Nonspecific and Thiol-Specific Binding of DNA to Gold Nanoparticles

Sandström, Pär; Boncheva, Mila; Åkerman, Björn

doi:10.1021/la034348u

Cited by 158 publications

(160 citation statements)

References 21 publications

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“…22,23 The generally accepted adsorption model for this process is shown in Figure 1B, where DNA wraps around the AuNP. 22,[24][25][26] The adsorption of non-thiolated DNA has also found important applications in analytical chemistry, [27][28][29][30][31][32][33][34] polymerase chain reactions, 35,36 nanoparticle synthesis, 37,38 and medicine. 37,39 We recently reported that at neutral pH, each 13 nm AuNP adsorbs just around twenty 12-mer DNAs even after overnight incubation, 40 which is consistent with the results from the literature using polyadenosine (e.g A20) as an anchoring block for DNA adsorption with the salt aging approach.…”

Section: This Document Is the Accepted Manuscript Version Of A Publismentioning

confidence: 99%

Instantaneous Attachment of an Ultrahigh Density of Nonthiolated DNA to Gold Nanoparticles and Its Applications

et al. 2012

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The last 16 years have witnessed the landmark development of polyvalent thiolated DNA functionalized gold nanoparticles (AuNPs) possessing striking properties within the emerging field of nanobiotechnology. Many novel properties of this hybrid nanomaterial are attributed to the dense DNA shell. However, the question of whether non-thiolated polyvalent DNA-AuNP could be fabricated with high DNA density and similar properties as its thiolated counterpart has not been explored in detail.Herein, we report that by simply tuning the pH of the DNA/AuNP mixture, an ultrahigh capacity of nonthiolated DNA can be conjugated to AuNPs in a few minutes, resulting in polyvalent DNA-AuNP conjugates with cooperative melting behavior, a typical property for polyvalent thiolated DNA functionalized AuNPs. With this method, large AuNPs (e.g., 50 nm) can be functionalized to achieve colorimetric detection of sub-nM DNA. Further, this fast and stable DNA loading was employed to separate AuNPs of different size. We propose that a large fraction of the attached DNAs are adsorbed via one or a few terminal bases to afford the high loading capacity and the ability to hybridize with the complementary DNA. This discovery not only offers a time-and cost-effective way to functionalize AuNPs with a high density of non-thiolated DNA, but also provides new insights into the fundamental understanding of how DNA strands with different sequences interact with AuNPs.

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Section: This Document Is the Accepted Manuscript Version Of A Publismentioning

confidence: 99%

Instantaneous Attachment of an Ultrahigh Density of Nonthiolated DNA to Gold Nanoparticles and Its Applications

et al. 2012

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“…The prediction was also confirmed by comparing the results of gel electrophoresis. Furthermore, gel electrophoresis has been previously utilised in nanotechnology to investigate the biding behaviour of L-lysine [24], to separate DNA-capped nanogold [25,26] and protein-capped GNPs [27].…”

Section: Introductionmentioning

confidence: 99%

Fabrication of capped gold nanoparticles by using various amino acids

Zare

Khoshnevisan

Barkhi

et al. 2013

Journal of Experimental Nanoscience

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The production of gold nanoparticles (GNPs) by amino acid is one of the most attractive and interesting subjects in nanobiotechnology. In this study, amino acids have been utilised as a reducing agent and also an agent for capping GNPs. The GNPs were prepared using a reduction solution containing gold cations with optimum concentration of gold salt (5 mM), and also functionalised by glutamic acid, phenylalanine and tryptophan with optimum concentration of amino acids (25 mM). The optimum condition of gold solution and amino acids were achieved by ultraviolet-visible spectroscopy. The size of nanoparticles was obtained 5-20, 10-20 and 20-30 nm, respectively, by transmission electron microscopy and dynamic light scattering techniques. The results obtained from experimental and quantum calculations confirm that amino acids have strong bond while they have anion binding. Moreover, the free carboxylic groups of capped GNPs are one of the suitable and capable beads for binding biological agents. As a result, the medical applications of amino acids and proteins can be used as a practical method due to the strong interaction of peripheral amine groups with nanoparticles.

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“…Following the same theme, the coating of citrate-stabilized AuNPs with bovine serum albumin (BSA) through electrostatic interactions has been demonstrated [38]. Non-specific binding has also been shown between single strand DNA and the surface of colloidal gold particles [31,32].…”

Section: Introductionmentioning

confidence: 91%

“…'Grafting-to' [22,[29][30][31][32][33][34][35][36][37][38] is now the accepted nomenclature for post modification of the surface of a colloidal dispersion using pre-synthesized polymers. Boyer et al [29] showed how a chemical affinity could result in the directed adsorption of functional end groups (thiol and trithiocarbonate) of block copolymers constructed from PEG analogues onto the surface of citrate-stabilized gold nanoparticles (AuNPs).…”

Section: Introductionmentioning

confidence: 99%

“…The former reportedly requires an incubation time of 72 hours, while a week long duration is required of the latter. Thiol linkers have also been shown to covalently bind DNA onto gold particles [31,32]. Graftingto has also been demonstrated with the introduction of CdS quantum dots to an aqueous solution containing thiolated pNIPAM chains [33].…”

Section: Introductionmentioning

confidence: 99%

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Facile synthesis of gold core–polymer shell responsive particles

D’Souza-Mathew¹,

Cayre²,

Hunter³

et al. 2013

Journal of Colloid and Interface Science

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The free adsorption of an end-functionalised weak polybase, poly dimethylaminoethyl methacrylate (pDMAEMA), on the surface of colloidal gold nanoparticles (AuNPs) as a route to produce a responsive core-shell nanoparticle is explored here. Optimal conditions for the physisorption of the polymeric chains onto the colloidal nanoparticles are explored. A dense coverage is facilitated by rapidly mixing the well solvated pH responsive homopolymer, at low pH, into a relatively poor solvent environment, at higher pH, containing a stable dispersion of charge-stabilized gold nanoparticles. The rapid pH change causes the polymer chains to concurrently collapse and adsorb onto the gold nanoparticles. In order to achieve sterically stable, monodisperse and responsive core shell nanoparticles, a crucial factor is the pH difference of the systems prior to their mixing. Once adsorbed, end-functional thiol groups on the adsorbed polymer chains can form more permanent covalent attachments with the core particles. Dynamic light scattering coupled with mobility data of pH titration experiments show that the core-shell particles exhibit a responsive character consistent with the observed potentiometric titration data of the polymer.The same particles demonstrate reversible aggregation when cycled between pH extremes. This is confirmed by shifts in the SPR peak of the corresponding UV-Vis absorption profile. The ease and flexibility of this strategy for core-shell particle production, coupled with the stability and responsiveness of the product, make this a promising colloidal coating mechanism.

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Nonspecific and Thiol-Specific Binding of DNA to Gold Nanoparticles

Cited by 158 publications

References 21 publications

Instantaneous Attachment of an Ultrahigh Density of Nonthiolated DNA to Gold Nanoparticles and Its Applications

Instantaneous Attachment of an Ultrahigh Density of Nonthiolated DNA to Gold Nanoparticles and Its Applications

Fabrication of capped gold nanoparticles by using various amino acids

Facile synthesis of gold core–polymer shell responsive particles

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