Nonselective Endothelin-Receptor Antagonism Attenuates Hemodynamic Changes After Massive Pulmonary Air Embolism in Dogs

Serum thromboxane B2 (TXB2) Determination is Influenced by Sample Incubation Temperature in Healthy Beagle Dogs

“…While mean TXB 2 concentration in samples incubated at 37 C for one hour was similar to the values of TXB 2 concentration in studies that incubated samples at the same conditions, mean serum TXB 2 concentration in samples incubated at room temperature (550 ± 257 g/l) was not in accordance with the results of other research groups that used canine serum or plasma samples without incubation at 37 C and reported TXB 2 concentrations of 1.5 g/l or less (Hashimoto et al 2002;Sunose et al 2001a;Sunose et al 2001b;Tanus-Santos et al 2000;Yamanaka et al 1993). In contrast to extremely low TXB 2 concentrations in plasma, an exceptionally high amount of TXB 2 is produced ex vivo in blood after clotting under controlled conditions that is incubation for one hour at 37 C (Brideau et al 1996;Patrignani et al 1997).…”

“…In some studies (Blain et al 2002;Gilmer et al 2002;Jones et al 2002;McKellar et al 1990;Rinder et al 2002;Streppa et al 2002) blood samples for serum TXB 2 determination were incubated during blood clotting at 37 C for one hour as recommended (Brideau et al 1996;Patrignani et al 1997), whereas other studies (Frelinger et al 2006;Hashimoto et al 2002;Morchon et al 2006;Sunose et el. 2001a;Sunose et al 2001b;Tanus-Santos et al 2000) did not mention the incubation of samples. Instead of serum, plasma was used for TXB 2 determination in a few studies (Jacoby et al 2000;Knijff-Dutmer et al 2002;Yamanaka et al 1993).…”

Pulmonary embolism and cardiac enzymes

“…12 An increase in pulmonary artery pressure and pulmonary vascular resistance after PE is the most likely cause of the leak of cardiac enzymes from RV myocytes. An increase in pulmonary artery pressure is secondary to 3 main mechanisms: 13,14 (1) mechanical obstruction of RV and pulmonary artery, (2) arterial neurogenic constriction, and (3) release of humoral mediators.…”

Infusão intravenosa de vasopressina causa efeitos cardiovasculares adversos dose-dependentes em cães anestesiados

“…Animal handling and experimental model preparation: the animals were prepared as described by Tanus-Santos 21 . After a fasting night with free access to water, the animals were anesthetized with a loading dose of sodium pentobarbital (10 mg.kg -1 , IV) and an adequate level of anesthesia was maintained by a continuously IV infusion of the same drug (2-4 mg.kg -1 .h -1 ).…”