Nonspecific inhibitor released by T acceptor cells reduces the production of interleukin-2

Malkovsky, Miroslav; Asherson, G. L.; Stockinger, Brigitta; Watkins, M C

doi:10.1038/300652a0

Cited by 56 publications

(16 citation statements)

References 23 publications

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“…The specific IL-2 activity after purification was -6 x 106 units per mg of protein. The units of IL-2 activity were determined in a standard bioassay (27,28) One hundred microliters of such a solution of IL-2 (1200 units) was injected intraperitoneally into tolerant mice 58 and 59 days after transplantation. As a control for stress, some tolerant mice received intraperitoneal injections of 100 ,ul of balanced salt solution containing human albumin (1 mg-ml-') at the same time.…”

Section: Methodsmentioning

confidence: 99%

Acquired immunological tolerance of foreign cells is impaired by recombinant interleukin 2 or vitamin A acetate.

Malkovský¹,

Medawar²,

Thatcher³

et al. 1985

Proc. Natl. Acad. Sci. U.S.A.

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The susceptibility of newborn mice to the inception of tolerance after exposure to antigen is associated with their deficiency in the production of endogenous interleukin 2 (IL-2). As further evidence of the complicity of IL-2 in the inception and maintenance of tolerance, it is shown here that a solid and long-lasting state of tolerance induced by the intravenous injection into newborn CBA mice of lymphoid cells from (CBA x C57BL/1OScSn)Fj hybrids can be brought to an end by the administration of exogenous IL-2 or by supplementing an otherwise normal diet with vitamin A acetate, the effect of which is to increase the proportion of the moiety of the T-cell population that produces IL-2. These results indicate that certain nonspecific stimuli can influence whether immunological tolerance is maintained.

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Section: Methodsmentioning

confidence: 99%

Acquired immunological tolerance of foreign cells is impaired by recombinant interleukin 2 or vitamin A acetate.

Malkovský¹,

Medawar²,

Thatcher³

et al. 1985

Proc. Natl. Acad. Sci. U.S.A.

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“…Plates were incubated at 378C and were pulsed (1 Ci/well) with tritiated thymidine (Du Pont) on day 2 or day 4 (as indicated), and harvested 24 h later. Tritiated thymidine incorporation was measured by liquid scintillation counting [40]. The mean ct/min of triplicate samples was used to calculate the SI as follows: SI (responder with stimulator cells ct/min -stimulator cells alone ct/min)/ responder cells alone ct/min.…”

Section: Proliferation Assaysmentioning

confidence: 99%

γδ T lymphocyte responses to HIV

Wallace¹,

Bartz²,

Chang³

et al. 1996

Clinical and Experimental Immunology

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“…Cultures were incubated for 72 hr and for an additional 18 hr with 0.5 ,uCi of [methyl-3H]thymidine (1 Ci = 37 GBq) per well. Cells were harvested and the amount of radioactivity incorporated was determined by liquid scintillation counting (22). Supernatants from the PBMC cultures stimulated with PHA in the presence or absence of drugs were tested for IL-2 content by assay on CTLL-2 cells (23,24).…”

mentioning

confidence: 99%

Inhibition of human immunodeficiency virus replication by nonimmunosuppressive analogs of cyclosporin A.

Bartz

Hohenwalter²,

Hu³

et al. 1995

Proc. Natl. Acad. Sci. U.S.A.

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Analogs of the immunosuppressive cyclic undecapeptide cyclosporin A (CsA) with substitutions in positions 1, 4, 6, and/or 11 were rationally designed to possess substantially diminished or no immunosuppressive activity. When these compounds were assayed for their capacity to interfere with the replication of human immunodeficiency virus, some displayed a potent antiviral activity in newly infected cells. However, only CsA could interfere with virus replication in persistently infected cells. One CsA analog with antiviral activity costimulated the phytohemagglutinin-induced production of interleukin 2 by human lymphocytes. Human immunodeficiency virus particles from drug-exposed cells showed lower infectivity than virions from untreated cells. Thus, these nonimmunosuppressive analogs of CsA constitute a promising class of lead compounds to develop drugs for effective treatment of the acquired immunodeficiency syndrome.

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Nonspecific inhibitor released by T acceptor cells reduces the production of interleukin-2

Cited by 56 publications

References 23 publications

Acquired immunological tolerance of foreign cells is impaired by recombinant interleukin 2 or vitamin A acetate.

Acquired immunological tolerance of foreign cells is impaired by recombinant interleukin 2 or vitamin A acetate.

γδ T lymphocyte responses to HIV

Inhibition of human immunodeficiency virus replication by nonimmunosuppressive analogs of cyclosporin A.

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