Nonmotile Cellulose Synthase Subunits Repeatedly Accumulate within Localized Regions at the Plasma Membrane in Arabidopsis Hypocotyl Cells following 2,6-Dichlorobenzonitrile Treatment

DeBolt, Seth; Gutierrez, Ryan; Ehrhardt, David; Somerville, Chris

doi:10.1104/pp.107.104703

Cited by 112 publications

(130 citation statements)

References 29 publications

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“…For instance, the inhibitor of microtubule polymerization, oryzalin, was useful in demonstrating that the CESA insertion and directional motility were independent of microtubules (Paredez et al, 2006;DeBolt et al, 2007b). As mentioned above, inhibiting actin polymerization using latrunculin B showed the requirement for actin in CESA trafficking (Wightman and Turner, 2008;Gutierrez et al, 2009).…”

mentioning

confidence: 99%

“…For instance, CGA, thaxtomin, and isoxaben cause clearance of CESA from the PM; therefore, either secretion of CESA is compromised or CESA is unable to assemble once at the PM (Paredez et al, 2006;Bischoff et al, 2009;Crowell et al, 2009). By contrast, DCB does not stop complexes from forming at the PM, but once there, CESA movement ceases and CESA hyperaccumulates (DeBolt et al, 2007b). Interestingly, DCB has been shown recently to bind to a microtubule-associated protein in hybrid aspen (Populus species), further supporting an action of the drug on the movement of CESA (Rajangam et al, 2008).…”

mentioning

confidence: 99%

“…Live cell imaging of CESA has led to a remarkable increase in our understanding of the enzyme's subcellular localization, regulation, trafficking, and guidance in growing cells during primary cell wall synthesis (Paredez et al, 2006;DeBolt et al, 2007aDeBolt et al, , 2007bDesprez et al, 2007;Persson et al, 2007;Crowell et al, 2009;Gutierrez et al, 2009) and in vascular tissue during secondary cell wall synthesis (Wightman and Turner, 2008). Labeling of the primary wall complex with YFP::CESA6 or GFP::CESA3 in Arabidopsis hypocotyl cells revealed discrete particles at the focal plane of the PM.…”

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confidence: 99%

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“…The disruption is, it can be assumed, the result of the inhibitory mechanism of the CBI. In the first group, isoxaben and numerous other compounds cause YELLOW FLUORES-CENT PROTEIN YFP):CESAs to be depleted from the PM and concomitantly accumulate in cytosolic vesicles (called small CESA compartments or microtubule-associated cellulose synthase compartments; Paredez et al, 2006;Crowell et al, 2009;Gutierrez et al, 2009) The second group, consisting only of dichlobenil (DCB), causes YFP: CESAs to become immobilized and hyperaccumulated at distinct foci in the PM (Herth, 1987;DeBolt et al, 2007b). The third group influences CSC-microtubule (MT)-associated functions resulting in errant movement and localization of YFP:CESAs (DeBolt et al, 2007a;Yoneda et al, 2007).…”

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Indaziflam Herbicidal Action: A Potent Cellulose Biosynthesis Inhibitor

Brabham¹,

Lei

et al. 2014

Plant Physiology

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Cellulose biosynthesis is a common feature of land plants. Therefore, cellulose biosynthesis inhibitors (CBIs) have a potentially broad-acting herbicidal mode of action and are also useful tools in decoding fundamental aspects of cellulose biosynthesis. Here, we characterize the herbicide indaziflam as a CBI and provide insight into its inhibitory mechanism. Indaziflam-treated seedlings exhibited the CBI-like symptomologies of radial swelling and ectopic lignification. Furthermore, indaziflam inhibited the production of cellulose within ,1 h of treatment and in a dose-dependent manner. Unlike the CBI isoxaben, indaziflam had strong CBI activity in both a monocotylonous plant (Poa annua) and a dicotyledonous plant (Arabidopsis [Arabidopsis thaliana]). Arabidopsis mutants resistant to known CBIs isoxaben or quinoxyphen were not cross resistant to indaziflam, suggesting a different molecular target for indaziflam. To explore this further, we monitored the distribution and mobility of fluorescently labeled CELLULOSE SYNTHASE A (CESA) proteins in living cells of Arabidopsis during indaziflam exposure. Indaziflam caused a reduction in the velocity of YELLOW FLUORESCENT PROTEIN:CESA6 particles at the plasma membrane focal plane compared with controls. Microtubule morphology and motility were not altered after indaziflam treatment. In the hypocotyl expansion zone, indaziflam caused an atypical increase in the density of plasma membrane-localized CESA particles. Interestingly, this was accompanied by a cellulose synthase interacting1-independent reduction in the normal coincidence rate between microtubules and CESA particles. As a CBI, for which there is little evidence of evolved weed resistance, indaziflam represents an important addition to the action mechanisms available for weed management.

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“…Due to these symptoms and to other physiological responses, it was stated that Integrative theory of the mode of action of quinclorac: literature review these responses would be associated to the inhibition of the cellular expansion (Koo et al, 1996). Similar effects are found in other herbicides that inhibit cell wall synthesis, such as oxaziclomefone (O'Looney & Fry, 2005) and diclobenil (Peng et al, 2001;DeBolt et al, 2007;Wightman & Turner, 2010).…”

Section: Effect On the Formation Of The Cellular Wallmentioning

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Integrative Theory of the Mode of Action of Quinclorac: Literature Review1

Fipke

Vidal

2016

Planta daninha

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-Quinclorac is a systemic herbicide absorbed by germinating seeds, roots and leaves of seedlings. It is a selective compound for crops such as rice, canola, barley, corn, sorghum, and pasture. Quinclorac can be used to control various monocots and dicotyledonous weed species. The biochemical function of this herbicide in the plant has intrigued scientists for nearly four decades. The objectives of this review are to present evidence of three hypotheses on the biochemical functioning of quinclorac and to propose an integrative mode of action. The first theory on the mode of action of quinclorac is supported by evidence of inhibition of incorporation of C 14 -glucose into cellulose and hemicellulose, thus, affecting the cell wall synthesis. The second hypothesis suggests that quinclorac acts as an auxin in broadleaved weed species. In grass species, however, this herbicide appears to stimulate the activity of the 1-aminocyclopropane-1-carboxylate synthase enzyme and, subsequently, to increase the ethylene production; also, it seems to increase the cyanide acid content to phytotoxic levels. A third hypothesis to explain the harmful effect in some plant species is the formation of reactive oxygen species (ROS). Apparently, these processes are not mutually exclusive; therefore, an integrative theory for the action of quinclorac is suggested. It is theorized that the aforementioned biochemical activities are interconnected and can be the phytotoxic backbone to explain the herbicidal effect depending on the plant species and the plant growth stage, among other factors.Keywords: cellulose biosynthesis-inhibitor, cyanide, auxinic herbicide, reactive oxygen species. Palavras-chave: inibidor da síntese de celulose, herbicida auxínico, cianeto, espécies reativas de oxigênio. RESUMO

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Nonmotile Cellulose Synthase Subunits Repeatedly Accumulate within Localized Regions at the Plasma Membrane in Arabidopsis Hypocotyl Cells following 2,6-Dichlorobenzonitrile Treatment

Cited by 112 publications

References 29 publications

Tools for Cellulose Analysis in Plant Cell Walls

Tools for Cellulose Analysis in Plant Cell Walls

Indaziflam Herbicidal Action: A Potent Cellulose Biosynthesis Inhibitor

Integrative Theory of the Mode of Action of Quinclorac: Literature Review1

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