Nonproliferating Cells of Tumors

Gb, Pierce

doi:10.1159/000397542

Cited by 2 publications

(2 citation statements)

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“…Studies by G Barry Pierce showed that only the undifferentiated cells in these tumors give rise to teratomas, the differentiated cells do not [6], [7], [9]. Utilizing these undifferentiated cells allows evaluation of the mammary microenvironment's ability to reprogram embryonic cells that have not yet committed to a cell fate, and test the mammary gland's capacity to alter the teratoma-forming capability of mouse ES cells.…”

Section: Introductionmentioning

confidence: 99%

Embryonic Stem Cells Are Redirected to Non-Tumorigenic Epithelial Cell Fate by Interaction with the Mammary Microenvironment

et al. 2013

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Experiments were conducted to redirect mouse Embryonic Stem (ES) cells from a tumorigenic phenotype to a normal mammary epithelial phenotype in vivo. Mixing LacZ-labeled ES cells with normal mouse mammary epithelial cells at ratios of 1∶5 and 1∶50 in phosphate buffered saline and immediately inoculating them into epithelium-divested mammary fat pads of immune-compromised mice accomplished this. Our results indicate that tumorigenesis occurs only when normal mammary ductal growth is not achieved in the inoculated fat pads. When normal mammary gland growth occurs, we find ES cells (LacZ+) progeny interspersed with normal mammary cell progeny in the mammary epithelial structures. We demonstrate that these progeny, marked by LacZ expression, differentiate into multiple epithelial subtypes including steroid receptor positive luminal cells and myoepithelial cells indicating that the ES cells are capable of epithelial multipotency in this context but do not form teratomas. In addition, in secondary transplants, ES cell progeny proliferate, contribute apparently normal mammary progeny, maintain their multipotency and do not produce teratomas.

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Section: Introductionmentioning

confidence: 99%

Embryonic Stem Cells Are Redirected to Non-Tumorigenic Epithelial Cell Fate by Interaction with the Mammary Microenvironment

et al. 2013

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“…Following in their footsteps, the University of Colorado pathologist, Barry Pierce, identified mammary stem cells in mouse mammary tumors (Pierce and Verney 1961;Pierce 1974Pierce , 1975Pierce , 1977Pierce , 1983Pierce et al 1977;Sell and Pierce 1994). Pierce began his stem cell research in the late 1950s with Frank Dixon (University of Pittsburgh) with the help of Roy Stevens of the Jackson Laboratory, a colleague of C.C.…”

Section: Serial Transplantation and The Concept Of Tumor Recapitulationmentioning

confidence: 99%

A Compendium of the Mouse Mammary Tumor Biologist: From the Initial Observations in the House Mouse to the Development of Genetically Engineered Mice

Cardiff

Kenney

2010

Cold Spring Harbor Perspectives in Biology

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For over a century, mouse mammary tumor biology and the associated mouse mammary tumor virus (MMTV) have served as the foundation for experimental cancer research, in general, and, in particular, experimental breast cancer research. Spontaneous mouse mammary tumors were the basis for studies of the natural history of neoplasia, oncogenic viruses, host responses, endocrinology and neoplastic progression. However, lacking formal proof of a human mammary tumor virus, the preeminence of the mouse model faded in the 1980s. Since the late 1980s, genetically engineered mice (GEM) have proven extremely useful for studying breast cancer and have become the animal model for human breast cancer. Hundreds of mouse models of human breast cancer have been developed since the first demonstration in 1984. The GEM have attracted a new generation of molecular and cellular biologists eager to apply their skill sets to these surrogates of the human disease. Newcomers often enter the field without an appreciation of the origins of mouse mammary tumor biology and the basis for many of the prevailing concepts. Our purpose in writing this compendium is to extend an "olive branch" while simultaneously deepen the knowledge of the novice mouse mammary tumor biologist as they journey into a field rich in pathology and genetics spanning several centuries.

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Nonproliferating Cells of Tumors

Cited by 2 publications

References 0 publications

Embryonic Stem Cells Are Redirected to Non-Tumorigenic Epithelial Cell Fate by Interaction with the Mammary Microenvironment

Embryonic Stem Cells Are Redirected to Non-Tumorigenic Epithelial Cell Fate by Interaction with the Mammary Microenvironment

A Compendium of the Mouse Mammary Tumor Biologist: From the Initial Observations in the House Mouse to the Development of Genetically Engineered Mice

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