Nonspecific cytotoxic cells as effectors of immunity in fish

Evans, Donald L.; Jaso-Friedmann, Liliana

doi:10.1016/0959-8030(92)90059-7

Cited by 118 publications

(55 citation statements)

References 56 publications

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“…Cell-mediated cyto toxic activity, in particular nonspecific cell-mediated cytotoxic activity has been described in fish (Evans and Jaso-Friedmann, 1992;Yoshinaga et al, 1994;Kurata et al, 1995). On the other hand, to our knowledge, spe cific-cell mediated cytotoxicity in fish have been studied only in xenogeneic responses (Sakai, 1984), allogeneic responses (Hashimoto and Ikeda, 1987;Stuge et al, 1997;Hasegawa et al, 1998;Fischer et al, 1998;Nakanishi and Ototake 1999), and in vitro autologous responses (Verlhac et al, 1990).…”

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confidence: 99%

In vivo Priming of TNP-reactive Effector Cells in Carp.

et al. 1999

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ABSTRACT-In a study of immune mechanisms in fish, specific cell-mediated cytotoxic activity against trinitrophenyl (TNP)-modified autologous peripheral blood leucocytes (PBL) was success fully induced in in vivo primed carp Cyprinus carpio. The cytotoxic activity increased in proportion to the number of immunizations. The cytotoxic activity was shown to be gradually reduced with time after in vivo sensitization, and was finally lost. In addition, cytotoxic activities against TNPmodified allogeneic PBLs (T-ALLO-PBL) in carp immunized with TNP-modified autologous PBLs (T-AUTO-PBL) were not detected and effector cells from carp immunized with T-ALLO-PBL were not significantly cytotoxic against T-AUTO-PBL. Thus, the occurrence of a genetic restriction in carp was shown using the in vivo priming model. These results suggest that specific cell-mediated cytotoxicity of in vivo primed carp in some respects resembles mammalian cytotoxic T cells.

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In vivo Priming of TNP-reactive Effector Cells in Carp.

et al. 1999

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“…Serological and biochemical studies revealed the presence of class I MHC Ags in erythrocytes of different vertebrate species including fish (11,12). The most extensively studied cytotoxic cells in teleosts are the nonspecific cytotoxic cells, which are able to spontaneously kill a variety of targets (13)(14)(15). The inheritance of phagocytosis and lysozyme activity levels in tilapia was reported by Sarder et al (16).…”

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confidence: 91%

Altered Self-Erythrocyte Recognition and Destruction in an Inbred Line of Tilapia (Oreochromis aureus)

Shirak

Bendersky

Hulata

et al. 2006

The Journal of Immunology

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Carboxyfluorescein diacetate (cFDA)-stained autologous and syngeneic tilapia (Oreochromis aureus) erythrocytes are recognized by effector peripheral blood leukocytes and lysed after a short culture period of 4 h. The hemolysis level was evaluated by measuring the fluorescence of the released cFDA. The degree of lysis of stained target erythrocytes of 60 individuals revealed a trimodal distribution statistically stratified into three groups of low (LR), intermediate (IR), and high (HR) responders. Depletion of the majority of phagocytes from leukocytes lowered the lysis level of HR to that of LR. A highly significant increase of LR cytotoxicity was obtained after the addition of conditioned medium from HR but only in the presence of phagocytes. Genetic analysis of offspring from four crosses (IR × HR, IR × LR, HR × LR, and LR × LR) revealed a quantitative trait locus (QTL) segregating for the level of response linked to markers UNH207 and UNH231 on linkage group 6 of tilapia. Based on segregation analysis of 58 gynogenetic BIU-1 offspring, the distances from the centromere were estimated as 21.5, 11.5, and 9.0 cM for UNH207, UNH231, and the QTL, respectively. It is suggested that 1) self-target recognition and destruction requires both cFDA-altered self-erythrocyte membrane and membrane structures normally present in autologous, syngeneic, and xenogeneic targets; 2) natural cytotoxic cells and/or macrophages are involved in erythrocyte lysis; and 3) the lysis level is codominantly inherited by a QTL segregating on tilapia linkage group 6.

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“…2). However, these effectors, suggested to be homologues of the NK cells of "higher" animals, have not been well characterized at the molecular level.…”

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Development and Analysis of Various Clonal Alloantigen- Dependent Cytotoxic Cell Lines from Channel Catfish

Stuge

Wilson

Zhou

et al. 2000

The Journal of Immunology

111

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To determine the phenotypes of cytotoxic cells in channel catfish, clonal alloantigen-dependent leukocyte lines were established from mixed leukocyte cultures. Each clone was analyzed for expression of TCR α and β genes by RT-PCR and for target cell specificity by 51Cr-release assay. Based on the above criteria, the following five different cell types were identified among the 19 clones analyzed: 1) TCR αβ+ allospecific cytotoxic cells, 2) TCR αβ+ nonspecific cytotoxic cells, 3) allospecific TCR αβ+ noncytotoxic cells, 4) TCR αβ− nonspecific cytotoxic cells, and 5) TCR αβ− allospecific cytotoxic cells. The demonstration of cloned, TCR αβ+, allospecific cytotoxic effectors provides the strongest evidence to date for the existence of cytotoxic T cells in fish.

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Nonspecific cytotoxic cells as effectors of immunity in fish

Cited by 118 publications

References 56 publications

In vivo Priming of TNP-reactive Effector Cells in Carp.

In vivo Priming of TNP-reactive Effector Cells in Carp.

Altered Self-Erythrocyte Recognition and Destruction in an Inbred Line of Tilapia (Oreochromis aureus)

Development and Analysis of Various Clonal Alloantigen- Dependent Cytotoxic Cell Lines from Channel Catfish

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