rhizobia to form symbiosis modulates root-associated microbiomes are not well understood. Results: We address this question by inoculating soybean (Glycine max) plants with wild type (WT) or a noeI mutant of Bradyrhizobium diazoefficiens. The noeI mutant produces a defective Nod factor and is thus compromised in its ability to establish functional symbiosis. Compared to soybean plants inoculated with WT rhizobia, plants inoculated with the noeI mutant showed a significant decrease in nodulation and root-flavonoid exudation, and exhibited strong changes in microbiome assembly in the rhizosphere and the rhizoplane. NoeI mutant-inoculated roots exhibited reduced diversity, co-occurrence interactions and a substantial depletion of beneficial microbes on the roots. The effects of the noeI mutation were absent in soils without plants, demonstrating that they are plant dependent. Complementation experiments showed that flavonoid supplementation is sufficient to restore recruitment of beneficial microbes. Conclusion: The results illustrate that the capacity of a rhizobium to form microbial symbiosis dramatically alters root-associated microbiomes, most likely by changing root exudation patterns. The results of this study have important implications for our understanding of the evolution of plant-microbiome interactions in the context of plant-bacterial symbioses.

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Section: Discussionmentioning

confidence: 99%

Section: Greenhouse Experiments and Symbiotic Phenotype Testingmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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The symbiotic capacity of rhizobium shapes root-associated microbiomes

Liu¹,

Ma²,

Chen³

et al. 2020

Preprint

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“…A previous study found that the growth of SFA is greatly influenced by rhizobial nodulation using different rhizobial strains and specific gene mutants (Liu et al . 2018a). Plants grow poorly (characterized by the yellow leaves and lower chlorophyll content) when they are inoculated with a rhizobium harbouring a nodC gene mutation or when they are not inoculated (Liu et al .…”

Section: Resultsmentioning

confidence: 99%

Bradyrhizobium arachidis mediated enhancement of (oxy)matrine content in the medicinal legume Sophora flavescens

Meng

Jiao

et al. 2021

Lett Appl Microbiol

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Effect of rhizobial inoculation and nitrate application on the content of bioactive compounds in legume plants is an interesting aspect for interactions among microbes, plants and chemical fertilizers, as well as for cultivated practice of legumes. In this study, nitrate (0, 5 and 20 mmol l−1) and Bradyrhizobium arachidis strain CCBAU 051107T were applied, individually or in combination, to the root rhizosphere of the medicinal legume Sophora flavescens Aiton (SFA). Then the plant growth, nodulation and active ingredients including (oxy)matrine of SFA were determined and compared. Rhizobial inoculation alone significantly increased the numbers and fresh weight of root nodules. Nodulation was significantly inhibited due to nitrate (5 and 20 mmol l−1). Only oxymatrine was detected in the control plants without rhizobial inoculation and nitrate supplement, while both oxymatrine and matrine were synthesized in plants treated with inoculation of B. arachidis or supplied with nitrate. The content of oxymatrine was the highest in plants inoculated solely with rhizobia and was not significantly altered by additional application of nitrate. Combinations of B. arachidis inoculation and different concentrations of nitrate did not significantly change the concentrations of (oxy)matrine in the plant. In conclusion, sole rhizobial inoculation was the best approach to increase the contents of key active ingredients oxymatrine and matrine in the medicinal legume SFA.

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“…The nitrogen fixing ability of B. diazoefficiens USDA110 noeI mutant was degraded. Furthermore, single noeI or double nodZnoeI mutant of S. fredii CCBAU45436 showed decreasing nodule number, although their nitrogen fixing abilities were normal (Liu et al, 2017b ). Although there are no specific genes shared by the soybean microsymbionts (Tian et al, 2012 ), all above indicates that rhizobial Nod factor compositions are much conserved, and that they are more related to host range than to phylogenetic relationship.…”

Section: Discussionmentioning

confidence: 99%

Conserved Composition of Nod Factors and Exopolysaccharides Produced by Different Phylogenetic Lineage Sinorhizobium Strains Nodulating Soybean

et al. 2018

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The structural variation of symbiotic signals released by rhizobia determines the specificity of their interaction with legume plants. Previous studies showed that Sinorhizobium strains from different phylogenetic lineages had different symbiotic performance on certain cultivated soybeans. Whether they released similar or different symbiotic signals remained unclear. In this study, we compared their nod and exo gene clusters and made a detailed structural analysis of Nod factors and EPS by ESI-MS/MS and two dimensions NMR. Even if there are some differences among nod or exo gene clusters; they produced much conserved Nod factor and EPS compositions. The Nod factors consist of a cocktail of β-(1, 4)-linked tri-, tetra-, and pentamers of N-acetyl-D-glucosamine (GlcNAc). The C2 position on the non-reducing terminal end is modified by a lipid chain that contains 16 or 18 atoms of carbon–with or without unsaturations-, and the C6 position on the reducing residue is decorated by a fucose or a 2-O-methylfucose. Their EPS are composed of glucose, galactose, glucuronic acid, pyruvic acid in the ratios 5:1:2:1 or 6:1:2:1. These findings indicate that soybean cultivar compatibility of Sinorhizobium strains does not result from Nod factor or EPS structure variations. The structure comparison of the soybean microbionts with other Sinorhizobium strains showed that Nod factor structures of soybean microbionts are much conserved, although there are no specific genes shared by the soybean microsymbionts. EPS produced by Sinorhizobium strains are different from those of Bradyrhizobium. All above is consistent with the previous deduction that Nod factor structures are related to host range, while those of EPS are connected with phylogeny.

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Nonspecific Symbiosis Between Sophora flavescens and Different Rhizobia

Cited by 15 publications

References 66 publications

The symbiotic capacity of rhizobium shapes root-associated microbiomes

The symbiotic capacity of rhizobium shapes root-associated microbiomes

Bradyrhizobium arachidis mediated enhancement of (oxy)matrine content in the medicinal legume Sophora flavescens

Conserved Composition of Nod Factors and Exopolysaccharides Produced by Different Phylogenetic Lineage Sinorhizobium Strains Nodulating Soybean

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